We narrowed to 62,913 results for: cloning
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pcDNA3.2/v5-DEST hGlut2
Plasmid#18086DepositorInsertGlut2 (SLC2A2 Human)
ExpressionMammalianAvailable sinceFeb. 17, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-HA-USP43
Plasmid#22583DepositorPublicationInsertUSP43 (USP43 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationnot FL, missing first 108 aaAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP29
Plasmid#22599DepositorPublicationAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAd 2B Ngn3-I-nGFP
Plasmid#19410DepositorAvailable sinceNov. 10, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)
Plasmid#55217DepositorTypeEmpty backboneTagsSNAPf and TwinStrepIIExpressionInsectAvailable sinceJuly 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1187 VSVGmut
Plasmid#201913PurposeExpresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)DepositorInsertVSV-G (K47Q, R354A)
ExpressionMammalianMutationK47Q, R354APromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBITE-CD19-CD3
Plasmid#190677PurposeEncodes a modularized form of HD37-OKT3 bispecific antibodyDepositorInsertModularized-HD37-modularized-OKT3-P2A-NeonGreen
UseLentiviralTagsmNeonGreenExpressionMammalianPromoterEFSAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP
Plasmid#188778PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCmini-iCAP-AAV.MaCPNS1
Plasmid#185136Purposenon-standard AAV2 rep-AAV-MaCPNS1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-MaCPNS1 VP1 gene
UseAAVExpressionMammalianPromoterp41Available sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-CMV-Puro-AAVR-FLAG
Plasmid#166716PurposeVector expressing AAVR (KIAA0319L) with a C-terminal Flag tagDepositorAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSecUAG-Evol2
Plasmid#163148PurposeExpresses machinery necessary for selenocysteine incorporation at UAG codonsDepositorInsertsallo-tRNA UTu2D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
Cysteine desulfurase
ExpressionBacterialMutationCysteine 32 switched to Selenocysteine (UAG), Cys…PromoterEM7, araBAD, native As SelD promoter, and native …Available sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO.1 Puro shRNA Scramble
Plasmid#162011PurposeLentiviral negative control vector containing scramble shRNADepositorInsertscramble shRNA
UseLentiviralAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223 SARS-CoV-2 ORF8
Plasmid#141278PurposeGateway-compatible Entry vector, with insert of ORF8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertORF8 (ORF8 SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
R4pGWB601_UBQ10p-Turbo-YFP-NLS
Plasmid#127368PurposeBinary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plantsDepositorInsertTurboID (BirA mutant)
TagsGS linker, NLS, V5, and YFPExpressionPlantMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZS157 CRISPEY RT/Cas9
Plasmid#114454PurposeYeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expressionDepositorInsertSpCas9, Ec86-RT
UseCRISPRTagsSV40-NLSExpressionYeastMutationS. cerevisiae codon optimizedPromoterGAL1-GAL10Available sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKC-4.06
Plasmid#112084PurposeNMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)DepositorInsert3XFLAG firefly luciferase-beta-globin 39PTC (HBB Mouse, Human, Photinus pyralis)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiPGK Puro DEST ERKKTRClover
Plasmid#90227PurposeLentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsmCloverExpressionMammalianPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-AviTag-SnoopLigase
Plasmid#105626PurposePlasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylationDepositorInsertAviTag-SnoopLigase
ExpressionBacterialPromoterT7Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)
Plasmid#103849PurposedPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.DepositorInsertsUseCRISPRTagsGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…PromoterCMVAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by
