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We narrowed to 306 results for: SPI;

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Showing: 1 - 30 of 306 results

  2. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ...SARS-CoV-2 spike variants. “We created a series of spike expressing plasmids which encode spikes found on... Figure 1: Neutralization levels against spike pseudovirus decreases depending on the variant. ...Garcia-Beltran et al., 2021 with permission.   The spike variant pseudovirus neutralization assay Balazs’..., 2021 with permission.   Mutations in the spike protein are one of the key drivers in determining...

  3. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...Huber S, Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA (2018... identified nanobodies that bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the ...enter host cells. To do this, they injected the spike protein from two different coronaviruses into a ...isolated special camelid antibodies that bind to the spike proteins in cell culture, and inhibit these viruses...generate sybodies, synthetic nanobodies against the spike protein (Walter et al., 2020). No llamas required...Receptor Binding Domain found on the membrane-bound spike protein, in a remarkable 12 working days. While ...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...PRKCG MTH Spinocerebellar ataxia 30 Frederic Mushinski 8418 pLTR PKC gamma PRKCG Spinocerebellar ataxia ...GFP CMV Spinocerebellar ataxia 27 Tobias Meyer 21204 GFP-N2-PKCgamma PRKCG GFP CMV Spinocerebellar ataxia...GFP CMV Spinocerebellar ataxia 25 Tobias Meyer 21215 GFP-C2gamma PRKCG GFP CMV Spinocerebellar ataxia ...CMV Spinocerebellar ataxia 23 Bernard Weinstein 21237 PKC gamma dNPS PRKCG HA CMV Spinocerebellar ataxia... CMV Spinocerebellar ataxia 21 Bernard Weinstein 21239 PKC gamma DN PRKCG HA CMV Spinocerebellar ataxia...CMV Spinocerebellar ataxia 15 Ryohei Yasuda 112270 CMV-PKCg-mEGFP-N1 PRKCG GFP CMV Spinocerebellar ataxia...GFP CMV Spinocerebellar Ataxia Richard Youle 176464 pVPS13D-I-EGFP VPS13D GFP CMV Spinocerebellar Ataxia...

  5. COVID-19 Resources

    Type
    Collection
    ...all spike expression plasmids in our collection that are suitable for generating SARS-CoV-2 spike ...SARS-CoV-2 Spike (S) Ectodomain and RBD Libraries Screening Timothy Whitehead Libraries of Spike (S) Ectodomain...Bloom Library of Spike protein RBD mutations for comprehensive mapping. SARS-CoV-2 Spike Receptor Binding...entry driven by the severe acute respiratory syndrome coronavirus spike protein . Journal of virology, ...SARS-CoV and MERS CoV, depends on binding of the viral spike (S) protein to cellular receptors. The cellular ...expressing genes encoding SARS-CoV-2 proteins, (spike protein, S; small envelope protein, E; nucleocapsid... E. coli , yeast, and mammalian expression, and spike protein constructs for making...

  6. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Iterative Synthetically Phosphorylated Isomers (iSPI) The iSPI resource described in Galloway et al. (2022)...Pooled Library 188536 iSPI_pSer_Full_library Pooled Library 188535 iSPI_pSer_Subpool#10 Pooled Library 188534... 188534 iSPI_pSer_Subpool#9 Pooled Library 188533 iSPI_pSer_Subpool#8 Pooled Library 188532 iSPI_pSer_...Pooled Library 188531 iSPI_pSer_Subpool#6 Pooled Library 188530 iSPI_pSer_Subpool#5 Pooled Library 188529...188529 iSPI_pSer_Subpool#4 Pooled Library 188528 iSPI_pSer_Subpool#3 Pooled Library 188527 iSPI_pSer_Subpool...using iSPI, rEcoli XPS, and pSerOTS-C1* (V70) (ca. 2022) from the Rinehart lab: Methods for iSPI, rEcoli...phosphoprotein expression in rEcoli XpS in the Methods for iSPI, rEcoli XPS, pSerOTS-C1* (V70) (DOCX, 551 KB) . ...

  7. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Plasmids Expressing Spike and Spike Variants Full-length wildtype SARS-CoV-2 spike protein is inefficient...Browse Addgene's COVID-19 collection of SARS-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids...plasmids for viral pseudotyping with SARS-CoV-2 spike protein. Collections...Pseudotyping SARS-CoV-2 Pseudotyped Virus SARS-CoV-2 Spike Plasmids Accessory Plasmids Other Resources Mechanisms...replication-defective virus particles with the SARS-CoV-2 spike (S) glycoprotein on the envelope surface. Pseudotyped...particles have been pseudotyped with the SARS-CoV-2 Spike protein and you will find examples of each in Addgene's.... Use the search bar to find a particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant...

  8. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...-BGHpA AiP13044 AiE0779m_3xC2 SYFP2 DRD1 Medium spiny neurons (D1-MSN) Striatum 199776 pAAV-AiE0779m_3xC2...R297T)-BGHpA AiP13779 AiE0779m_3xC2 Cre DRD1 Medium spiny neurons (D1-MSN) Striatum 214855 pAAV-AiE0779m_3xC2...minBG-FlpO-WPRE3-BGHpA AiP14201 AiE0779m_3xC2 FlpO DRD1 Medium spiny neurons (D1-MSN) Striatum 224206 pAAV-AiE0780m-minCMV-SYFP2...WPRE3-BGHpA AiP15827 AiE0780m SYFP2 DRD1 Medium spiny neurons (D1-MSN) Striatum 224200 pAAV-AiE0780m-minBG-iCre...(R297T)-BGHpA AiP15578 AiE0780m Cre DRD1 Medium spiny neurons (D1-MSN) Striatum 191707 pAAV-AiE0452h-minBG-SYFP2...WPRE3-BGHpA AiP12237 AiE0452h SYFP2 DRD2 Medium spiny neurons (D2-MSN) Striatum 199777 pAAV-AiE0452h-minBG-iCre...(R297T)-BGHpA AiP13781 AiE0452h Cre DRD2 Medium spiny neurons (D2-MSN) Striatum 230871 pAAV-AiE0452h-minBG-FlpO-WPRE3...

  9. Brain Armamentarium

    Type
    Collection
    ...CN4825) Expression of iCre(R297T) in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru 214858-PHPeB...of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru...Expression of jGCaMP8s in striatal direct pathway medium spiny neurons (D1 MSNs) Jonathan Ting Viviana Gradinaru... CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru...of CoChR-EGFP in striatal direct pathway medium spiny neurons (D1 MSNs) Jonathan Ting Viviana Gradinaru...of jRGECO1a in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru...for Cre recombinase expression in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru 199774-PHPeB...

  10. Genetic Code Expansion

    Type
    Collection
    ... synthetase M. mazei Mammalian TAG Ivana Nikić-Spiegel 182537 pEVOL-MmAcKRS1-PylTUUA PylRS M. mazei Bacterial...NESPylRS(AF) PylRS M. mazei Mammalian TAG Ivana Nikić-Spiegel 182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_... synthetase M. mazei Mammalian TAG Ivana Nikić-Spiegel 182881 Mut1-RS tyrosyl-tRNA synthetase M. jannaschii...

  11. Neurodegeneration Research Collection

    Type
    Collection
    ... upper and lower motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads ...brain. The cause of this neuron death is unknown. Despite the idiopathic (having no specific known cause)...

  12. Plasmids for Stem Cell Research

    Type
    Collection
    ... of mouse and human fibroblasts into functional spinal motor neurons. Cell Stem Cell. 2011 Sep 2;9(3):...2013 May;31(5):426-33. Tesar Fibroblasts Medium Spiny Neurons Lentiviral Human Generation of Human Striatal...

  13. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Serotonin (5-HT) sensor psychLight Psychedelic-inspired drug discovery using an engineered biosensor. ...glutamate receptor Glutamate receptor exocytosis and spine enlargement during chemically induced long-term ...

  15. Recombinases AAV Preps

    Type
    Collection
    ...pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE EF1a EGFP 8 Ivo Spiegel 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP none 5, PHPeB...

  16. p53 Pathway

    Type
    Collection
    ...growth factor binding protein 3 KAI CD82 molecule Maspin Serpin family B member 5 MDM2 MDM2 proto-oncogene...

  17. TALEN Guide

    Type
    Collection
    ...Dr. Keith Joung’s lab at Massachusetts General Hospital also recently released a TALEN kit through Addgene...

  18. AAV Molecular Tools

    Type
    Collection
    ...and mScarlet-KASH for silencing neurons. 8 Ivo Spiegel 200777 AAV-HAPLN1 hSyn-driven, constitutive Expresses...

  19. CRISPR References and Information

    Type
    Collection
    ...in Japan. Developed by the Kumiko Ui-Tei Lab . CRISPick (Link opens in a new window) Ranks and picks candidate...

  21. Plan Your Experiment

    Type
    Collection
    ...transgene expression with AAVs can be long-lived, despite recombinant AAVs not integrating into the host ...

  22. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... pThr11 Rat Mouse IgG2a 206530 Anti-Haspin/GSG2 [N128A/2R] Haspin/GSG2 Human Mouse IgG2a 206531 Anti-MBP...

  23. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ... Other variants of Spinach include Baby Spinach, a miniaturized version of Spinach that retains similar... Hammond’s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified...was consequently brighter than Spinach. The initial studies using Spinach2 showed its ability to track RNA...imaging. Bunch of baby spinach, or BoBS, contains tandem repeats of Baby Spinach. It was developed in a...twice as bright as Spinach2 when bound to DFHBI. It’s also smaller than any form of Spinach - only 49 nucleotides...Addgene’s collection. Green fluorescent aptamers Spinach and Broccoli and their derivatives are aptamers..., but not quantitative, studies. Additionally, Spinach, the first aptamer identified to bind DFHBI, is...

  24. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...other labs. Spike protein, variants, and pseudotyping The SARS-CoV-2 virus depends on its spike protein to...easily study the spike variants, scientists have developed ways to pseudotype the spike protein onto another...This version of the spike protein was developed based on 100 structure-guided spike designs and has improved...for pseudotyping lentivirus with the SARS-CoV-2 spike protein. They deposited the plasmids used for this...Addgene which include a C-terminal deletion in the spike protein to improve expression and pseudotyping efficiency...individuals (Tong et al., 2021). Another version of the spike protein, called HexoPro, was developed by Jason ...improved protein yields and stability. This stabilized spike protein can be used for the development of vaccines...

  25. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...first segments of the spinal cord showing the cortico spinal tract, CST, spinal cord and cranial nerve...Cuneate tract, dCST= dorsal cortico spinal tract, vCST= ventral cortico spinal tract, dc= decussation of the.... Pubmed. Resources: http://openspim.org/Welcome_to_the_OpenSPIM_Wiki http://www.janelia.org/lab/keller-lab...single-plane or selective-plane illumination microscope (SPIM). In order to improve axial resolution and reduce...cleared organs) method of imaging rodent brains and spinal cords by combining Ultramicroscopy with tetrahydrofuran-based...

  26. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...cytometry, compensation is the process of correcting spillover from one fluorescent channel to another. When ...fluorescent channels, you can correct for this spillover and more easily separate out your target population...another. This overlap in spectra is often called "spillover." For example, if your flow cytometer reads out...one cytometer and AmCyan on another.  What does spillover look like and how do I fix it? When plotting two... see a diagonal population, this is a sign of spillover and indicates that it is necessary to apply a ...therefore would say that APC is “bleeding,” or spilling over, into APC-Cy7 channel and needs to be compensated...perpendicular vertical line.     In general, most spillover between samples can be fixed with fairly low compensation...

  27. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...these devastating afflictions. Elia Di Schiavi - Spinal muscular atrophy Dr. Elia Di Schiavi is a Research...Bioresources in Naples, Italy. His group works on spinal muscular atrophy, a disease in which disruption...of the smn1 gene results in the degeneration of spinal cord motor neurons. This disease is estimated to...While disruption of the smn1 gene is known to cause spinal muscular atrophy, the molecular mechanisms behind...clinic and thereby restore motor neuron function in spinal muscular atrophy patients. Noushin Nabavi - Peritoneal...Systematic review of incidence and prevalence of spinal muscular atrophy (SMA)." European Journal of Paediatric...

  28. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...presence of that metabolite (Fig.2). Spinach eventually evolved into Spinach2, a more stable and brighter version...essentially mimicking GFP.  Though the aptamer, called Spinach, required longer exposure time than its protein...possibilities. For instance, further selection of Spinach with a target metabolite creates an aptamer that...also has a higher melting temperature than its Spinach ancestors, and has a lower magnesium dependence...Activity with GFP Resources at Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene...

  29. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...are administered in the cisterna magna or lumbar spine. Image and caption courtesy of Challis et al., 2022...: somatic and visceral sensory ganglia, type II spiral ganglion neurons Mice, rats No (mechanism unknown...MVD1A is effective in transducing brain cells and spinal cords of both C57BL/6J and BALB/cJ mice through...the brain, and a lower transduction rate in the spinal cord. Additionally, AAV.CAP-B10 is targeted away...used to target endothelial cells in the retina and spinal cord (Krolak et al., 2022). Systemic capsids for... neurons, with a lower transduction rate in the spinal cord. It is detargeted from the liver and shows...brain, except for the cerebellum, brain stem, and spinal cord. All PALs, including PAL1D, are detargeted...

  30. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...encodes the mutated spike protein and a barcode, and is designed so that the SARS-CoV-2 spike RBD is displayed...deployed around the world to combat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus... the receptor binding domain (RBD) of the viral spike protein.   Figure 1. (A) SARS-CoV-2 RBD DMS...converting enzyme 2 (ACE2) cell-surface receptor. The spike protein RBD responsible for binding ACE2 is the ...
Showing: 1 - 30 of 306 results