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Showing: 81 - 100 of 250 results

  11. pMD19T-psba1-Ppsba2-dCas9-SpR

    Plasmid
    #73220
    Purpose
    Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
    Depositor
    Paul Hudson
    Article
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    dCas9 from S. pyogenes
    Use
    Tags
    c-myc
    Expression
    Bacterial
    Mutation
    Silent mutation at bp 1341 A->C to remove an E…
    Promoter
    PpsbA2
    Availability
    Academic Institutions and Nonprofits only
    73220-plasmid-map-sequence-id-134711

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  12. pMD19T-psba1-TetR-PL22-dCas9-SpR

    Plasmid
    #73223
    Purpose
    Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
    Depositor
    Paul Hudson
    Article
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    dCas9 from S. pyogenes
    Use
    Tags
    c-myc
    Expression
    Bacterial
    Mutation
    Silent mutation at bp 1341 A->C to remove an E…
    Promoter
    PL22
    Availability
    Academic Institutions and Nonprofits only
    73223-plasmid-map-sequence-id-135055

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  14. RNA polymerase beta prime subunit FLAG

    Plasmid
    #102337
    Purpose
    Targeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.
    Depositor
    Erin O'Shea
    Article
    Piechura et al Elife. 2017 Dec 14;6. pii: 32032. doi: 1
    Insert
    RNAP Beta Prime Subunit with C-terminal FLAG TAG
    Use
    Tags
    3x FLAG with 3X GSGS linker
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    102337-plasmid-map-sequence-id-197499

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Showing: 81 - 100 of 250 results