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  2. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ...SARS-CoV-2 spike variants. “We created a series of spike expressing plasmids which encode spikes found on... Figure 1: Neutralization levels against spike pseudovirus decreases depending on the variant. ...Garcia-Beltran et al., 2021 with permission.   The spike variant pseudovirus neutralization assay Balazs’..., 2021 with permission.   Mutations in the spike protein are one of the key drivers in determining...

  3. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...Huber S, Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA (2018... identified nanobodies that bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the ...enter host cells. To do this, they injected the spike protein from two different coronaviruses into a ...isolated special camelid antibodies that bind to the spike proteins in cell culture, and inhibit these viruses...generate sybodies, synthetic nanobodies against the spike protein (Walter et al., 2020). No llamas required...Receptor Binding Domain found on the membrane-bound spike protein, in a remarkable 12 working days. While ...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...PRKCG MTH Spinocerebellar ataxia 30 Frederic Mushinski 8418 pLTR PKC gamma PRKCG Spinocerebellar ataxia ...GFP CMV Spinocerebellar ataxia 27 Tobias Meyer 21204 GFP-N2-PKCgamma PRKCG GFP CMV Spinocerebellar ataxia...GFP CMV Spinocerebellar ataxia 25 Tobias Meyer 21215 GFP-C2gamma PRKCG GFP CMV Spinocerebellar ataxia ...CMV Spinocerebellar ataxia 23 Bernard Weinstein 21237 PKC gamma dNPS PRKCG HA CMV Spinocerebellar ataxia... CMV Spinocerebellar ataxia 21 Bernard Weinstein 21239 PKC gamma DN PRKCG HA CMV Spinocerebellar ataxia...CMV Spinocerebellar ataxia 15 Ryohei Yasuda 112270 CMV-PKCg-mEGFP-N1 PRKCG GFP CMV Spinocerebellar ataxia...GFP CMV Spinocerebellar Ataxia Richard Youle 176464 pVPS13D-I-EGFP VPS13D GFP CMV Spinocerebellar Ataxia...

  5. COVID-19 Resources

    Type
    Collection
    ...all spike expression plasmids in our collection that are suitable for generating SARS-CoV-2 spike pseudotyped...Libraries SARS-CoV-2 Spike (S) Ectodomain and RBD Libraries - Libraries of Spike (S) Ectodomain and RBD...SARS-CoV-2 Spike Receptor Binding Domain Deep Mutation Scanning Library - Library of Spike protein RBD...entry driven by the severe acute respiratory syndrome coronavirus spike protein. . PCP4 - Kogiso H, et ...SARS-CoV and MERS CoV, depends on binding of the viral spike (S) protein to cellular receptors. The cellular ...and more. Pooled Libraries : Pooled libraries of spike protein mutations. Research Articles : A list of...contains: Plasmids for E. coli and Yeast expression Spike protein constructs for making VSV pseudo virus Mammalian...

  6. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Pooled Library 188526 iSPI_pSer_Subpool#1 Pooled Library 188527 iSPI_pSer_Subpool#2 Pooled Library 188528...188528 iSPI_pSer_Subpool#3 Pooled Library 188529 iSPI_pSer_Subpool#4 Pooled Library 188530 iSPI_pSer_Subpool...Pooled Library 188531 iSPI_pSer_Subpool#6 Pooled Library 188532 iSPI_pSer_Subpool#7 Pooled Library 188533...188533 iSPI_pSer_Subpool#8 Pooled Library 188534 iSPI_pSer_Subpool#9 Pooled Library 188535 iSPI_pSer_Subpool...using iSPI, rEcoli XPS, and pSerOTS-C1* (V70) (ca. 2022) from the Rinehart lab: Methods for iSPI, rEcoli...XpS in the Methods section link below (Methods for iSPI, rEcoli XPS, pSerOTS-C1*). The synthetic human phosphoproteome... Iterative Synthetically Phosphorylated Isomers (iSPI). This new resource described in (Gassaway et al...

  7. Brain Armamentarium

    Type
    Collection
    ...CN4825) Expression of iCre(R297T) in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru 214858-PHPeB...of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru...Expression of jGCaMP8s in striatal direct pathway medium spiny neurons (D1 MSNs) Jonathan Ting Viviana Gradinaru... CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru...of CoChR-EGFP in striatal direct pathway medium spiny neurons (D1 MSNs) Jonathan Ting Viviana Gradinaru...of jRGECO1a in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru...for Cre recombinase expression in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru 199774-PHPeB...

