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pET51b(+)_MBP-nLucCP-65/66

Plasmid

#113920

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-65/66

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_MBP-nLucCP-65/68

Plasmid

#113921

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-65/68

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET51b(+)_MBP-nLucCP-67/68

Plasmid

#113922

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-67/68

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET51b(+)_S-Luc_CLIP

Plasmid

#113923

Purpose

Plasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tag

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc_CLIP

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET51b(+)_H-Luc

Plasmid

#113924

Purpose

Plasmid encoding the bacterial expression of the chimeric HaloTag7-NanoLucCP fusion dubbed H-Luc

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET51b(+)-LUCIDRS-MTX

Plasmid

#113925

Purpose

Plasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic biosensor for methotrexate

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

LUCID_RS-MTX

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-S-Luc_cyto

Plasmid

#113926

Purpose

Plasmid encoding the cytosolic localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-S-Luc_mito

Plasmid

#113927

Purpose

Plasmid encoding the mitochondrial localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc_mito

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-S-Luc_nucl

Plasmid

#113928

Purpose

Plasmid encoding the nuclear localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc_nucl

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-H-Luc_cyto

Plasmid

#113929

Purpose

Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-H-Luc_mito

Plasmid

#113930

Purpose

Plasmid encoding the mictochonfrial localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc_mito

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-H-Luc_nucl

Plasmid

#113931

Purpose

Plasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc_nucl

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
IFN-Beta_pGL3

Plasmid

#102597

Purpose

IFN-beta promoter driving luciferase. Use in IFN reporter assay.

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

Human IFN-Beta promoter (IFNB1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS_3E5E_luciferase

Plasmid

#103055

Purpose

Encodes Ebola virus minigenome with firefly luciferase reporter gene. Minigenome is under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

Ebolavirus minigenome

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-CMVtrans-FFLuc-polyA

Plasmid

#101156

Purpose

Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

Depositor

Marcel Bruchez

Article

Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

Insert

Firefly luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD2000

Plasmid

#101197

Purpose

Name: pBluescript-LANApi,K14. Bidirectional reporter driving LANApi side [green luciferase isoform from pCBG68] and K14 side [red luciferase isoform from pCBR] in pBluescript backbone.

Depositor

Dirk Dittmer

Article

Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

Insert

LANApi side [green luciferase isoform of pCBG68], K14 side [red luciferase isoform of pCBR]

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD2002

Plasmid

#101198

Purpose

Name: pCBG68-LANApi. LANApi driving green luciferase reporter for CBG68 in pCBG68 backbone.

Depositor

Dirk Dittmer

Article

Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

Insert

LANApi driving green luciferase reporter for CBG68 in pCBG68 backbone.

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD2005

Plasmid

#101219

Purpose

Name: pCBR-K14. K14 driving red luciferase reporter for CBR in pCBR backbone.

Depositor

Dirk Dittmer

Article

Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

Insert

K14 driving red luciferase reporter for CBR in pCBR backbone.

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD2015

Plasmid

#101365

Purpose

Name: pCBR-K14ΔTATA. K14 TATA box mutation in pCBR-K14 (pDD2005).

Depositor

Dirk Dittmer

Article

Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

Insert

K14 TATA box mutation in pCBR-K14 (pDD2005)

Use

Luciferase

Tags

Expression

Mutation

K14 TATA box mutation in pCBR-K14 (pDD2005)

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD2022

Plasmid

#101366

Purpose

Name: pCBG68-RBPjkΔB. RBPjk binding site B mutation in pCBG68-LANApi (pDD2002).

Depositor

Dirk Dittmer

Article

Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

Insert

RBPjk binding site B mutation in pCBG68-LANApi (pDD2002).

Use

Luciferase

Tags

Expression

Mutation

RBPjk binding site B mutation in pCBG68-LANApi (p…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge