Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 61 - 79 of 79 results

  2. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid
    #133794
    Purpose
    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018
    Depositor
    Benjamin Kleinstiver, Joseph Bondy-Denomy
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    NmeCas9 and Nme2Cas9 sgRNA entry vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Amrani et al. Genome Biol…
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    133794-plasmid-map-sequence-id-268076

    Enlarge

  4. AA296

    Plasmid
    #216028
    Purpose
    Fragmid fragment: (Cas protein) NNNNCC PAM
    Depositor
    John Doench
    Insert
    Nme2Cas9_v1.1 [Nme2]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216028-plasmid-map-sequence-id-431533

    Enlarge

  5. AA303

    Plasmid
    #216071
    Purpose
    Fragmid fragment: (guide cassette) guide expression
    Depositor
    John Doench
    Insert
    U6_v1; BsmBI_v0; BsmBI_v6; trRNA_v1 [Nme2]; terminator_v1
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216071-plasmid-map-sequence-id-431513

    Enlarge

  6. TFORF1215

    Plasmid
    #141803
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NME2 (NME2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    141803-plasmid-map-sequence-id-415121

    Enlarge

  7. TFORF3528

    Plasmid
    #145004
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NME2 (NME2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    145004-plasmid-map-sequence-id-287126

    Enlarge

  18. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

    Plasmid
    #199261
    Purpose
    Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    U1a promoter
    Availability
    Academic Institutions and Nonprofits only
    199261-plasmid-map-sequence-id-389819

    Enlarge

Showing: 61 - 79 of 79 results