... CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn Calcium sensor...pGP-AAV-syn-jGCaMP7f-WPRE Syn Calcium sensor GCaMP Kim , GENIE 135632 pAAV-S5E2-GCaMP6f E2 regulatory element Calcium sensor ...pAAV.Syn.NES-jRGECO1a.WPRE.SV40 Syn Calcium sensor RGECO Kim , GENIE 162375 pGP-AAV-syn-jGCaMP8m-WPRE Syn Calcium sensor GCaMP...Viviana Gradinaru's lab at Caltech. Viral... Viral Vector Packaging Service AAV Caltech Systemic Capsids Viral Vector...Vector Packaging Service: Caltech Systemic Capsids PHP.eB PHP.S PHP.V1 MaCPNS1 MaCPNS2 CAP-B10 CAP-B22... from the Viviana Gradinaru laboratory and the Caltech CLOVER Center (Link opens in a new window) . As...

...peptides with known subcellular localization patterns. Useful for co-localization studies....mEmerald-Paxillin-22 Focal Adhesions paxillin mEmerald* Michael Davidson 15233 Paxillin-pEGFP Focal Adhesions paxillin... RFP-zyxin Focal Adhesions Zyxin RFP Anna Huttenlocher 11908 pEGFP-N1 alpha-actinin 1 Focal Adhesions ...pPaxillin-PSmOrange Focal Adhesions paxillin PSmOrange Vladislav Verkhusha 79999 pZyxin-miRFP703 Focal Adhesions...Dorus Gadella 158004 pCMV-mGold-Calnexin-N-14 Endoplasmic Reticulum Calnexin mGold Francois St-Pierre 158002...Organelle Markers Fluorescent Proteins: Subcellular Localization More Fluorescent Protein Resources: Empty Backbones...Secretory Pathway Background The subcellular localization of a protein is often tied to its function, ...

...I’ve answered hundreds of phone calls and thousands of customer service emails in my six years as a senior...spend far less time answering help emails and phone calls, I feel compelled to share my years of accumulated...customer, can get the most out of your email or phone call. Though my experience is based solely on my time...texting, actually writing an e-mail or making a phone call is a big commitment when you’re busy. Your time ... A lot of companies and organizations selling biological reagents have a ton of data on the catalog page...it’s own tongue-in-cheek website.  If you are skeptical about using Google for serious science questions...thread or remove a good 5 minutes from our phone call. If your concern is based on data you have produced...

...source of information on recent advances in the biological sciences and techniques that simplify and expedite... help hone your blog post for our audience of biological scientists from a variety of different fields...

...Chavez, researchers at the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 is an enormously...reassemble when exposed to specific UV wavelengths or chemicals (Polstein and Gersbach, 2015; Zetsche et al., ...much cheaper to use Cas9 activators. This larger scale of activation allows for exploratory-based research...pathways and genetic interactions. Additionally, large scale CRISPR-based screens can be used to gain insight... Tuttle and Alex Chavez! Marcelle Tuttle is a medical student at Tufts University and former researcher...researcher at the Wyss Institute for Biologically Inspired Engineering.     Alejandro (Alex) Chavez is a member...MC, Qi LS, Kampmann M, Weissman JS (2014) Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation...

... use a mechanical method before your enzyme lysis. For cell cultures, enzyme lysis is typically sufficient...for your experiment. But if you understand the technical aspects of a western blot, you'll be able to understand... so let's dive in!      Figure 1: The technical decisions to make when designing a western blot...multiplexing is challenging and limited. Direct westerns typically rely on primary antibodies conjugated to fluorescent... antibody or loading sample, which may not be practical. Conjugated primary antibodies can be more difficult...lengthy protocol, require the use of hazardous chemicals, and are not always consistent. Precast gels, ...unless you buy specialty nitrocellulose membranes specifically developed to allow stripping. And as a safety...

