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Showing: 1 - 20 of 1819 results

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pMS178

Plasmid

#215678

Purpose

Empty repair plasmid for ChrII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

5'HA + MCS + loxP + rps-0p::5'ΔHygR

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

5' ∆HYGR encodes aa1-226

Promoter

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pMS114

Plasmid

#215677

Purpose

Empty repair plasmid for ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

5'HA + MCS + loxN + rps-0p::5'ΔHygR

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

5' ∆HYGR encodes aa1-226

Promoter

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMS158

Plasmid

#215679

Purpose

Empty repair plasmid for ChrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

5'HA + MCS + lox2272 + rps-0p::5'ΔHygR

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

5' ∆HYGR encodes aa1-226

Promoter

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLT 653

Plasmid

#59996

Purpose

Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

Depositor

Lisa Timmons

Article

Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

Inserts

dhc-3 (dhc-3 Nematode)
klp-16 (klp-16 Nematode)

Use

Tags

Expression

Bacterial

Mutation

partial genomic

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ90 - Pmyo-2::mCherry::unc-54utr

Plasmid

#19327

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pmyo-2::mCherry

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pFS16 Pmyo-3 NPP-9 mChBioFLAG

Plasmid

#45099

Depositor

Steven Henikoff

Article

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

npp-9 (npp-9 Nematode)
unc-119 (unc-119 Nematode)

Use

Tags

BLRP, FLAG, and mCherry

Expression

Worm

Mutation

Promoter

myo-3 and unc-119

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pAS1-Prig-3::tagBFP

Plasmid

#165598

Purpose

rig-3 promoter derive BFP expression in AVA head neurons.

Depositor

Andrew Leifer

Article

Hallinen et al Elife. 2021 Jul 29;10:e66135. doi: 10.75

Insert

Prig-3, tagBFP

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-GFP-RGG

Plasmid

#124930

Purpose

Expression of RGG-GFP-RGG

Depositor

Matthew Good, Benjamin Schuster, Daniel Hammer

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ68 - Exp5605_Punc-122::GFP::unc-54utr

Plasmid

#19325

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Punc-122::GFP

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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BSP605

Plasmid

#122250

Purpose

expression vector for mCerulean under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mCerulean

Use

Tags

Expression

Worm

Mutation

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-gpa-2P::his-24::Cerulean

Plasmid

#124337

Purpose

Multicolor Worms for Neuronal ID

Depositor

Andrew Leifer

Insert

gpa-2, Cerulean (gpa-2 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-lim-4P::his-24::Cerulean

Plasmid

#124336

Purpose

Multicolor Worms for Neuronal ID

Depositor

Andrew Leifer

Insert

lim-4, Cerulean (lim-4 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMDJ39 - Peft-3 Cre (PATCs)

Plasmid

#191381

Purpose

High efficiency CRE recombinase expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Peft-3 Cre tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Peft-3

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pHW394 (15xUAS::GFP::let-858 3'UTR)

Plasmid

#85584

Purpose

cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

15xUAS-delta pes-10-GFP

Use

Tags

Expression

Worm

Mutation

GFP(S65C) with three introns for expression in C.…

Promoter

15xUAS-delta pes-10-GFP

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ104 - Pmyo-3::mCherry::unc-54

Plasmid

#19328

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pmyo-3::mCherry

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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pCFJ782 - HygroR

Plasmid

#190933

Purpose

Hygromycin resistance co-injection marker

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Hygromycin resistance gene

Use

Tags

Expression

Worm

Mutation

Promoter

rps-0

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pdestMB14_sur5p-dCas9-VP64

Plasmid

#177788

Purpose

Overexpression of dCas9-VP64 in C. elegans

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

dCas9-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS716

Plasmid

#188888

Purpose

Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

floxed Cbr-unc-119 marker and Phsp16.41::Cre

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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3xHA-TurboID_pAS31

Plasmid

#118220

Purpose

expresses 3xHA-tagged TurboID in C. elegans intestine

Depositor

Jessica Feldman

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Use

Tags

3x HA tag

Expression

Worm

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

ges-1p

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ350 - MCS(ttTi5605, II)

Plasmid

#34866

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi5605 targeting

Use

Worm targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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