Showing: 41 - 60 of 97 results
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Plasmid#182685PurposeEncodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.DepositorInsertsUseTagsExpressionMammalianMutationPromoterCMV and NAAvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)
Plasmid#182287PurposeEncodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
UseTagsnuclear export signal (NES)ExpressionMammalianMutationY306A/Y384F (AF) double mutant of Methanosarcina …PromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)
Plasmid#182652PurposeEncodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
4xU6-tRNAM15
UseTagsnuclear export signal (NES)ExpressionMammalianMutationY306A/Y384F (AF) double mutant of Methanosarcina …PromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA
Plasmid#182653PurposeEncodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
mutant eukaryotic release factor 1
UseTagsHA tag and nuclear export signal (NES)ExpressionMammalianMutationE55D and Y306A/Y384F (AF) double mutant of Methan…PromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-sfCherry(1-10)
Plasmid#70222PurposeExpresses sfCherry(1-10) in mammalian cellsDepositorInsertsfCherry(1-10)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GFP(1-10)
Plasmid#70219PurposeExpresses GFP(1-10) in mammalian cellsDepositorHas ServiceCloning Grade DNAInsertGFP(1-10)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLNCX Myr His p110
Plasmid#10836DepositorInsertp110 (PIK3CA Bovine)
UseRetroviralTags6xHis and MyrExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLNCX Myr His p110 KD
Plasmid#10837DepositorInsertp110 KD (PIK3CA Bovine)
UseRetroviralTags6xHis and MyrExpressionMammalianMutationD915S. Kinase Dead.PromoterAvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-GFP1-10
Plasmid#80409PurposeExpresses GFP1-10 in mammalian cellsDepositorInsertGFP1-10
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pHRm-GFP(1-10)-VP64-NLS
Plasmid#70228PurposeExpresses GFP(1-10)-VP64-NLS in mammalian cellsDepositorInsertGFP(1-10)-VP64-NLS
UseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pYFP
Plasmid#175592PurposeTet inducible, KAN Resistant, mVenus gene on a low copy backbone. Used as a negative control to the pRelA_YFP plasmid.DepositorInsertmVenus
UseTagsExpressionBacterialMutationAn improved monomeric YFP. See doi: 10.1021/bi051…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCFP
Plasmid#175593PurposeLac inducible, AMP Resistant, cerulean gene on a low copy backbone. Used as a negative control to the pMesh1_CFP plasmid.DepositorInsertCFP (cerulean)
UseTagsExpressionMutationAn improved monomeric CFP. See doi: 10.1038/nbt945PromoterAvailabilityAcademic Institutions and Nonprofits only -
pENTR2B-dCas9-KRAB
Plasmid#115547PurposeEntry cassette for dCas9-KRABDepositorInsertdCas9-KRAB
UseTagsHAExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pACUH-GFP11x7-mCherry-α-tubulin
Plasmid#70218PurposeExpresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.DepositorInsertGFP11x7-mCherry-α-tubulin
UseTagsGFP11x7 and mCherryExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBEN276
Plasmid#69150PurposeIntegration of luciferase operon in Tn7 insertion site of Gram negative bacteriaDepositorInsertluxCDABE
UseLuciferaseTagsExpressionMutationPromoterfrrAvailabilityAcademic Institutions and Nonprofits only -
psPAX2-D64V-NC-COM
Plasmid#131226PurposeExpressing GAG precursor protein, in which COM is inserted after the second zinc finger domain of nucleocapsid protein (NC)DepositorInsertCOM
UseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
1056D
Plasmid#183132PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
1056E
Plasmid#183133PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[dsx]
UseCRISPRTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
1056F
Plasmid#183134PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[tra]
UseCRISPRTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMesh1*
Plasmid#175591PurposeLac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1
UseTagsFused with a flexible GS linker to ceruleanExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 41 - 60 of 97 results