...newly developed calcium sensors. Selecting a calcium sensor Genetically encoded calcium indicators (GECIs...biosensors. Nature chemical biology. 2021 May;17(5):509-18. Inoue M. Genetically encoded calcium indicators to...Iino M. Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical journal...fluorescent proteins linked to calcium binding proteins (e.g., calmodulin or troponin C). Their fluorescent... monitor calcium concentrations and transients in living cells and organisms. Unlike chemical indicators...unusually fast calcium dynamics, whereas non-neuronal systems tend to have slower, but larger, calcium changes...sensitivity parameters. Sensitivity and Localization The calcium concentrations in different subcellular...

...values – numerical risk allowance in a statistical test. Distribution - spread of data; typically, normal...Statistics – The basics Statistical analysis is what determines if there is statistical significance among ...are statistically different. They are classic parametric tests and one of the simplest statistical tests...if the dependent variables worked synergistically, antagonistically, were additive, etc.). ANCOVA and...analysis can provide information on specifically which groups are statistically different within your ANOVA/ANCOVA... data in ascending numerical value relative to quantiles derived from a theoretical distribution. If your... non-parametric statistical tests! Adding it all up  There are many, many statistical tests beyond the...

...names for technical support roles. Examples are Technical Service Representative, Technical Support Specialist...these departments from technical support. Technical support careers in brief Technical support careers are...troubleshoot problems at the bench. Specifically, roles in technical support make excellent use of the ...worked as a technical support specialist for 4 years. Read on for insights from my time in technical support...products successfully. In technical support you may have to answer technical enquiries such as: “Can I... areas of expertise in technical support positions. When applying for technical support positions, you...familiarize yourself with technical support. Even having personal experience with technical support through the...

... the types of viruses used as biological tools.   Viruses as biological tools In nature, viruses infect... why viruses are such wonderful scientific and medical tools. As scientists, we can manipulate what this...effectively infect a wide range of cell types that are typically ‘difficult to transfect’, such as primary cells...with whole organisms. Finally, viral delivery is typically straightforward across applications, while electroporation... cells. They accomplish this through nuclear localization signals which can traffic their viral particles...Packaging Capacity (~4.5kb From basic science to clinical applications, viruses are true bench to bedside...

...re working on a project where we aim to do large scale DNA preps with multiple samples without cross contamination...

...packaging, considering using this PEI Calculator. AAV packaging typically requires transfecting three plasmids... ratios right, you need to do a few calculations. This calculator can do the math for you and it’s simple... download the PEI calculator for AAV packaging Use the PEI transfection calculator for AAV packaging ...spreadsheet will calculate the following μg of each plasmid needed per dish or stack. Calculations are based...corresponding column in the calculator spreadsheet: We hope that this calculator will make it easier to plan...whole experiment And here’s the equation the calculator uses to give you the μl needed of each plasmid...experiments. Let us know what you think about the calculator in the comments section below. References 1....

...background, which means that each mouse is genetically identical and all mice of the strain are homozygous...create a limited but genetically diverse gene pool. Outbred strains are strategically bred to maintain maximum...using the same nuclease may not result in genetically identical mice. Thus, scientists must genotype each...organism used to understand mammalian traits and genetically engineered mouse models provide researchers with...useful and adaptable tools to perform basic and preclinical research. For scientists new to using mouse models...) National Institutes of Health (NIH) Swiss. Genetically engineered mice Mice can be engineered by inserting...into a blastocyst. There are several types of genetically engineered mice: transgenic mice, knockout mice...

...SPARC to image the calcium response in the dendrites of T5 neurons by placing the calcium indicator GCaMP6f...researchers to label just a fraction of a single genetically-defined subset of cells. By labeling fewer cells...While transgenic animals are commonly used to specifically manipulate a cell type of interest in an organism...Drosophila require heat-shocking the system or using chemical inducers of gene expression (del Valle Rodríguez...cells are not labeled. PhiC31, a recombinase. It specifically integrates an attP site into an attB site. In...intermediate (d), and sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe...GCaMP6f in the effector position, as well as to optogenetically control the depolarization of R2 neurons using...

