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Plasmid#135992PurposeModular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterEF1a-shortAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only
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pOPTN-EGFP
Plasmid#27052DepositorInsertoptineurin (OPTN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
mApple-TOMM20-N-10
Plasmid#54955PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 568, Emission: 592DepositorInsertTOMM20 (TOMM20 Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_XS3B-miniXon_Luciferase
Plasmid#217302PurposePlasmid containing the SF3B3-XS3B/Firefly luciferase cassette, a modification of the SF3B3-miniXon system. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-XS3B by LDepositorInsertXS3B-miniXon
UseLuciferaseTagsExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRISPRoff-v2.1
Plasmid#167981PurposeExpresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoterDepositorInsertCRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
UseTags2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-shRNA-Control
Plasmid#98398PurposeLentivirus for expression of non-targeting control shRNA (Dox-inducible)DepositorInsertnon-targeting randomized sequence
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZW1-FCS-circRNA
Plasmid#73449PurposeExpression of circular RNADepositorInsertPOLR2A (POLR2A Human)
UseTagsnoneExpressionMammalianMutationnonePromoterCMV promoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-HsNRF2 (NFE2L2)
Plasmid#194303PurposeMammalian expression of human NRF2 (NFE2L2) fused to EGFP at the N-terminus. Parton lab clone KTHDepositorInsertNFE2L2 (NFE2L2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
TETv4
Plasmid#167983PurposeExpresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoterDepositorInsertTETv4 (TET1CD-XTEN80-dCas9)
UseCRISPR and Synthetic BiologyTags3xNLS, TET1 catalytic domain, and tagBFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Ubiquitin
Plasmid#18712PurposeMammalian expression of human ubiquitin with HA tagDepositorInsertubiquitin (UBB Human)
UseTagsHAExpressionMammalianMutationThe HA-Ubiquitin insert is from the UBB gene. The…PromoterAvailable sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-EGFP (BPK1098)
Plasmid#133962PurposeCMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcriptionDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-Tmem192-3xHA
Plasmid#102930PurposeExpress a lysosomal TagDepositorInsertTmem192 (TMEM192 Human)
UseLentiviralTagsHAExpressionMutationPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GenEPi
Plasmid#140236PurposeMammalian expression of Piezo1-based fluorescent force sensor GenEPiDepositorInsertGenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
UseTagsGCaMP 6s RS1 EF4ExpressionMammalianMutationPromotersimian CMV IE94Available sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-hTERT-IRES-hygro
Plasmid#85140PurposeLentiviral expression of hTERT, used to create immortalized cell linesDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-A3-HA-ubiquitin
Plasmid#220145PurposeExpresses A3-Ub for E3 substrate identification by E-STUBDepositorInsertUbiquitin C (UBC Human)
UseTagsA3-HAExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_FL_WT
Plasmid#98651PurposeExpresses MBP-tagged full length FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiMPH v2
Plasmid#89308Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.DepositorInsertMS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
UseLentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1aAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCCL-CellCycle
Plasmid#132429PurposeExpressing Fucci cell cycle indicator in mammalian cellsDepositorInserthCdt, hGeminin
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSI-Check2-hRluc-NFkB-firefly
Plasmid#106979PurposeLuciferase reporter with internal control for NFkB signal.DepositorInsertNFKB recognition sites (NFKB1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0079 RESCUE-S
Plasmid#130662PurposepCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16SDepositorInsertdRanCas13b-ADAR2dd
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only