  8. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...-BGHpA AiP13044 AiE0779m_3xC2 SYFP2 DRD1 Medium spiny neurons (D1-MSN) Striatum 199776 pAAV-AiE0779m_3xC2...R297T)-BGHpA AiP13779 AiE0779m_3xC2 Cre DRD1 Medium spiny neurons (D1-MSN) Striatum 214855 pAAV-AiE0779m_3xC2...minBG-FlpO-WPRE3-BGHpA AiP14201 AiE0779m_3xC2 FlpO DRD1 Medium spiny neurons (D1-MSN) Striatum 224206 pAAV-AiE0780m-minCMV-SYFP2...WPRE3-BGHpA AiP15827 AiE0780m SYFP2 DRD1 Medium spiny neurons (D1-MSN) Striatum 224200 pAAV-AiE0780m-minBG-iCre...(R297T)-BGHpA AiP15578 AiE0780m Cre DRD1 Medium spiny neurons (D1-MSN) Striatum 191707 pAAV-AiE0452h-minBG-SYFP2...WPRE3-BGHpA AiP12237 AiE0452h SYFP2 DRD2 Medium spiny neurons (D2-MSN) Striatum 199777 pAAV-AiE0452h-minBG-iCre...(R297T)-BGHpA AiP13781 AiE0452h Cre DRD2 Medium spiny neurons (D2-MSN) Striatum 230871 pAAV-AiE0452h-minBG-FlpO-WPRE3...

  9. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Plasmids Expressing Spike and Spike Variants Full-length wildtype SARS-CoV-2 spike protein is inefficient...plasmids for viral pseudotyping with SARS-CoV-2 spike protein. Collections...replication-defective virus particles with the SARS-CoV-2 spike (S) glycoprotein on the envelope surface. Pseudotyped...particles have been pseudotyped with the SARS-CoV-2 Spike protein and you will find examples of each in Addgene's.... Use the search bar to find a particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant...Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein Nemazee lab - Pseudotyped virus protocol...Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Addgene COVID-19 Resources and ...

  10. Neurodegeneration Research Collection

    Type
    Collection
    ... upper and lower motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads ...brain. The cause of this neuron death is unknown. Despite the idiopathic (having no specific known cause)...

  11. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... 7.3 for suspension cells. For adherent cells, aspirate media. Add 20 μl of 0.05% trypsin-EDTA to each...200 μl. Wait 24 - 72 hr to allow cells to grow. Aspirate media. Add 50 μl DNA extraction solution per well...

  12. Plasmids for Stem Cell Research

    Type
    Collection
    ... of mouse and human fibroblasts into functional spinal motor neurons. Cell Stem Cell. 2011 Sep 2;9(3):...2013 May;31(5):426-33. Tesar Fibroblasts Medium Spiny Neurons Lentiviral Human Generation of Human Striatal...

  13. Genetic Code Expansion

    Type
    Collection
    ... synthetase M. mazei Mammalian TAG Ivana Nikić-Spiegel 182537 pEVOL-MmAcKRS1-PylTUUA PylRS M. mazei Bacterial... synthetase M. mazei Mammalian TAG Ivana Nikić-Spiegel 182881 Mut1-RS tyrosyl-tRNA synthetase M. jannaschii...

  14. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Serotonin (5-HT) sensor psychLight Psychedelic-inspired drug discovery using an engineered biosensor. ...glutamate receptor Glutamate receptor exocytosis and spine enlargement during chemically induced long-term ...

  16. Adenovirus Plasmids

    Type
    Collection
    ...double-stranded DNA viruses known to cause mild to severe respiratory disease in humans. Advantages of adenoviruses...

  17. p53 Pathway

    Type
    Collection
    ...growth factor binding protein 3 KAI CD82 molecule Maspin Serpin family B member 5 MDM2 MDM2 proto-oncogene...

  18. TALEN Guide

    Type
    Collection
    ...Dr. Keith Joung’s lab at Massachusetts General Hospital also recently released a TALEN kit through Addgene...

  19. Viral Vectors

    Type
    Collection
    ...of our viral pages, we thank Boston Children's Hospital Viral Core . Viral Plasmid Biosafety Resources...

  20. Plan Your Experiment

    Type
    Collection
    ...containing a C nucleotide at the same position in spite of being a perfect match for the target sequence...

  22. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... pThr11 Rat Mouse IgG2a 206530 Anti-Haspin/GSG2 [N128A/2R] Haspin/GSG2 Human Mouse IgG2a 206531 Anti-MBP...

