We narrowed to 28,827 results for: PLE

1. pET28a_6HT-TBP6.7(WT)

   Plasmid

   #112720

   Purpose

   Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(WT)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_6HT-TBP6.7(P46A)

   Plasmid

   #112721

   Purpose

   This mutation (P46A) marks the first residue within the evolved region of U1A.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P46A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P46A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_6HT-TBP6.7(Q48T)

   Plasmid

   #112725

   Purpose

   This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48T)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48T

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a_6HT-TBP6.7(P51A)

   Plasmid

   #112728

   Purpose

   Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P51A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P51A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a_6HT-TBP6.7(Q54A)

   Plasmid

   #112730

   Purpose

   Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q54A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q54A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMS1957

   Plasmid

   #82568

   Purpose

   High copy number plasmid containing hs1218 MiniPromoter insert.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   hshs1218
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pEMS1953

   Plasmid

   #82567

   Purpose

   High copy number plasmid containing hs671 MiniPromoter insert.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   hs671
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pCIG1_UL136_25kDa_Myc

   Plasmid

   #74954

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)

   Expression

   Mammalian

   Mutation

   delta 1-77, M100A M128A

   Promoter

   CMV, Lentiviral LTR

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT

   Plasmid

   #61796

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutated

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Ywhab 3'UTR with miR-200c MRE-1 mutated
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   miR-200c MRE-1 mutated

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

10. psiCHECK-2-Xbp1-3'UTR-MRE-3 MUT

    Plasmid

    #61793

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Xbp1 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Xbp1 3'UTR with miR-200c MRE-3 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-3 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

11. psiCHECK-2-Smad5-3'UTR-MRE-1 MUT

    Plasmid

    #61791

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Smad5 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Smad5 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

12. psiCHECK-2-Map3k1-3'UTR-MRE-1 MUT

    Plasmid

    #61790

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Map3k1 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

13. psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT

    Plasmid

    #61789

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    both miR-200c MRE-2 and MRE-3 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

14. psiCHECK-2-Crtap-3'UTR-MRE-1 MUT

    Plasmid

    #61786

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Crtap with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Crtap 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.2-R5A-K6A C11orf83-V5

    Plasmid

    #65850

    Purpose

    Mammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5A-K6A

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    Mutated UQCC3 (UQCC3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R5A-K6A

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.2-R5D-K6D C11orf83-V5

    Plasmid

    #65851

    Purpose

    Mammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5D-K6D

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    Mutated UQCC3 (UQCC3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R5D-K6D

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. psiCHECK-2-Fhod1-3'UTR-MRE-2 MUT

    Plasmid

    #61787

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Fhod1 with MRE-2 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Fhod1 3'UTR with miR-200c MRE-2 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-2 mutated

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

18. psiCHECK-2-Tob1-3'UTR-MRE-1 MUT

    Plasmid

    #61788

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Tob1 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Tob1 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

19. psiCHECK-2-Zfp36-3'UTR-MRE-1 MUT

    Plasmid

    #61794

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Zfp36 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Zfp36 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

20. psiCHECK-2-Snail1-3'UTR-MRE-1 MUT

    Plasmid

    #61795

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Snail1 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Snail1 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

21. psiCHECK-2-Mapk12-3'UTR-MRE-1 MUT

    Plasmid

    #61797

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Mapk12 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Mapk12 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pGEM5Z(+)-EGFP

    Plasmid

    #65206

    Purpose

    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.

    Depositor

    LuZhe Sun

    Article

    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Restriction enzyme recognition sites added either…

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

    Plasmid

    #199371

    Purpose

    Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    14xHis-Avi

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T5-LacO

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. Plxna1-Fc-His

    Plasmid

    #72122

    Purpose

    Expresses the extracellular region of the PlexinA1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxna1 (Plxna1 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Plxna2-Fc-His

    Plasmid

    #72123

    Purpose

    Expresses the extracellular region of the PlexinA2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxna2 (Plxna2 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. STIM1-EGFP

    Plasmid

    #210370

    Purpose

    Expresses STIM1 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEMS1994

    Plasmid

    #49124

    Purpose

    AAV plasmid with S100B (Ple266) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple266-iCre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    S100B

    Available since

    May 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

    Plasmid

    #113865

    Purpose

    Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    CMV-eGFP
    TOP-iRFP
    PTCH1-mTurquoise2
    CBF-tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Flag-V0A

    Plasmid

    #210347

    Purpose

    Expresses ATP6V0A in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    V-ATPase V0A (ATP6V0A1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSPObooster

    Plasmid

    #216160

    Purpose

    Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains

    Depositor

    Grant Brown

    Article

    Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978

    Inserts

    MKT1(30G) (MKT1 Budding Yeast)
    RME1(ins-308A) (RME1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    MKT1 gene containing an aspartic acid to glycine …

    Promoter

    endogenous promoter

    Available since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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