...Here, we will explain how practical brightness is determined and why practical brightness is a better criterion...criterion than theoretical brightness for selecting the brightest fluorescent protein. Theoretical brightness...quantum yield (QY) respectively. The theoretical brightness is calculated by multiplying EC by QY (EC*QY, ...higher the number, the higher the theoretical brightness. The theoretical brightness often appears in tables...proteins based on their practical brightness in your experimental set up. The practical brightness takes all...same reference protein) under identical conditions, a ranking of the practical brightness can be made (Goedhart...which is a key determinant of the practical brightness. Therefore, practical brightness provides a better ...

...expected when we originally sent out the call for applications. Cervical cancer screening in Ghana Gladys is... she can use as positive controls in PCR-based cervical cancer screens. This third grant represents the...is currently working to enhance cervical cancer screening in Ghana. While pap smears and visual tests ...Papillomavirus (HPV). As Gladys wrote in her application, “Cervical Cancer is the leading cause of cancer related ...in other developing countries” and “Almost all cervical cancers are caused by persistent infection of ...controls To start her work, Gladys will improve cervical cancer screening for a limited group of Ghanaian...including PCR screening for HR HPV alongside the cytological tests currently in use. Gladys will use plasmids...

...about Julie Cicalese  Ms. Cicalese served as the Chief Human Resources Officer for Harvard Medical School ...excited to welcome Julie Cicalese to the Addgene Board of Directors. Cicalese served as the Chief Human...Human Resource Officer for Harvard Medical School. The addition of Cicalese to the Board of Directors brings...When I was working in Human Resources at Harvard Medical School, Joanna was my colleague. She mentioned ... science but my prior HR experience at Harvard Medical School means I understand the needs of scientists...Resources Association, Association of American Medical Colleges and the Society for Human Resource Management...

...Addgene can be found on our Contact page. You’ve called. You’ve emailed. And Addgene has been there to ...plasmid questions. But until now, you’ve always had to call the United States - Cambridge, MA to be exact. Today...collaboration with LGC Standards. And now you’ll be able to call us - Benoit Giquel and Emma Markham - the newest...the University of London and the University of California, specialising in Molecular Biology. She has worked...Genetics. Since then she has worked in the the UK, California, Africa, and Australia, viewing science as a ...   Europe, we are all geared up to answer your calls, emails, or maybe even visit your lab! We look forward...

...recent paper from Ed Callaway’s lab (Fischer, KB. 2019) set-out to systematically test the potential causes... was not present (Kuhlman, SK. 2008). While theoretically this strategy should work, these vectors suffered...expression.  As such, researchers developed new tools called FLEx/DIO vectors where the transgene is floxed ...conditions used to minimize recombination that are typically employed during viral plasmid amplification (Fischer... the researcher's system before beginning large scale experiments. This will help to ensure the accuracy...doi.org/10.1128/jvi.02213-17 Fischer KB, Collins HK, Callaway EM (2019) Sources of off-target expression from...IR (2020) Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations. Front Synaptic...

... you to do customized genetic modification on a scale that was really not possible before,” said the Broad... matter of a few days. Their library, which they call GeCKO, targets 18,080 human genes with 64,751 unique...aimed for 300 cells per construct, but in some biological systems one “might need larger numbers.” Further...Lander ES. Science. 2013 Dec 12. (PubMed). Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells. Shalem...

...opportunity to study it and see what happens when technological change happens really fast,” Thompson says. ... groups have generated robust tools that have practical applications in monitoring the accidental or intentional...essential and complementary tools in a time where biological engineering and synthetic biology is expanding...

...while the wet-lab resources, like constructs or biological material, can be deposited in the specific repositories...

...an experimental system comes with fewer ethical and logistical challenges than does working with animals...grown to include local school districts, professional education associations, and local organizations such...Education & Outreach Specialist for the Arabidopsis Biological Resource Center and the Center for Applied Plant...University. Established in 1991, the Arabidopsis Biological Resource Center (ABRC) is one of two global stock...preference for using animal systems to demonstrate biological concepts - and you have yourself a plant publicity...publicity crisis. The Arabidopsis Biological Resource Center (ABRC) set out to remedy that with the establishment...conducting teacher workshops, or staffing a booth at local science festivals, ABRC gets the word out about ...

