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We narrowed to 237 results for: Mammalian expression plasmids

Showing: 181 - 200 of 237 results

  1. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...CRISPR plasmids for transcriptional inhibition. Plasmids are available for expression in mammalian systems...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...of the coding sequence. If the plasmid you’re using does not also express a gRNA, you will need to use ...use a separate gRNA expression plasmid to target the dCas9-repressor to your specific locus. Want more information...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids for transcriptional repression of target genes. CRISPR...

  2. CRISPR Plasmids - Activate Gene Expression

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    Collection
    ...CRISPR plasmids for transcriptional activation. Plasmids are available for expression in mammalian systems...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...Activate CRISPR Plasmids: Activate Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick...your gene of interest. If the plasmid that you choose does not also express a gRNA, you will need to use...use a separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Want more ...Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter...

  3. New England Biolabs Cell-Imaging Plasmid Collection

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    Collection
    ...Cell-Imaging Plasmid Collection New England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental...for stable and transient mammalian expression 101133 pSNAP-CaaX Control Plasmid SNAP-tag Control for endomembrane...for stable and transient mammalian expression 101134 pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear...for stable and transient mammalian expression 101132 pMCP-GPI Control Plasmid ACP/MCP-tag Control for ...Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids for visualization...synthases needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7...Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B Control Plasmid SNAP-tag...

  4. TALEN Expression Vectors

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    Collection
    ...Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids This page lists plasmid vectors into ...same). All expression vectors listed below have the CMV promoter for mammalian cell expression and a T7 ...Mammalian expression vectors for TAL effector repeat arrays assembled using the REAL, REAL-Fast or FLASH... TALengineering Reagents TALEN Expression Vectors TALEN Expression Vectors for REAL, REAL-Fast and FLASH...REAL-Fast or FLASH methods) can be cloned for expression as TALENs. Additional details about the REAL,...promoter for in vitro transcription of RNA. TALENs expressed from these vectors also have a FLAG epitope tag...localization signal at their amino-terminus. The expression vectors listed below can be purchased together...

  5. CRISPR Plasmids - Purify Genomic Loci

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    Collection
    ...CRISPR plasmids to help purify genomic loci. Plasmids are available for expression in mammalian systems...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...Purify Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system...

  6. Mammalian RNAi Tools

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    Collection
    ... Plasmid Collections Mammalian RNAi Mammalian shRNA Tools for RNAi Browse Plasmids Resources ...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a ...retroviral and AAV backbone options. Plasmids for constitutive expression as well as those that allow for ... (Cre-lox) or inducible (Tet) expression are available. To find plasmids containing other RNAi components...mutations in therapeutic settings. As a tool in mammalian cell systems, silencing is achieved through the... occur via transfection or viral delivery of a plasmid. shRNAs are often delivered using viral vectors...

  7. CRISPR Plasmids - Cascade-Cas3

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    Collection
    ... CRISPR Cascade-Cas3 plasmids. Plasmids are available for expression in mammalian systems, bacteria, and... and plants. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert ...the Cascade complex to express equally. To combat this, some CRISPR-Cas3 plasmids take an all-in-one approach...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  8. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...CRISPR RNA targeting plasmids. Plasmids are available for expression in mammalian systems, bacteria, and...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ... that function in mammalian cells can be used to attentuate RNA levels. In mammalian systems, Cas13a does... and plants. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid... Plasmid Gene/Insert PI Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...

  9. Genomic Deletions in Mammalian Cell Lines

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    Collection
    ...Addgene plasmid ID 42230). This plasmid allows for the simultaneous expression of sgRNA and SpCas9, but...Genomic Deletions in Mammalian Cell Lines CRISPR: Protocol for Genomic Deletions in Mammalian Cell Lines Other...cloning and expression purposes. NOTE: Here we discuss the usage of the pSpCas9(BB) plasmid (pX330) (Addgene...utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP ...constructs in which multiple sgRNAs may be expressed from a single plasmid. Add “CACC” before the 20-mer guide... with a PCR cloning kit) into a plasmid vector. Transform the plasmid into DH5α E. coli cells and plate...Experiments (JoVE) about genomic deletions in mammalian cell lines using CRISPR/Cas9 CRISPR...

