We narrowed to 7,506 results for: PAC;

1. pAAV-syn-FLEX-synaptoPAC-minWPRE

   Plasmid

   #153100

   Purpose

   Cre-conditional expression of synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

   Depositor

   Dietmar Schmitz

   Article

   Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

   Inserts

   bPAC(WT)
   Synaptophysin (Syp Rat)
   

   Use

   AAV

   Tags

   His tag, mScarlet, and myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5 HA3-eDHFR-T2A-neo

   Plasmid

   #86396

   Purpose

   for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection

   Depositor

   David Bentley

   Article

   Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

   Insert

   HA3 eDHFR T2A neo
   

   Use

   CRISPR

   Tags

   HA3 eDHFR T2A neo

   Expression

   Mutation

   R12Y, G67S and Y100I mutations in E coli DHFR

   Promoter

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACEBac1-PARP1-Flag-His-7xKQ

   Plasmid

   #111573

   Purpose

   Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Tags

   C-terminal 6xHis and C-terminal Flag

   Expression

   Insect

   Mutation

   lysine to glutamine mutation at the following ami…

   Promoter

   polyhedrin promoter

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-mEGFP(A206K)-paCaMKII(T286A)

   Plasmid

   #165439

   Purpose

   Expresses photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII(T286A)
   

   Use

   Tags

   mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   T286A: Autophosphorylation deficient mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM-HE-Glyco-Venus-bPAC(S27A)

   Plasmid

   #165490

   Purpose

   Xenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   Glyco-Venus-bPAC(S27A)-Myc
   

   Use

   Cdna expression, xenopus oocyte

   Tags

   Myc and Venus

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBx1-VHH template1-3XMyc-Spacer

   Plasmid

   #176208

   Purpose

   PCR template for generating CeVICA VHH/nanobody library

   Depositor

   Aviv Regev

   Article

   Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

   Insert

   VHH template1-3XMyc-Spacer
   

   Use

   Tags

   Myc tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC012 LwCas13a locus with nontargeting spacer

   Plasmid

   #91904

   Purpose

   LwCas13a locus into pACYC184 with nontargeting spacer

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

   Insert

   LwCas13a with non-targeting spacer
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 16, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

   Plasmid

   #183605

   Purpose

   Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   EGFP-TM
   

   Use

   Tags

   HA, Human PDGFRB transmembrane domain, Mouse IgGK…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBx1-VHH template3-3XMyc-Spacer

   Plasmid

   #176210

   Purpose

   PCR template for generating CeVICA VHH/nanobody library

   Depositor

   Aviv Regev

   Article

   Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

   Insert

   VHH template3-3XMyc-Spacer
   

   Use

   Tags

   Myc tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBx1-VHH template2-3XMyc-Spacer

    Plasmid

    #176209

    Purpose

    PCR template for generating CeVICA VHH/nanobody library

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    VHH template2-3XMyc-Spacer
    

    Use

    Tags

    Myc tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPy-CAG-GFP-IRES-Pac

    Plasmid

    #48759

    Purpose

    CAG promoter driving GFP-IRES-Puromycin Acetyl Transferase

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Insert

    EGFP
    

    Use

    Ppy

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G

    Plasmid

    #146392

    Purpose

    Insect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

    Insert

    DmeIF4G-trunc (eIF4G1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    322-1666 truncated version of elF4G sequence with…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5.1B-lambdaN-HA-DmPAN3-W707AG711E-dsRNAres_Y

    Plasmid

    #148087

    Purpose

    Insect Expression of DmPAN3-W707AG711E-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN3-W707AG711E-dsRNAres (CG11486 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC010 LshCas13a locus with nontargeting spacer

    Plasmid

    #91901

    Purpose

    LshCas13a locus into pACYC184 with nontargeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LshCas13a locus with non-targeting spacer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAcGP67A human LRP6 E1E2 (20–629)

    Plasmid

    #132627

    Purpose

    expresses human LRP6 E1E2 (20–629) in Hi5 insect cell

    Depositor

    Wenqing Xu

    Article

    Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

    Insert

    LRP6 (20–629)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAcGP67A human LRP6 E1E4 (20–1244)

    Plasmid

    #132629

    Purpose

    expresses human LRP6 E1E4 (20–1244) in Hi5 insect cell

    Depositor

    Wenqing Xu

    Article

    Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

    Insert

    LRP6 (20–1244)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAC8_MF-P10-mCherry-pH-3C-TwinStrep (VE5621)

    Plasmid

    #139770

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter TwinStrep tag under the PH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    C-terminal TwinStrep tag
    

    Use

    Tags

    3C - TwinStrep tag

    Expression

    Mutation

    Promoter

    PH

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC8_MF-p10-10His-Cter (VE5742)

    Plasmid

    #139776

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    C-terminal 10His tag
    

    Use

    Tags

    10 His

    Expression

    Insect

    Mutation

    Promoter

    PH

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAC8_MF-p10-Flag-Cter(VE5738)

    Plasmid

    #161798

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the p10 promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    C-terminal Flag tag
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    p10

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAC8_MF-PH-HA-Nter-P10-mCherry (VE5740)

    Plasmid

    #161800

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a N-ter HA tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    N-terminal HA tag
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    Promoter

    PH

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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