We narrowed to 5,257 results for: phy

Showing: 1961 - 1980 of 5257 results

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pCS2 xRor2-mCherry

Plasmid

#67162

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

mCherry

Expression

Mammalian

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pQIq_MRGS_HIS8_(27G2)_FLAG

Plasmid

#199616

Purpose

Encodes DARPin-FLAG 27G2 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG 27G2

Tags

8xHis and FLAG

Expression

Bacterial

Promoter

T5

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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Keratin 18 R89C

Plasmid

#18059

Depositor

M Bishr Omary

Article

Ku et al J Cell Biol. 1994 Oct . 127(1):161-71.

Insert

Keratin 18 (KRT18 Human)

Expression

Mammalian

Mutation

R89C

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pMOS011E: Entry vector for: superecliptic pHluorin (mitochondrial)

Plasmid

#163067

Purpose

Entry vector for: superecliptic pHluorin (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Gateway Cloning

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFRETvr-2in1-CC

Plasmid

#105117

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mVenus/tagRFP

Expression

Plant

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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Mammalian_SaAID

Plasmid

#188653

Purpose

Expresses SaAID in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nSaCas9-AID-UGI

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

pCMV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pUG-248

Plasmid

#104709

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

PUB22 C13A (PUB22 Mustard Weed)

Use

Synthetic Biology

Mutation

C13A, BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-21

Plasmid

#104707

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

PUB22 WT (PUB22 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-UBC1

Plasmid

#104666

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC1 (UBC1 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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VV246: CoChR(C108S)-GCaMP6f-ER in fck

Plasmid

#58521

Purpose

Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

CoChR(C108S)-GCaMP6f-ER

Use

Lentiviral

Tags

GCaMP6f

Expression

Mammalian

Mutation

changed Cysteine 108 to Serine

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pSW549 YFP/mVenus

Plasmid

#115984

Purpose

entry vector for GreenGate cloning method, CDS

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

mVenus

Use

Unspecified

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCBC-DT1T2.2

Plasmid

#134752

Purpose

PCR template for sgRNA cloning

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

sgRNA-AtU6_29p

Use

CRISPR

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pGB411

Plasmid

#134760

Purpose

pCambia CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCBC-MT1T2.2

Plasmid

#134751

Purpose

PCR template for sgRNA cloning

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

sgRNA-TaU3p

Use

CRISPR

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Dendra2-Vinculin-23

Plasmid

#57748

Purpose

Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Vinculin (VCL Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pBAD-O-GECO1

Plasmid

#113938

Purpose

Bacterial expression of orange fluorescent Ca2+ sensor O-GECO1

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

O-GECO1

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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ATM N-terminal sgRNA

Plasmid

#207089

Purpose

pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ATCATTAAGTACTAGACTCA

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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MAC_C_STYK1

Plasmid

#187792

Purpose

MAC-tagged gene expression of human STYK1

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

STYK1 (STYK1 Human)

Expression

Mammalian

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLNCX Myr His p110 KD

Plasmid

#10837

Depositor

Thomas Roberts

Article

Auger et al Biochem Biophys Res Commun. 2000 Jun 16.

Insert

p110 KD (PIK3CA Bovine)

Use

Retroviral

Tags

6xHis and Myr

Expression

Mammalian

Mutation

D915S. Kinase Dead.

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
LZF80: Cre-on CAG-somCheRiff-CFP (DIO)

Plasmid

#104115

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

somCheRiff-CFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only