Showing: 1 - 20 of 27 results
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Plasmid#68338PurposeCampylobacter jejuni Cas9DepositorInsertCjCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
PX400 Campylobacter lari CF89-12 Cas9
Plasmid#68336PurposeCampylobacter lari CF89-12 Cas9DepositorInsertClCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCJR01
Plasmid#187888PurposeEscherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.DepositorInsertDNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
UseTagsExpressionBacterialMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
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pRGEN-CMV-CjCas9
Plasmid#89752PurposeExpression of CjCas9 in mammalian cellsDepositorInsertCas9 derived from Campylobacter jejuni
UseTagsSV40-HAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-CjCas9
Plasmid#89754PurposeExpression of CjCas9 with His tag in E.coliDepositorInsertCjCas9
UseTags6XHis, HA, and SV40 NLSExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pBlueKan+cysMPro
Plasmid#187879PurposeEscherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterCysMAvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-CjCAS9-pA
Plasmid#131543PurposeExpresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized CjCas9
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianMutationPromoterCAG, T3AvailabilityAcademic Institutions and Nonprofits only -
pCjeCas9
Plasmid#172215PurposeExpresses Campylobacter jejuni Cas9 (CjeCas9) in bacterial cellsDepositorInsertCas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
UseTagsHistagExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCjedCas9
Plasmid#172216PurposeExpresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cellsDepositorInsertcatalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
UseTagsHistagExpressionMutationchanged Asp 8 to Ala and His 559 to AlaPromoterAvailabilityAcademic Institutions and Nonprofits only -
T7-PseB-His6
Plasmid#89723PurposeExpresses PseB from C. jejuni with an N-terminal T7 tag and a C-terminal His6 tagDepositorInsertPseB
UseTagsHis6ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
GST-PglF130
Plasmid#89708PurposeExpresses truncated PglF from C. jejuni with N-terminal GST tagDepositorInsertpglF
UseTagsGSTExpressionBacterialMutationresidues 1-129 of putative transmembrane domain h…PromoterAvailabilityAcademic Institutions and Nonprofits only -
His8-TEV-PseC
Plasmid#89724PurposeExpresses PseC from C. jejuni with an N-terminal octaHis tag and TEV cleavage siteDepositorInsertPseC (pseC Campylobacter jejuni)
UseTagsHis8 and TEVExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
His8-TEV-PseH
Plasmid#89725PurposeExpresses PseH from C. jejuni with an N-terminal 2xHis Gln 5x His feature, followed by the TEV cleavage siteDepositorInsertPseH (pseH Campylobacter jejuni)
UseTags2xHis Gln 5xHis and TEVExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBEST-HipO
Plasmid#114599PurposeStrong constitutive expression of metabolic enzyme, HipO, for cell-free expressionDepositorInsertHipO
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterOR2-OR1-PrAvailabilityAcademic Institutions and Nonprofits only -
pU6-Cj-sgRNA
Plasmid#89753PurposeU6 promoter driven expression of Cj-SgRNA cloned with BsmbIDepositorInsertCj-SgRNA
UseSgrna expression under u6 promoterTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-CjCas9-eGFP-HIF1a
Plasmid#137929PurposeExpression of cjCas9 with Hif1a targeting sgRNADepositorInsertCjCas9
UseAAVTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
CMV-VPR-S-L1-dCjCas9 MiniCAFE
Plasmid#169910PurposeExpression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.DepositorInsertVPR-dCjCas9
UseCRISPRTagsExpressionMammalianMutationD8A in CjCas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pRGEN-CMV-VPR-L1-CjCas9 V-D
Plasmid#169913PurposeExpression of a VPR-dCjCas9 fusion protein to activate gene expression.DepositorInsertVPR-dCjCas9
UseCRISPRTagsExpressionMammalianMutationD8A, H559A in cjCas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Pdpy-30-miniCAFE
Plasmid#170115PurposeDpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.DepositorInsertTruncated VPR-dCjCas9 (MiniCAFE)
UseCRISPRTagsExpressionMutationD8A in CjCas9PromoterDpy-30AvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 27 results