  23. Cre-lox system

    Type
    Collection
    ...85797 pSkipFlox split Cre Pfcrt P. falxiparum Spielmann 86641 pLenti-hSynapsin-Cre-WPRE Cre hSyn Lentiviral...

  24. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ... Other variants of Spinach include Baby Spinach, a miniaturized version of Spinach that retains similar... Hammond’s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified...was consequently brighter than Spinach. The initial studies using Spinach2 showed its ability to track RNA...imaging. Bunch of baby spinach, or BoBS, contains tandem repeats of Baby Spinach. It was developed in a...twice as bright as Spinach2 when bound to DFHBI. It’s also smaller than any form of Spinach - only 49 nucleotides...Addgene’s collection. Green fluorescent aptamers Spinach and Broccoli and their derivatives are aptamers..., but not quantitative, studies. Additionally, Spinach, the first aptamer identified to bind DFHBI, is...

  25. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...other labs. Spike protein, variants, and pseudotyping The SARS-CoV-2 virus depends on its spike protein to...easily study the spike variants, scientists have developed ways to pseudotype the spike protein onto another...This version of the spike protein was developed based on 100 structure-guided spike designs and has improved...for pseudotyping lentivirus with the SARS-CoV-2 spike protein. They deposited the plasmids used for this...Addgene which include a C-terminal deletion in the spike protein to improve expression and pseudotyping efficiency...individuals (Tong et al., 2021). Another version of the spike protein, called HexoPro, was developed by Jason ...improved protein yields and stability. This stabilized spike protein can be used for the development of vaccines...

  26. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...first segments of the spinal cord showing the cortico spinal tract, CST, spinal cord and cranial nerve...Cuneate tract, dCST= dorsal cortico spinal tract, vCST= ventral cortico spinal tract, dc= decussation of the.... Pubmed. Resources: http://openspim.org/Welcome_to_the_OpenSPIM_Wiki http://www.janelia.org/lab/keller-lab...single-plane or selective-plane illumination microscope (SPIM). In order to improve axial resolution and reduce...cleared organs) method of imaging rodent brains and spinal cords by combining Ultramicroscopy with tetrahydrofuran-based...

  27. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...cytometry, compensation is the process of correcting spillover from one fluorescent channel to another. When ...fluorescent channels, you can correct for this spillover and more easily separate out your target population...another. This overlap in spectra is often called "spillover." For example, if your flow cytometer reads out...one cytometer and AmCyan on another.  What does spillover look like and how do I fix it? When plotting two... see a diagonal population, this is a sign of spillover and indicates that it is necessary to apply a ...therefore would say that APC is “bleeding,” or spilling over, into APC-Cy7 channel and needs to be compensated...perpendicular vertical line.     In general, most spillover between samples can be fixed with fairly low compensation...

  28. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...these devastating afflictions. Elia Di Schiavi - Spinal muscular atrophy Dr. Elia Di Schiavi is a Research...Bioresources in Naples, Italy. His group works on spinal muscular atrophy, a disease in which disruption...of the smn1 gene results in the degeneration of spinal cord motor neurons. This disease is estimated to...While disruption of the smn1 gene is known to cause spinal muscular atrophy, the molecular mechanisms behind...clinic and thereby restore motor neuron function in spinal muscular atrophy patients. Noushin Nabavi - Peritoneal...Systematic review of incidence and prevalence of spinal muscular atrophy (SMA)." European Journal of Paediatric...

  29. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...presence of that metabolite (Fig.2). Spinach eventually evolved into Spinach2, a more stable and brighter version...essentially mimicking GFP.  Though the aptamer, called Spinach, required longer exposure time than its protein...possibilities. For instance, further selection of Spinach with a target metabolite creates an aptamer that...also has a higher melting temperature than its Spinach ancestors, and has a lower magnesium dependence...Activity with GFP Resources at Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene...

  30. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...are administered in the cisterna magna or lumbar spine. Image and caption courtesy of Challis et al., 2022...: somatic and visceral sensory ganglia, type II spiral ganglion neurons Mice, rats No (mechanism unknown...MVD1A is effective in transducing brain cells and spinal cords of both C57BL/6J and BALB/cJ mice through...the brain, and a lower transduction rate in the spinal cord. Additionally, AAV.CAP-B10 is targeted away...used to target endothelial cells in the retina and spinal cord (Krolak et al., 2022). Systemic capsids for... neurons, with a lower transduction rate in the spinal cord. It is detargeted from the liver and shows...brain, except for the cerebellum, brain stem, and spinal cord. All PALs, including PAL1D, are detargeted...
Showing: 1 - 30 of 294 results