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Showing: 61 - 80 of 121 results

  4. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Use
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Availability
    Academic Institutions and Nonprofits only
    182287-plasmid-map-sequence-id-363047

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  5. pcDNA3.1-CMV-CFP;UBC-Cre25nt

    Plasmid
    #65727
    Purpose
    Expresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.
    Depositor
    Jacco van Rheenen
    Article
    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1
    Inserts
    CMV promoter driving CFP expression
    UBC promoter driving Cre-25nt expression
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and UBC
    Availability
    Academic Institutions and Nonprofits only
    65727-plasmid-map-sequence-id-243566

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  6. pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

    Plasmid
    #182652
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    4xU6-tRNAM15
    Use
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Availability
    Academic Institutions and Nonprofits only
    182652-plasmid-map-sequence-id-362920

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  7. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Use
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

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  8. pMJA251

    Plasmid
    #128540
    Purpose
    Acceptor vector for gamma and Delta TCR
    Depositor
    Marcos Alcocer
    Insert
    truncated human TCR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional CMV
    Availability
    Academic Institutions and Nonprofits only
    128540-plasmid-map-sequence-id-251681

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  9. pMJA252

    Plasmid
    #128541
    Purpose
    Acceptor vector for alpha and beta TCR
    Depositor
    Marcos Alcocer
    Insert
    truncated human TCR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional CMV
    Availability
    Academic Institutions and Nonprofits only
    128541-plasmid-map-sequence-id-251633

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  10. Cavb3

    Plasmid
    #26574
    Depositor
    Diane Lipscombe
    Insert
    calcium channel beta subunit-III (Cacnb3 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26574-plasmid-map-sequence-id-148124

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  11. pMJA290

    Plasmid
    #128542
    Purpose
    Expresses nut lipid binding TCR
    Depositor
    Marcos Alcocer
    Insert
    Lipid binding Human TCR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional CMV
    Availability
    Academic Institutions and Nonprofits only
    128542-plasmid-map-sequence-id-251560

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  12. pMJA295

    Plasmid
    #128543
    Purpose
    Expresses nut lipid binding TCR
    Depositor
    Marcos Alcocer
    Insert
    Lipid binding Human TCR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional CMV
    Availability
    Academic Institutions and Nonprofits only
    128543-plasmid-map-sequence-id-251874

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  13. pMJA297

    Plasmid
    #128544
    Purpose
    Expresses nut lipid binding TCR
    Depositor
    Marcos Alcocer
    Insert
    Lipid binding Human TCR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional CMV
    Availability
    Academic Institutions and Nonprofits only
    128544-plasmid-map-sequence-id-251616

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Showing: 61 - 80 of 121 results