Addgene

We narrowed to 175 results for: leishmania

Showing: 1 - 20 of 175 results

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pSPneogRNAH

Plasmid

#63556

Purpose

Expresses gRNA in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

Insert

guide sequence

Use

Leishmania expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pSPneoHHgRNAH

Plasmid

#63557

Purpose

Expresses gRNA in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

Insert

guide sequence

Use

Leishmania expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pLdCN2

Plasmid

#125186

Purpose

Expresses Streptococcus pyogenes Cas9 (SpCas9) and two gRNAs in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al Methods Mol Biol. 2020;2116:199-224. doi

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Tags

Expression

Mutation

Promoter

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pLdSaCN

Plasmid

#123261

Purpose

Expresses Staphylococcus aureus Cas9 (SaCas9) and its gRNA in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Insert

gRNA and SaCas9

Use

CRISPR; Leishmania

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLdCN

Plasmid

#84290

Purpose

Express gRNA and Cas9 in Leishmania with Neomycin resistance

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Tags

Expression

Mutation

Promoter

L. donovani ribosome RNA promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLdCH

Plasmid

#84291

Purpose

Express gRNA and Cas9 in Leishmania with Hygromycin resistance

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Tags

Expression

Mutation

Promoter

L. donovani ribosome RNA promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL6000

Plasmid

#216797

Purpose

Platform for DiCre inducible expression of a gene of interest in Leishmania major - requires Leishmania strain expressing DiCre recombinase

Depositor

Jeremy Mottram

Article

Santos et al Mol Biochem Parasitol. 2017 Sep;216:45-4

Type

Empty backbone

Use

Cre/Lox; Leishmania dicre inducible expression

Tags

6xHA

Expression

Mutation

Promoter

rRNA promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLPhygCAS9

Plasmid

#63555

Purpose

Expresses CAS9 in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

Insert

Humanized Streptococcus pyogenes Cas9 from PX330 (Addgene)

Use

Leishmania expression

Tags

flag

Expression

Mutation

Promoter

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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D44_Ld_180090

Plasmid

#135858

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

alpha glucosidase II subunit, putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pSPneogRNA241510+MT

Plasmid

#84292

Purpose

Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

LdBPK_241510.1 targeting gRNA and LdMT targeting gRNA

Use

CRISPR; Leishmania donovani

Tags

Expression

Mutation

Promoter

L. donovani ribosome RNA promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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D92_Ld_360170

Plasmid

#135905

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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D38_Ld_341960

Plasmid

#135852

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

thioredoxin, putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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D78_Ld_362280

Plasmid

#135891

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

transmembrane emp24 domain-containing protein

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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D86_Ld_260650

Plasmid

#135899

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

Protein of unknown function (DUF2012), putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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D32_Ld_354780

Plasmid

#135848

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

Hsp70 protein, putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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D63_Ld_100680

Plasmid

#135876

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

flagellar glycoprotein-like protein

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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D72_Ld_231020

Plasmid

#135885

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, unknown function

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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D76_Ld_303190

Plasmid

#135889

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

Translocon-associated protein beta (TRAPB), putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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D62_Ld_362720

Plasmid

#135875

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

membrane-bound acid phosphatase 2, putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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D57_Ld_190640

Plasmid

#135870

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

extracellular receptor, putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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