Showing: 1 - 20 of 50 results
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Plasmid#216438PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter eno101
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailabilityAcademic Institutions and Nonprofits only -
pPTK003-2-pENO1
Plasmid#84962PurposeType 2, pENO1, Enolase 1 promoterDepositorInsertEnolase 1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationBsaI site removed (2350 g to c) and BsmBI site re…PromoterNonAvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_ENO1-Halo
Plasmid#175330PurposeProduction of HaloTag fusion proteinDepositorInsertENO1 (ENO1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-ENO1_sgRNA1
Plasmid#201592PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertENO1 (ENO1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-ENO1_sgRNA2
Plasmid#201593PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertENO1 (ENO1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pENO1-dTom-NATr
Plasmid#52208PurposeConstitutive expression of Candida codon-optimized dTomato with NAT selectable markerDepositorInsertdTomato
UseTagsExpressionBacterial and YeastMutationcodon optimized for Candida speciesPromoterENO1AvailabilityAcademic Institutions and Nonprofits only -
pENO1-BFP-NAT
Plasmid#50046PurposeExpression of BFP in Candida albicansDepositorInsertBFP
UseTagsExpressionYeastMutationPromoterpENO1AvailabilityAcademic Institutions and Nonprofits only -
pENO1-iRFP-NATr
Plasmid#129731PurposeIntegration plasmid to overexpress iRFP in Candida albicansDepositorInsertiRFP-Candida albicans codon-optimized
UseTagsExpressionYeastMutationPromoterENO1 from Candida albicansAvailabilityAcademic Institutions and Nonprofits only -
pTO135_pENO1-CaCas9-tCyc1
Plasmid#171103PurposeExpresses Cas9 in Candida aurisDepositorInsertCaCas9
UseTagsExpressionYeastMutationPromoterENO1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Ppre3-ITE-Teno1
Plasmid#127724PurposeYeast pathway position 1. ITE transcription unit with the PRE3 promoter and ENO1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPpre3AvailabilityAcademic Institutions and Nonprofits only -
p2.1 (pAGL2A)
Plasmid#27563DepositorInsertENO1 promoter (ENO1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSSa16
Plasmid#177372PurposeExpresses red-shifted luciferase under ENO1 promoter in Candida glabrataDepositorInsertCgLUCopt-mut2
UseTagsExpressionMutationPromoterENO1AvailabilityAcademic Institutions and Nonprofits only -
TFORF1125
Plasmid#143093PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertENO1 (ENO1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pTO149_RFP_CauNEO
Plasmid#177277PurposeEncodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.DepositorInsertRFP, CauNEO
UseTagsENO1-RFPExpressionBacterialMutationAll CUG Leucine codons were replaced with the syn…PromoterAgTEF2AvailabilityAcademic Institutions and Nonprofits only -
pV1081
Plasmid#111425PurposeSolo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1DepositorInsertCaCas9/sgADE-NT2
UseCRISPRTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pV1025
Plasmid#111424PurposeExpression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)DepositorInsertCaCas9
UseCRISPRTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKmK.P3
Plasmid#125036PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertENO1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pV1093
Plasmid#111428PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2
Plasmid#171104PurposeExpresses sgRNA (ENO1 gRNA) in Candida auris.DepositorInsertsgRNA
UseTagsExpressionYeastMutationPromoterADH1AvailabilityAcademic Institutions and Nonprofits only -
pV1200
Plasmid#111429PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 50 results