...activate essentially any gene,” she says. The technical leap from mutating and deactivating a gene or ...Zhang’s lab describe just such a system, which they call CRISPR/Cas9 Synergistic Activation Mediator or SAM... and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating...distinct effector domains that work together synergistically, modeled after natural transcription activation...GeCKO, a lentiviral CRISPR library enabling genome-scale, knockout screening). As the Zhang lab's Nature ... is robust, specific, and can facilitate genome-scale gain-of-function screening when combined with a ...demonstrated in a paper in Cell that they could typically achieve 90-99% knockdown with minimal off-target...

...specific and much faster than any genetic or pharmacological manipulations on cells, and has potential use... platform for visualizing RNA production and localization Article contributed by Tyler Ford  Listen to...set of tools that can be used to visualize RNA localization in mammalian cells. These tools consist of cobalamin-binding...platform at Addgene. Braselmann E, et al. Nature Chemical Biology. 2018 PubMed PMID: 30061719. New tools...expression vector (pRSETB). Fabritius A, et al. Cell Chemical Biology 2018. PubMed: PMID 30220597. ACE system...

...reporter for calcineurin activity in yeast Article contributed by Leah Schwiesow Calcineurin is a protein...intracellular calcium levels within the cell. When levels of Ca2+ are high in the cell, calcineurin dephosphorylates...attractive drug target. Calcineurin reporters allow scientists to measure calcineurin dynamics and can serve...identify new antifungals that target calcineurin. Current calcineurin gene reporters are based on the bacterial...screening, calcineurin dynamics measurements, or monitoring in vivo. To overcome these technical issues, ...genetic tool for labeling just a fraction of a genetically-defined subset of cells. By labeling fewer cells...intermediate (d), and sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe...

...Gordon Fishell Calcium GCaMP5 genetically encoded calcium sensor Optimization of a GCaMP calcium indicator ... Javier García-Sancho Calcium Genetically encoded calcium indicators for optical imaging (GECOs), including...Schreiter Calcium GCEPIA1-SNAP ER for sensing ER calcium A genetically encoded ratiometric calcium sensor...Target Description Article PI Calcium jGCaMP8 Fast Genetically Encoded Calcium Indicators (Constitutive or... Loren Looger , GENIE Project Calcium GCaMP3 genetically encoded calcium sensor Imaging neural activity...GCaMP calcium indicators. Nat Methods. 2009 Dec;6(12):875-81. Loren Looger Calcium NEMO calcium sensors...intracellular calcium. Nat Commun. 2021 Dec 9;12(1):7159. Dorus Gadella Calcium Teal genetically encoded calcium...

...-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Ting 51083 AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato...AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium sensor Ting 83899 pAAV-mDlx-GCaMP6f-Fishell...element Calcium sensor Fishell 51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate...jRGECO1a Calcium Sensor Kim , GENIE 100854 pAAV.Syn.NES-jRGECO1a.WPRE.SV40 Syn jRGECO1a Calcium Sensor ...pGP-AAV-syn-jGCaMP7s-WPRE Syn GCaMP7s Calcium sensor Kim 104489 pGP-AAV-syn-jGCaMP7b-WPRE Syn GCaMP7b Calcium sensor Kim 104491...Cre-dependent Calcium sensor Kim 104493 pGP-AAV-syn-FLEX-jGCaMP7b-WPRE Syn GCaMP7b, Cre-dependent Calcium sensor...Cre-dependent Calcium sensor Kim 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG GCaMP7f, Cre-dependent Calcium sensor...

...dendrite localization tag) Lin Tian 135420-AAV1 pAAV-syn-FLEX-jYCaMP1s Yellow protein calcium sensor expressed...pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P... pAAV-hSyn-JEDI-2P-Kv-WPRE Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P... application of innovative tools enabling large-scale real-time interrogation and monitoring of brain ...pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer Viviana ...

