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Addgene
Showing: 181 - 200 of 3546 results

  5. pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)

    Plasmid
    #115487
    Purpose
    CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    115487-plasmid-map-sequence-id-240326

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  8. pEG302 22aa SunTag NtDRMcd (no NLS) SUP

    Plasmid
    #115490
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    115490-plasmid-map-sequence-id-238520

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  9. pORFE0001

    Plasmid
    #112070
    Purpose
    AtCas9 module CDS1
    Depositor
    Christian Chevalier
    Insert
    AtCAS9
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized Cas9 S. pyogenes
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112070-plasmid-map-sequence-id-217624

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  10. pORFE0002

    Plasmid
    #112071
    Purpose
    AtCAS9 nickase D10A module CDS1
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 nickase D10A
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized nCas9 D10A S. pyogenes
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112071-plasmid-map-sequence-id-217629

    Enlarge

  11. pORFE0003

    Plasmid
    #112072
    Purpose
    AtDeadCAS9 D10A H840A module CDS1
    Depositor
    Christian Chevalier
    Insert
    AtDeadCAS9 D10A H840A
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112072-plasmid-map-sequence-id-217628

    Enlarge

  12. pORFE0005

    Plasmid
    #112073
    Purpose
    AtDeadCAS9 D10A H840A without stop codon module CDS1ns
    Depositor
    Christian Chevalier
    Insert
    AtDeadCAS9 D10A H840A without stop codon
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112073-plasmid-map-sequence-id-217642

    Enlarge

  13. pORFE0010

    Plasmid
    #112074
    Purpose
    3xmVenus module CT
    Depositor
    Christian Chevalier
    Insert
    3xmVenus
    Use
    Tags
    Expression
    Plant
    Mutation
    Ser to Thr at amino acid 63 and Phe to Leu at pos…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112074-plasmid-map-sequence-id-217639

    Enlarge

  14. pORFE0011

    Plasmid
    #112075
    Purpose
    pcUBI promoter module Pro+5U
    Depositor
    Christian Chevalier
    Insert
    pcUBI promoter
    Use
    Tags
    Expression
    Plant
    Mutation
    BsaI and BpiI restriction sites have been remove…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112075-plasmid-map-sequence-id-217625

    Enlarge

  15. pORFE0014

    Plasmid
    #112076
    Purpose
    AtCAS9 nickase D10A module CDS1ns
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 D10A without stop codon
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112076-plasmid-map-sequence-id-217621

    Enlarge

  16. pJPN0016

    Plasmid
    #112077
    Purpose
    tagRFP module CT
    Depositor
    Christian Chevalier
    Insert
    TagRFP
    Use
    Tags
    Expression
    Plant
    Mutation
    BsaI and BpiI restriction sites have been remove…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112077-plasmid-map-sequence-id-217643

    Enlarge

  17. pORFE1052

    Plasmid
    #112078
    Purpose
    Overexpression of the AtCAS9 nickase in plant under 2x35S promoter
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 D10A
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized nCas9 D10A S. pyogenes
    Promoter
    2x35S
    Availability
    Academic Institutions and Nonprofits only
    112078-plasmid-map-sequence-id-217613

    Enlarge

  18. pORFE1001

    Plasmid
    #112079
    Purpose
    Overexpression of the AtCAS9 in plant under 2x35S promoter
    Depositor
    Christian Chevalier
    Insert
    AtCAS9
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized Cas9 S. pyogenes
    Promoter
    2x35S
    Availability
    Academic Institutions and Nonprofits only
    112079-plasmid-map-sequence-id-217610

    Enlarge

  19. pJPN0003

    Plasmid
    #112080
    Purpose
    GFP module NT2
    Depositor
    Christian Chevalier
    Insert
    GFP
    Use
    Tags
    Expression
    Plant
    Mutation
    BsaI and BpiI restriction sites have been remove…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112080-plasmid-map-sequence-id-217645

    Enlarge

  20. pJPN0015

    Plasmid
    #112081
    Purpose
    tagRFP module NT2
    Depositor
    Christian Chevalier
    Insert
    TagRFP
    Use
    Tags
    Expression
    Plant
    Mutation
    BsaI and BpiI restriction sites have been remove…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112081-plasmid-map-sequence-id-217647

    Enlarge

Showing: 181 - 200 of 3546 results