Showing: 21 - 40 of 49 results
-
Plasmid#118196PurposeCRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 OPTN TSS-guide2
Plasmid#118197PurposeCRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 OPTN TSS-guide3
Plasmid#118198PurposeCRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 OPTN TSS-guide4
Plasmid#118199PurposeCRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6AvailabilityAcademic Institutions and Nonprofits only -
pBMN mEGFP-OPTN(F178A)
Plasmid#119681PurposeFor mammalian expression of LIR mutant OPTNDepositorInsertOPTN (OPTN Human)
UseRetroviralTagsmEGFPExpressionMammalianMutationF178APromoterAvailabilityAcademic Institutions and Nonprofits only -
pBMN mCherry-OPTN(D474N)
Plasmid#119683PurposeFor mammalian expression of UBD mutant OPTNDepositorInsertOPTN (OPTN Human)
UseRetroviralTagsmCherryExpressionMammalianMutationD474NPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBMN iRFP670-OPTN(D474N)
Plasmid#119693PurposeFor mammalian expression of iRFP-tagged UBD mutant OPTNDepositorInsertOPTN (OPTN Human)
UseRetroviralTagsiRFP670ExpressionMammalianMutationD474NPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-OPTN
Plasmid#175760PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-OPTNDepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-OPTN D474N
Plasmid#175762PurposeLentiviral vector expressing N-terminally tagged eGFP-OPTN D474NDepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationD474NPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-OPTN S513A
Plasmid#175764PurposeLentiviral vector expressing N-terminally tagged eGFP-OPTN S513ADepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationS513APromoterAvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-OPTN E50K
Plasmid#175765PurposeLentiviral vector expressing N-terminally tagged eGFP-OPTN E50KDepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationE50KPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGST2-GST-TEV-OPTN
Plasmid#198428PurposeUsed for the expression and purification of OPTN (WT) (SMC Internal No. 1587)DepositorInsertoptineurin (OPTN Human)
UseTagsGST and TEVExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-Duet_OPTN-thrombin-GST
Plasmid#216843PurposeFor expression in E. coli of OPTN (Internal ref. SMC1789)DepositorInsertOptineurin (OPTN Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pBMN mCherry-OPTN(F178A/D474N)
Plasmid#119684PurposeFor mammalian expression of LIR/UBD double mutant OPTNDepositorInsertOPTN (OPTN Human)
UseRetroviralTagsmCherryExpressionMammalianMutationF178A/D474NPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBMN iRFP670-OPTN(F178A/D474N)
Plasmid#119694PurposeFor mammalian expression of iRFP-tagged LIR/UBD double mutant OPTNDepositorInsertOPTN (OPTN Human)
UseRetroviralTagsiRFP670ExpressionMammalianMutationF178A/D474NPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-OPTN S473A 513A
Plasmid#175763PurposeLentiviral vector expressing N-terminally tagged eGFP-OPTN S473A 513ADepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationS473A S513APromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-6xHis-TEV-OPTN
Plasmid#190192PurposePlasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397DepositorInsertoptineurin (OPTN Human)
UseTags6xHis and TEVExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-OPTN (2-119)
Plasmid#208867PurposeStably express GFP-tagged OPTN (2-119aa) for CID induced mitophagyDepositorInsertOPTN (OPTN Human)
UseTagsFKBP-GFPExpressionMammalianMutationaa 2-119 onlyPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGST2-GST-TEV-OPTN S177D S473D
Plasmid#187827PurposePlasmid for the expression and purification of GST-TEV-OPTN S177D S473D. Internal Ref: SMC1588DepositorInsertOPTN (OPTN Human)
UseTagsGST-TEVExpressionBacterialMutationS177D S473DPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-His-TEV-mCherry-OPTN WT
Plasmid#190191PurposePlasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797DepositorInsertoptineurin (OPTN Human)
UseTags6xHis, TEV, and mCherryExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 40 of 49 results