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CapChR2_pmScarlet-N1

Plasmid

#188032

Purpose

Expresses CapChR2-mScarlet in mammalian cells

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR2-mScarlet

Use

Tags

mScarlet

Expression

Mammalian

Mutation

S43D, L112C, T139C and N238E mutations in CoChR

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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CapChR1_pmCherry-N1

Plasmid

#188031

Purpose

Expresses CapChR1-mCherry in mammalian cells

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR1-mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

S63D, L132C, T159C and N258E mutations in CrChR2

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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LMAPCW

Plasmid

#205655

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

mCMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAPcrRNA_Lmo

Plasmid

#126501

Purpose

L.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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sgAPC(A)

Plasmid

#85557

Purpose

Expresses sgRNA targeting human APC around codon I1417

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

APC, WNT signaling pathway regulator (APC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
sgAPC(B)

Plasmid

#85558

Purpose

Expresses sgRNA targeting human APC around codon I1417

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

APC, WNT signaling pathway regulator (APC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
Syn2ApcDNA4/TO

Plasmid

#14817

Depositor

Thomas Weimbs

Article

Low et al Dev Cell. 2003 May . 4(5):753-9.

Insert

Syntaxin2A (Stx2 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2007

Availability

Academic Institutions and Nonprofits only

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pAPcrRNA_Eco

Plasmid

#106276

Purpose

E.coli CRISPR Type I-E crRNA cloning vector for mammalian expression driven by U6 promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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KAPCA_HUMAN_D0

Plasmid

#79741

Purpose

This plasmid encodes the kinase domain of KAPCA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Nicholas Levinson, Markus Seeliger, John Chodera

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KAPCA

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
LAAPCW

Plasmid

#205691

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

AH

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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LHAPCW

Plasmid

#205682

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EH

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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LBAPCW

Plasmid

#205664

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CBH

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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MAPCW

Plasmid

#205700

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Retroviral

Tags

Expression

Mutation

Promoter

MSCV

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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LCAPCW

Plasmid

#205646

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hCMV

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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LEAPCW

Plasmid

#205673

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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APCSD 54

Plasmid

#66100

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for CSD 54

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
APCSD 53

Plasmid

#66099

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for CSD 53

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-Syn_CapChR2_eYFP

Plasmid

#188035

Purpose

Expresses CapChR2-eYFP in mammalian neurons

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR2-eYFP

Use

AAV

Tags

eYFP

Expression

Mutation

S43D, L112C, T139C and N238E mutations in CoChR

Promoter

hSyn

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBS Zpapc

Plasmid

#25774

Depositor

Edward De Robertis

Article

Yamamoto et al Development. 1998 Sep . 125(17):3389-97.

Insert

Papc (pcdh8 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only
PB-NES-ZapCV2

Plasmid

#222950

Purpose

Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

Depositor

Amy Palmer

Article

Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

Insert

NES-ZapCV2 (NES Synthetic)

Use

Tags

ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…

Expression

Mammalian

Mutation

ECFP gene is truncated 36 bp at 3' end. Venu…

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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