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GST-BHMT-I/G

Plasmid

#104442

Purpose

Autophagy cargo assay

Depositor

Carol Mercer

Article

Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

Insert

BHMT (BHMT Human)

Tags

GFP-myc GFP is from Aequorea victoria and GST

Expression

Mammalian

Mutation

N43A in GST (in a potential KFERQ-like sequence).…

Promoter

CMV

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX307 hDVL1

Plasmid

#102863

Purpose

Expresses human DVL1 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

human Dishevelled 1 (DVL1) (DVL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 2 to glycine. Likely due to poly…

Promoter

EF1A

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shSOD1

Plasmid

#102975

Purpose

Suppression of SOD1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shSOD1 (SOD1 Human)

Use

Lentiviral and RNAi

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA-32Asp8

Plasmid

#102617

Purpose

Expression of human RPA with aspartic acid substituted phosphorylation domain

Depositor

Marc Wold

Article

Binz et al J Biol Chem. 2003 Sep 12;278(37):35584-9

Inserts

RPA1 (RPA1 Human)
RPA3 (RPA3 )
RPA2 (RPA2 )

Expression

Bacterial

Mutation

with aspartic acid substituted phosphorylation do…

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET21 gamma-tubulin

Plasmid

#101825

Purpose

production of gamma-tubulin in bacteria

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

gamma-tubulin 1 (TUBG1 Human)

Tags

His

Expression

Bacterial

Mutation

Whole protein

Promoter

T7

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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TRAIP-SFB

Plasmid

#101769

Purpose

Mammalian expression of TRAIP fusion protein

Depositor

Michael Huen

Article

Feng et al Cell Discov. 2016 Jun 28;2:16016. doi: 1

Insert

TRAIP / RNF206 (TRAIP Human)

Tags

S peptide-flag-streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

siRNA-resistant allele

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pIRIGF-IKZF3

Plasmid

#69046

Purpose

expression of IKZF3 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF3 (IKZF3 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

IRES

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCLIPf-NK1R Control Plasmid

Plasmid

#101125

Purpose

Expression of CLIP-tagged Neurokinin-1 Receptor in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Neurokinin-1 Receptor, Truncated (TACR1 Human)

Tags

10xHis, CLIP-tag (CLIPf), and FLAG Epitope

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pLNCX2-NGN2-IRES-GFP-T2A-Sox11

Plasmid

#90213

Purpose

To convert human skin fibroblasts into induced cholinergic neurons (hiCN) in combination with FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Article

Liu et al Nat Commun. 2013;4:2183. doi: 10.1038/nc

Insert

NGN2-IRES-GFP-T2A-Sox11 (Sox11, NEUROG2 Human, Mouse)

Use

Retroviral

Promoter

CMV

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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PCBP1_pLX307

Plasmid

#98362

Purpose

Lentiviral expression of PCBP1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

PCBP1 (PCBP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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