Showing: 1 - 20 of 33 results
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MLH1
Plasmid#28120PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMLH1:JMC01L-F09:C205690 (MLH1 Human)
UseTagsHis tag with TEV siteExpressionBacterialMutationcontains amino acid residues 1-347.PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCEP9 MLH1
Plasmid#16458DepositorInsertMLH1 (MLH1 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
AcGFP-MLH1
Plasmid#215120PurposeExpresses AcGFP-MLH1 in mammalian cells from a retroviral vector.DepositorInsertMLH1
UseRetroviralTagsAcGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMLH1
Plasmid#129426PurposeExpression human MLH1 protein using baculovirusDepositorInserthuman MLH1 (MLH1 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrin promoterAvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD051-sgMLH1-1
Plasmid#125776Purposeconstitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9DepositorInsertsgMLH1-1 (MLH1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD051-sgMLH1-2
Plasmid#125777Purposeconstitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9DepositorInsertsgMLH1-2 (MLH1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
373_MLH1 in pmiRGlo
Plasmid#78133Purposeluciferase reporter for miRNA activityDepositorInsertMLH1 (MLH1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation3'UTR containing miRNA binding sitesPromoterPGKAvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1dn
Plasmid#174824PurposeMammalian expression of human MLH1dn (codon optimized)DepositorInserthuman MLH1dn (codon optimized)
UseTagsExpressionMammalianMutationdeletion of residues 754-756PromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pEF1a-mMLH1dn
Plasmid#174825PurposeMammalian expression of mouse MLH1dn (codon optimized)DepositorInsertmouse MLH1dn (codon optimized)
UseTagsExpressionMammalianMutationdeletion of residues 758-760PromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1dn (og codon)
Plasmid#174823PurposeMammalian expression of human MLH1dn (original codon usage)DepositorInserthuman MLH1dn (original codon usage) (MLH1 Human)
UseTagsExpressionMammalianMutationdeletion of residues 754-756PromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1NTD-NLS
Plasmid#174826PurposeMammalian expression of human MLH1NTD-NLS (codon optimized)DepositorInserthuman MLH1_NTD-NLS (codon optimized)
UseTagsSV40 bpNLSExpressionMammalianMutationtruncation of residues 1-335PromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pLenti_Ef1a:PE2-P2A-hMLH1dn
Plasmid#184444PurposeSingle EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and Ef1AAvailabilityAcademic Institutions and Nonprofits only -
pT7-hMLH1dn for IVT
Plasmid#178114PurposeTemplate for in vitro transcription of human MLH1dnDepositorInserthuman MLH1dn (codon optimized) (MLH1 Human)
UseTemplate for in vitro transcriptionTagsExpressionMutationDetailed in manuscriptPromoterT7 (inactivated)AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-hMLH1dn
Plasmid#174827PurposeMammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)DepositorInsertPE2-P2A-hMLH1dn
UseTagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscript; deletion of residues 754-…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-hMLH1dn
Plasmid#174828PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)DepositorInsertPEmax-P2A-hMLH1dn
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDetailed in manuscript; deletion of residues 754-…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-hMLH1dn-eGFP
Plasmid#191104PurposeLentiviral expression plasmid of hMLH1dn with P2A-eGFP reporterDepositorInserthMLH1dn
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-hMLH1dn-Hygro
Plasmid#195512PurposeLentiviral expression plasmid of hMLH1dn with P2A-HygroR markerDepositorInserthMLH1dn-P2A-HygroR (MLH1 Human)
UseLentiviralTagsP2AExpressionMammalianMutationHuman MLH1 del754-756PromoterEF-1aAvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLV-gag-hMLH1dn
Plasmid#195513PurposeMMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.DepositorInserthMLH1dn (MLH1 Human)
UseVirus like particleTagsFLAG, NES, and Protease cleavate siteExpressionMammalianMutationHuman MLH1 del754-756PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-MLH1dn-IRES-puroR
Plasmid#207354PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).DepositorInsertMutL homolog 1 (MLH1 Human)
UseLentiviralTagsExpressionMutationΔ754-756 dominant negative mutantPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 33 results