Showing: 81 - 100 of 137 results
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Plasmid#159945Purposeoptical regulation of nuclear exportDepositorInsertNLS-mTFP1.0-optoNES
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1AvailabilityAcademic Institutions and Nonprofits only -
psiCheck2-SV40p-3xFLAG-CMVp-mito-mTFP1
Plasmid#182376PurposeDual expression construct encoding 3xFLAG and mito-mTFP1 from separate promotersDepositorInserts3xFlag
mito-mTFP1
UseTagsExpressionMammalianMutationPromoterCMV promoter and SV40 promoterAvailabilityAcademic Institutions and Nonprofits only -
aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)
Plasmid#101297PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-906 of alpha-CateninPromoterAvailabilityAcademic Institutions and Nonprofits only -
aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)
Plasmid#101298PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-698 of alpha-CateninPromoterAvailabilityAcademic Institutions and Nonprofits only -
aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)
Plasmid#101299PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-698 and 698-906 of alpha-C…PromoterAvailabilityAcademic Institutions and Nonprofits only -
psiCheck2-SV40p-mtIF3(SR)-3xFLAG-CMVp-mito-mTFP1
Plasmid#182380PurposeDual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promotersDepositorInsertsUseTags3xFlagExpressionMammalianMutationChanged CDS sequence from 'ccacgttcaagtcacga…PromoterCMV promoter and SV40 promoterAvailabilityAcademic Institutions and Nonprofits only -
CN2700: rAAV-eHGT_452h-minBglobin-mTFP1-WPRE3-BGHpA
Plasmid#191708PurposeAI plasmid ALIAS: AiP12700. Expression of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)InsertmTFP1
UseAAVTagsExpressionMutationnonePromoterAvailabilityAcademic Institutions and Nonprofits only -
CN2965: rAAV-eHGT_444h-minBglobin-mTFP1-WPRE3-BGHpA
Plasmid#191729PurposeAI plasmid ALIAS: AiP12965. Expression of mTFP1 (monomeric teal fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs), enrichment in dorsal striatum over ventral striatumInsertmTFP1
UseAAVTagsExpressionMutationnonePromoterAvailabilityAcademic Institutions and Nonprofits only -
CN3754: rAAV-3xcore2-eHGT_779m-minBglobin-mTFP1-WPRE3-BGHpA
Plasmid#191733PurposeAI plasmid ALIAS: AiP13754. Expression of mTFP1 (monomeric teal fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)InsertmTFP1
UseAAVTagsExpressionMutationnonePromoterAvailabilityAcademic Institutions and Nonprofits only -
psiCheck2-SV40p-5'UTR-mtIF3(SR)-3xFLAG-3'UTR-CMVp-mito-mTFP1
Plasmid#182378PurposeDual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promotersDepositorInsertsUseTags3xFlagExpressionMammalianMutationChanged CDS sequence from 'ccacgttcaagtcacga…PromoterCMV promoter and SV40 promoterAvailabilityAcademic Institutions and Nonprofits only -
FRP265
Plasmid#159301PurposeYeast tagging vector to create gene fusion with yeast codon optimized mTFP1 under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-04
Plasmid#119983PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mTFP1DepositorInsertmTFP1
UsePcr templateTagsmTFP1ExpressionMutationPromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P04
Plasmid#120015PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mTFP1DepositorInsertmTFP1
UsePcr templateTagsmTFP1ExpressionMutationPromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z04
Plasmid#120047PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mTFP1DepositorInsertmTFP1
UsePcr templateTagsmTFP1ExpressionMutationPromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
pFUGW-FIRE-pHLy
Plasmid#170774PurposeRatiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterhUbCAvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FIRE-pHLy
Plasmid#170775PurposeRatiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I tension sensor (F40-based)
Plasmid#119186PurposeThe F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.DepositorInserthuman Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I no force control (F40-based)
Plasmid#119187PurposeThe no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between mTFP1 and mEYFP that are tension-independent.DepositorInserthuman Desmoplakinhuman Desmoplakin I (1-1945)-[mTFP1-F40-mEYFP] (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationtruncation after aa1945PromoterTCEAvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I donor only control (F40-based tension sensor)
Plasmid#119188PurposeThe donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.DepositorInserthuman Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I acceptor only control (F40-based tension sensor)
Plasmid#119189PurposeThe acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.DepositorInserthuman Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailabilityAcademic Institutions and Nonprofits only
Showing: 81 - 100 of 137 results