  10. Retrovirus Plasmids

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    Collection
    ... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG...Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope...envelope plasmid Nienhuis and Salmon 22501 pHEF-VSVG Envelope VSV-G-expressing envelope plasmid Kasparov Popular...γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want a retroviral plasmid with your gene...10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro for Puro... to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also expresses...

  11. CRISPR Plasmids - Base Edit

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    Collection
    ... CRISPR base editing plasmids. Plasmids are available for expression in mammalian systems, bacteria, plants...zebrafish. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break. CRISPR...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

  12. CRISPR Plasmids - dCas9-FokI

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    Collection
    ...CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila. Mammalian ID ...ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication...Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... be added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve...

  13. CRISPR Plasmids - CRISPR Transposases (CAST)

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    Collection
    ...Transposase plasmids. Plasmids are available for expression in mammalian systems and bacteria. Mammalian ID Plasmid...CRISPR Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base... Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Selectable...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... used in bacteria genome editing, though some mammalian systems have been developed. Figure 1: Overview...

  14. COVID-19 Resources

    Type
    Collection
    ...SARS-CoV Plasmids : Plasmids that are available containing SARS-CoV (SARS classic) sequences. Mammalian Targets...nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids Many plasmids containing SARS-CoV-2 sequences...distribute SARS-CoV-2 plasmids. The collection contains: Plasmids for E. coli and Yeast expression Spike protein...time. Return to top Plasmids Encoding Mammalian Genes or Inserts Several mammalian genes have been identified...a new window) BST-LF expression plasmid from Andrew Ellington's lab. Find plasmid tools that may be helpful...Addgene will maintain this plasmid collection page, which highlights plasmids available that may be useful...SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing...

  15. CRISPR Plasmids - Single-Strand Break (Nick)

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    Collection
    ...below for CRISPR nickase plasmids. Plasmids are available for expression in mammalian systems, bacteria, Drosophila...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR... CRISPR Nick CRISPR Plasmids: Nick Browse...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...

  16. Adenovirus Plasmids

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    Collection
    ... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources: Adenovirus...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral...viruses with multicistronic expression + Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are...homologous recombination of adenovirus plasmids in either mammalian cells or microorganisms, including bacteria...sequences from both plasmids. This DNA molecule can then be transfected into mammalian packaging cell lines...

  17. Plan Your Experiment

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    Collection
    ...dCas9 alone for mammalian cell lines Interfere Activate (CRISPRa) Increase gene expression without permanently...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ... your experiment) and the expression time (transient or stable). For plasmid and viral-based approaches...Delivery Using Plasmids The most straightforward method to deliver a CRISPR system into mammalian cells is ...is to directly transfect expression plasmids encoding your Cas protein and gRNA, which you generally perform...electroporation. Expression can be transient, or you can generate stable cell lines if your plasmid contains ...

  18. Antibody Production

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    Collection
    ...encoded by mammalian expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding...target antigen is first transiently expressed in mammalian cells. To learn which specific QC experiments were... low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified...encoding the heavy and light chains. Antibodies are expressed for 7 days and then purified from the supernatant...retained. If the target antigen is not endogenously-expressed (e.g., using an anti-GFP antibody), the target...tissue labeling pattern is compared to the mRNA expression pattern cited in the Tissue Atlas (Link opens...species-specific HRP-conjugated secondary antibody. Protein expression is compared to an untransfected control and the...

  19. Zhang Lab CRISPR Page

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    Collection
    ... SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...nuclei in addition to sgRNA expression. This plasmid contains two expression cassettes, Cre recombinase...., Science 2013). Additionally, we have a plasmid expressing SpCas9 alone without sgRNA. Full references...nickase) + single guide RNA: These plasmids contain two expression cassettes, a human codon-optimized ...sequence. 3. SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized SpCas9...

  20. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...CRISPR plasmids designed to introduce a DSB. Plasmids are available for expression in mammalian systems...Xenopus. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert... elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Xenopus ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids that create double strand breaks in DNA. CRISPR...
Showing: 181 - 200 of 237 results