...antibody’s constant region - specifically, it is determined by a region called the “heavy chain constant ...reagents are molecular tools used in the lab to specifically and tightly bind to other proteins. Antibodies...interest. The protein that an antibody recognizes is called an antigen, while the specific binding site is ...referred to as an epitope. Production Antibodies are typically produced in one of three different methods: in...independently manipulated. Antibodies produced this way are called recombinant antibodies. Recombinant antibodies ...are responsible for binding the antigen, and are called the antigen binding fragments (Fab). The tips of...the Y structure and the lower part of the arms is called the constant region, and is shown in dark blue ...

...Anti-CALB2/calretinin [L122/68R-2b] CALB2/calretinin Human Mouse IgG2b 206642 Anti-CALB2/calretinin [L122...Anti-CALB2/calretinin [L122/68R-1] CALB2/calretinin Human Mouse IgG2a 206691 Anti-CALB2/calretinin [L122...Anti-Calbindin [L109/39R] Calbindin Human Mouse IgG2a 177455 Anti-Calbindin [L109/57R] Calbindin Human...Anti-Calretinin [L122/68R] Calretinin Human Mouse IgG2a 177469 Anti-Calretinin [L122/6R] Calretinin Human...Human Mouse IgG2b 206635 Anti-CALB1/calbindin [L109/39R-2b] CALB1/calbindin Human Mouse IgG2b 206636 Anti-Homer1L...Human Mouse IgG1 206686 Anti-CALB1/calbindin [L109/39R-1] CALB1/calbindin Human Mouse IgG1 206687 Anti-Homer1L...220384 Calretinin [L122/110R] Calretinin Human Mouse IgG2a 220385 Calretinin [L122/117R] Calretinin Human...

..., including calcium sensors, under the control of various promoters. Sensor Green Calcium Sensors GCaMP8...soma-GCaMP6 Twitch2B Yellow Calcium Sensors jYCaMP1 axon-jYCaMP1 Red Calcium Sensors CaMPARI jRCaMP1 jRGECO1...viral service encoding biosensor tools, including calcium sensors. Viral...jRGECO1 sRGECO Far-Red/NIR Calcium Sensors HaloCaMP1a HaloCaMP1b NIR-GECO Acetylcholine Sensors iAChSnFR..._ACh3.0 Syn GRAB_ACh3.0 none Constitutive 9 Li Calcium Sensor: GCaMP8f 162376 pGP-AAV-syn-jGCaMP8f-WPRE...CAG jGCaMP8f none Constitutive 1, 9, rg* Looger Calcium Sensor: GCaMP8s 162374 pGP-AAV-syn-jGCaMP8s-WPRE...CAG jGCaMP8s none Constitutive 1, 9, rg* Looger Calcium Sensor: GCaMP8m 162375 pGP-AAV-syn-jGCaMP8m-WPRE...

... of identical bases. Browse Plasmids: Prime Edit Large Scale Edits CRISPR can generate large-scale genetic...takes place during the S and G2 phases. Chemically or genetically inhibiting genes involved in NHEJ or upregulating...properties, often called a reeling mechanism. The targeted strand is cleaved non-specifically by Cas3 along...immunoprecipitation (enChIP) using CRISPR. Biochemical and Biophysical Research Communications . 439 (1), 132... generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR perfect...gRNAs will be expressed in the same cell. This is called multiplexing , and increases the likelihood that...DNA with no homology elsewhere in the genome. Realistically, most gRNA targeting sequences will have additional...

... beta CALC2, CGRP-II, CGRP2, FLJ30166 CALCR calcitonin receptor CRT, CTR, CTR1 CALCRL calcitonin receptor-like...CD88 CALCA calcitonin-related polypeptide alpha CALC1, CGRP, CGRP-I, CGRP1, CT, KC, MGC126648 CALCB calcitonin-related...beta-2-microglobulin - CALR calreticulin CRT, FLJ26680, RO, SSA, cC1qR CANX calnexin CNX, FLJ26570, IP90,...formerly 2B), catalytic subunit, alpha isoform CALN, CALNA, CALNA1, CCN1, CNA1, PPP2B PPP3CB protein phosphatase...guanyl releasing protein 1 (calcium and DAG-regulated) CALDAG-GEFI, CALDAG-GEFII, MGC129998, MGC129999...

