We narrowed to 1,045 results for: STI;

...Content last reviewed: 9 December 2025 Do you have suggestions for other plasmids that should be added to this...

...Content last reviewed: 9 December 2025 Do you have suggestions for other plasmids that should be added to this...

...Promoter PI Publication Back to Top Do you have suggestions for other plasmids that should be added to this...

...viral expression but may still be sufficient for successful stimulation. Testing opsin expression  Regardless... of Antonello Bonci, Director of the National Institutes of Drug Abuse Intramural Research Program (NIH-NIDA...you want to use optogenetics The most important question to consider is what exactly do you want to use...optogenetics is best used to address a specific question. Optogenetics is a tool and, just like any other...circuit of interest in promoting reward is a self-stimulation experiment wherein mouse initiated optogenetic...in a reward sensation and subsequent repeated stimulation. A spatial memory task, on the other hand, would...opsin of choice in the brain region you wish to stimulate 2) Fabrication and implantation of the optical...

...Content last reviewed: 9 December 2025 Do you have suggestions for other plasmids that should be added to this...

...Promoter PI Publication Back to Top Do you have suggestions for other plasmids that should be added to this...

...ligated into Cas9 or dCas9-containing destination vectors. Destination vector options include humanized wt...Addgene. 8. Xie, Kabin, Minkenberg, Bastian, & Yinong Yang. “Boosting CRISPR/Cas9 multiplex editing capability...editing. Multiplexing gRNAs: the basics One common question Addgene Senior Scientists receive is: can I express...ordered assembly of multiple components into a destination vector. The first step in CRISPR/Cas9 Golden ...oligonucleotides specifying each gRNA target sequence into distinct expression vectors using the enzyme BbsI. These...together to allow for ordered assembly into a destination vector that expresses Cas9. This is illustrated...drive ordered assembly into a Cas9-containing destination vector. Note: depending on which method you use...

... where you can type in questions for the speaker. “I think a lot more questions get answered this way ...audience can help decide which questions get answered. “You can upvote a question and the host will see the...upvoted question.”If the Q&A session ran out of time, the speaker could continue to answer questions in the...thought of walking up to the mic and asking your question in a large conference room puts butterflies in...the highly voted questions,” says Zhang. “Usually the moderator starts by asking the speaker the highly...audience engaged. They first sent a pre-workshop questionnaire to the participants to learn about what they... when you are giving a research talk, there are still other ways to make your session more engaging, for...

...prepares one for numerous non-academic careers. Still, many start their scientific careers hoping to follow... documentation of my results, answer loads of questions, and I also had to do some troubleshooting as ...ahead and contact a technical support team with a question or two that open a dialogue. This will give you...there is considerable diversity in the types of questions customers ask, and there are also differences ... in a technical support role. What general characteristics do jobs in technical support have? There are...Correspondent or similar. While there isn’t a clear distinction between these job titles, they may be different...team accepts any incoming requests and answers questions that are less time consuming and/or less technically...

...Content last reviewed: 9 December 2025 Do you have suggestions for other plasmids that should be added to this...

...Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should be added to this...

...Marker PI Publication Back to Top Do you have suggestions for other plasmids that should be added to this...

...development of immunotherapeutics. There are still many important questions in this exciting field of research.... complicated and dynamic phenomenon that is interestingly responsible for tumor reduction as well as progression...inflammatory responses can augment tumor growth. Indeed, lasting responses to viral infections have resulted in ...chemical signals such as IL-8, IFNγ, and colony stimulating factor 1 (CSF-1). Additionally, tumor cells recruit...cell-surface proteome to inhibit expression of co-stimulatory molecules needed for T cell effector function...surfaces to avoid recognition by CD8+ T cells. Existing therapies and effects on immune system Conventional... However, these conventional therapies produce lasting side effects, damage healthy cells, and often cannot...

...expert technical support team ready to answer your questions. All Antibodies Ready to use antibodies? Browse...

...Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should be added to this...

...Content last reviewed: 9 December 2025 Do you have suggestions for other plasmids that should be added to this...

...Content last reviewed: 9 December 2025 Do you have suggestions for other plasmids that should be added to this...

...startled by acoustic, visual or tactile stimuli, larval zebrafish react by performing a distinct C-bend to... first 2 weeks of life, larvae already exhibit distinct behaviors such as spontaneous swimming and escape...neuron more positive or more negative.  Neuronal stimulation: Depolarization When the inside of the neuron... NpHR, can even be expressed simultaneously and still be individually activated with different lights ...behavior is not done in response to any specific stimulus. While the escape response neural pathway is well...groups were activated or “turned on.” In order to distinguish between swimming behaviors, the researchers measured...immobilized in agar at the head with their tails still free, and different regions of the spinal cord were...

...techniques and is a multi-step process consisting of digestion, purification, ligation, and transformation...of the polymerase generates sticky ends in the PCR reactions. These sticky ends are annealed and ligated...used techniques in the biological sciences and constitute foundational elements of biomedical research....transformation. While restriction cloning is still routinely performed by many labs, a variety of other cloning... of cloning has its advantages, most scientists still encounter several struggles with these techniques...Restriction Enzymes + - PCR Amplification + + Digestion + + Gel Purification + - Ligation + - Transformation...restriction enzyme DpnI to remove the templates. Digestion products are then transformed directly into chemically...

...Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should be added to this...