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Showing: 41 - 60 of 129 results

  5. pHD-dsRed-attP_dCrk_flankingHRs

    Plasmid
    #131141
    Purpose
    Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Inserts
    Crk Upstream Homology Region (Crk Fly)
    Crk Downstream Homology Region (Crk Fly)
    Use
    CRISPR and Cre/Lox; Homology directed repair temp…
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    131141-plasmid-map-sequence-id-258858

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  9. pSG5-Crkl mut SH2

    Plasmid
    #41703
    Depositor
    Nora Heisterkamp
    Insert
    Crkl (CRKL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R39K
    Promoter
    SV40
    Availability
    Academic Institutions and Nonprofits only

  10. pSG5-Crkl mut SH3

    Plasmid
    #41705
    Depositor
    Nora Heisterkamp
    Insert
    Crkl (CRKL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    W160K
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  12. pSG5-Crkl mut SH2/SH3

    Plasmid
    #41704
    Depositor
    Nora Heisterkamp
    Insert
    Crkl (CRKL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R39K and W160K
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  13. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

    Plasmid
    #131143
    Purpose
    Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    mNeonGreen::3XFLAG::Crk (Crk Fly)
    Use
    Cre/Lox; Attb recombination site
    Tags
    3XFLAG and mNeonGreen
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    131143-plasmid-map-sequence-id-258814

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  14. gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1)

    Plasmid
    #217340
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Availability
    Academic Institutions and Nonprofits only
    217340-plasmid-map-sequence-id-426478

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Showing: 41 - 60 of 129 results