...mitochondrial localization 101124 pSNAPf-H2B Control Plasmid SNAP-tag Control for nuclear localization 101123...mitochondrial localization 101125 pCLIPf-NK1R Control Plasmid CLIP-tag Control for cell surface localization 101127...surface localization 101131 pACP-GPI Control Plasmid ACP/MCP-tag Control for cell surface localization 101128...Control plasmids with a recognizable sub-cellular localization (membrane, nucleus, etc.) are also available...-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to SNAP-tag but reacts with benzylcytosine...visualization of complementary protein function or localization. ACP and MCP tags ACP- and MCP-tags are small...SNAP-tag Control for endomembrane (CaaX box) localization 101129 pSNAPf-Cox8A Control Plasmid SNAP-tag...

...interacting domain (NID) a nuclear localization signal (not essential for localization) the C - t erminal D omain... opens in a new window) ClinicalTrials.gov - a list of Rett syndrome clinical trials (Link opens in a ...window) PMID: 16615965 Leonard et al. 2017. Clinical and biological progress over 50 years in Rett syndrome...months of age. A defining feature, with onset typically between 18 to 30 months, is regression of previously...of Rett syndrome is currently based on specific clinical diagnostic criteria, defined as the presence of...MECP2 gene. Causative mutations for Rett syndrome typically arise spontaneously, although in rare cases Rett...of MECP2 is to regulate gene expression either locally or globally. The MECP2 protein contains several...

...collection contain fluorescent protein tags whose localization you can monitor in live cells (beware of artifacts...staining, and RNA transcript tags to monitor RNA localization. Using these plasmids you’ll hopefully get a...coat protein binding sites Philippe Cluzel RNA localization: Contains a 2x array of the binding sequence... that found in pZS*12-MS2-GFP ) to track RNA localization of your gene of interest once its appropriate... inducible) MBP, 6x His, signal sequence for localization to the periplasm David Waugh Empty lactose/IPTG...

...small biomolecules or other physiological intracellular processes with genetically encoded fluorescent biosensors...mammals. Regulate Biological Activity Use fluorescent proteins to modulate biological activity, like transcription...Plasmids by Category Empty Backbones Understand the localization and/or function of your protein of interest ...Plasmids | Guide Use small molecules to activate genetically engineered cellular receptors that affect signalling...signalling pathways within cells. Subcellular Localization Determine where your protein of interest resides...Resources FPbase from Talley Lambert at Harvard Medical School Fluorescent Biosensor Database designed ...

...Yale have made improvements to this system by genomically recoding E.coli (Lajoie et al., Science 2013)...of the popular SepOTSλ ( 68292 ). An important technical note: pSerOTS-C1* ( 188537 ) is a ROP minus plasmid...of interest by mass spectrometry in a technique called serine-oriented library/kinase-library reactions...Barber et al, Biochemistry 2018). Iterative Synthetically Phosphorylated Isomers (iSPI). This new resource...proteomics search algorithms, proteomics phosphosite localization algorithms, or any method confounded by phosphosite...WW2 domain) References & Protocols Iterative Synthetically Phosphorylated Isomers (iSPI) described in: ...: A multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics. Gassaway...

...engineering, offering flexibility and easy multiplexing/scaling. Prokaryotes have long utilized CRISPR as a powerful...to destroy the phages upon re-exposure. More specifically, specialized Cas proteins snip foreign DNA into...editing with CRISPR is not only simple, but also scalable. Multiple gRNAs targeting multiple loci can be...robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology development has occurred...programmable platform well-suited for various biological and translational technologies. Biotechnology...agricultural and therapeutic applications. Despite the ethical controversies surrounding non-research applications... it’s adaptable to almost any model system or biological topic, and you don’t need to be an expert to ...