Addgene

Showing: 21 - 40 of 109 results

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CaV2.1 (pCR3.1)

Plasmid

#26573

Depositor

Diane Lipscombe

Article

Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

Insert

Cacna1a (Cacna1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
CaV1.3e[8a,11,31b,Δ32,42a] mut

Plasmid

#26576

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Use

Tags

6XHis and V5

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Promoter

Availability

Academic Institutions and Nonprofits only
CaV2.1 (pcDNA6)

Plasmid

#26578

Depositor

Diane Lipscombe

Article

Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

Insert

Cacna1a (Cacna1a Mouse)

Use

Tags

His6 and V5

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
a1Ic-HE3-pcDNA3

Plasmid

#45810

Depositor

Edward Perez-Reyes

Article

Gomora et al Biophys J. 2002 Jul;83(1):229-41.

Insert

CACNA1I (CACNA1I Human)

Use

Making crna for oocyte injections

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP Cav2.2 HA pcDNA3

Plasmid

#206053

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP Cav2.2 HA pCAGGS

Plasmid

#206054

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Availability

Academic Institutions and Nonprofits only

Enlarge
EYFP Cav2.2 pRK5

Plasmid

#206099

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal EYFP tag and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
CaV1.3e[8a,11,31b,Δ32,Δ42a] mut

Plasmid

#26577

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Use

Tags

Expression

Mammalian

Mutation

Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
α1Ic-HE2-pcDNA3

Plasmid

#45808

Depositor

Edward Perez-Reyes

Article

Murbartian et al FEBS Lett. 2002 Sep 25;528(1-3):272-8.

Insert

α1Ic (Cacna1i Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
CaV1.3e[8a,11,31b,Δ32,42a]

Plasmid

#49333

Purpose

Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Use

Tags

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
CaV1.2/pcDNA3.1~ Human

Plasmid

#140576

Purpose

Expression in mammallian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

Insert

human CaV1.2 (CACNA1C Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pGW-Dronpa-Cav1.2

Plasmid

#182333

Purpose

L-type voltage-gated calcium channel α1C subunit tagged with Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

Cav1.2-Dronpa (CACNA1C Rabbit)

Use

Tags

Full-length rabbit Cav1.2.

Expression

Mammalian

Mutation

T1066Y, Q1070M

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Cav2.2 HA pcDNA3

Plasmid

#206051

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Cav2.2 HA pCAGGS

Plasmid

#206052

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Availability

Academic Institutions and Nonprofits only

Enlarge
Cav2.2 BBS pcDNA3

Plasmid

#206057

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Cav2.2 HA W391A pcDNA3

Plasmid

#206071

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Cav2.2 pcDNA3

Plasmid

#206072

Purpose

expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Cav2.1 HA pCAGGS

Plasmid

#206076

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1a (Cacna1a Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP Cav2.2 W391A pRK5

Plasmid

#206084

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Cav2.2 BBS exon37a pCAGGS

Plasmid

#206086

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and alternative exon 37a

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Availability

Academic Institutions and Nonprofits only

Enlarge