We narrowed to 5,822 results for: pCas

1. pCA528 CHMP5 159-222

   Plasmid

   #108259

   Purpose

   Expresses C-terminal 159-222 residues of CHMP5 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-10

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP5 residues 148-219 (CHMP5 Human)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-U6-E690_guide-CBh-hSpCas9

   Plasmid

   #188545

   Purpose

   Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid E690

   Depositor

   Darrell Kotton

   Article

   Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   T2A-EGFP

   Promoter

   U6

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-U6-W308_guide-CBh-hSpCas9

   Plasmid

   #188546

   Purpose

   Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308

   Depositor

   Darrell Kotton

   Article

   Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   T2A-EGFP

   Promoter

   U6

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-U6-L101_guide-CBh-hSpCas9

   Plasmid

   #188547

   Purpose

   Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101

   Depositor

   Darrell Kotton

   Article

   Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   T2A-EGFP

   Promoter

   U6

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG SRGAP2A_EVH1(P339L F342C)

   Plasmid

   #222613

   Purpose

   Expresses a form of full length SRGAP2 with two point mutations (P339L and F342C) that abrogate EVH1 binding site for Homer1

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Point mutations P339L and F342C

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS puro 3XFLAG-hATXN7L3

   Plasmid

   #225725

   Purpose

   Transfection of ATXN7L3 with 3XFLAG tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   ATXN7L3 (ATXN7L3 Human)
   

   Tags

   3XFLAG

   Expression

   Mammalian

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS puro HA-hUSP27X S404N

   Plasmid

   #225724

   Purpose

   Transfection of USP27X (S404N) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X S404N (USP27X Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S404N

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS puro HA-hUSP27X Y381H

   Plasmid

   #225723

   Purpose

   Transfection of USP27X (Y381H) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X Y381H (USP27X Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y381H

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS puro HA-hUSP27X F313V

   Plasmid

   #225722

   Purpose

   Transfection of USP27X (F313V) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X F313V (USP27X Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   F313V

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS puro HA-hUSP27X K181E

    Plasmid

    #225721

    Purpose

    Transfection of USP27X (K181E) with HA tag in mammalian cells

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X K181E (USP27X Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K181E

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS puro HA-hUSP27X Y144C

    Plasmid

    #225720

    Purpose

    Transfection of USP27X (Y144C) with HA tag in mammalian cells

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X Y144C (USP27X Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y144C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS puro HA-hUSP27X G76S

    Plasmid

    #225719

    Purpose

    Transfection of USP27X (G76S) with HA tag in mammalian cells

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X G76S (USP27X Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G76S

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJS-BCD-Strep-PCaspase

    Plasmid

    #214034

    Purpose

    Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    PCaspase
    

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJS-BCD-Strep-dPCaspase

    Plasmid

    #214035

    Purpose

    Bacterial expression plasmid for strep-tagged catalytically dead PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    PCaspase
    

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    H79A, C146A

    Promoter

    T7 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-nEF-SpCas9D10A nickase

    Plasmid

    #216736

    Purpose

    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    EF-1-alpha core

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR8-PspCas13b_gRNA[ccdbCam]-2xPP7

    Plasmid

    #196846

    Purpose

    backbone for gRNA cloning of dpspCas13b tagged by 2xPP7

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCR8-PspCas13b_gRNA[ccdbCam]_1xMS2b

    Plasmid

    #196847

    Purpose

    backbone for gRNA cloning of dpspCas13b tagged by 1xMS2

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. gzk328-dpspCas13b_gRNA[ccdbCam]-10xPBSc_Loop

    Plasmid

    #196844

    Purpose

    backbone for gRNA cloning of dpspCas13b tagged by 10xPBSc_Loop

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. gzk326-dpspCas13b_gRNA[ccdbCam]-5xPBSc_Loop

    Plasmid

    #196845

    Purpose

    backbone for gRNA cloning of dpspCas13b tagged by 5xPBSc_Loop

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-AnkG ZU5in-3xHA

    Plasmid

    #213730

    Purpose

    Mammalian cell expression vector for overexpression of AnkG ZU5in isoform in motor neurons.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    AnkG ZU5in (Ank3 Mouse)
    

    Expression

    Mammalian

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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