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We narrowed to 717 results for: CaS;

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Showing: 201 - 220 of 717 results

  1. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...sequences, which include the enzymes C2c2/Cas13a and Cas13b....VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In bacteria... This activity provides a stark contrast to Cas9 and Cas12, which require that each DNA target have high...to attentuate RNA levels. In mammalian systems, Cas13a does not exhibit the collateral RNA degradation...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you ...

  2. Tetracycline Inducible Expression

    Type
    Collection
    ...Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9-VPR transcriptional activator...tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP...Julien Béthune 154939 dCas13d-dsRBD-APEX2 RNA-protein interaction mapping via Cas13-based APEX targeting...your gene of interest should only be expressed occasionally, use Tet-On: express an rtTA or TetR and include...should be generally active and only turned off occasionally, use Tet-Off: express a tTA and include a tet-responsive...DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9-DHFR for CRISPRi. See Plasmid #167937 for CRISPRa...Dox-inducible expression of Cre recombinase. Madeline Lancaster 198752 DiLiCre 2.0 Dox-inducible expression of...

  3. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection...transcriptase (RT) is fused to the C terminus of Cas9 H840A nickase. The fusion enzyme is capable of installing...template. During prime editing, the pegRNA directs the Cas9 nickase to the target sequence where it nicks the...nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can be used in PE2...PE6 — small prime editors with optimized RT and/or Cas9 domains PE7 — addition of RNA-binding exonuclease...PuroTK-pegRNA_GG Mammalian, piggyBac hU6 pegRNA BsmBI Yes (Cas9 H840A + MMLV RT) PuroTK Jacob Giehm Mikkelsen 174038...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you ...

  4. AAV Molecular Tools

    Type
    Collection
    ...Engineering Tools These AAV encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome editing...AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA CMV-driven, constitutive Expression of human codon-optimized Cas9 from Staphylococcus...Expression System Activity Serotype PI 45580 pAAV-flex-taCasp3-TEVp EF1a-driven, Cre-dependent Cre-dependent, ..., bicistronic expression of designer pro-taCasp3 and TEVp for studying cell ablation. 1, 5, 8 Nirao Shah...Thomas Jessell , Eiman Azim 207997 pAAV hSyn-fDIO-taCasp3-TEVp Syn-driven, Cre-dependent Inducing apoptotic...Staphylococcus aureus (SaCas9). 6, 8 Feng Zhang Labeling Tools These AAV encode tools that can be used...

  5. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ... protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is going...going to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression of the RNA polymerase...for quick integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage 186. Detailed... Bikard, D., & Van Teeffelen, S. (2018). Tuning dCas9’s ability to block transcription enables robust,...Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in the chromosome... of the two reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐borne CRISPR ...

  6. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing the...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...E488Q) adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts...guanosine, the result is an A to G change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you ...

  7. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ... for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin...molecule-mediated ChIP) system, catalytically inactive dCas9 is used to purify genomic DNA bound by the gRNA....gRNA. An epitope tag or tags can be fused to dCas9 or gRNA for efficient purification. Various epitope tags...biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system . The...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you ...

  8. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... an exogenous donor cassette, the donor cassette replaces the target site cassette in a double-crossover... sites (often called a "lox-STOP-lox" or "LSL" cassette) placed upstream of a gene of interest will prevent...-layered genetic control. Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible...vivo using a mouse line expressing Cre-dependent Cas9. Kits Kit Name Type PI Description dRMCE Plasmid...Rolf Zeller Use this dual recombinase-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional...

  9. DNA Quantification

    Type
    Protocol
    ...light that your substance maximally absorbs. In the case of nucleic acid (DNA and RNA), the maximal absorbance...

  10. p53 Pathway

    Type
    Collection
    ...domain death agonist CASP3 Caspase 3, apoptosis-related cysteine peptidase CASP8 Caspase 8, apoptosis-related...apoptosis-related cysteine peptidase CASP9 Caspase 9, apoptosis-related cysteine peptidase Cyclin B CCNB1 CCNB2 CCNB3...

  11. Immunology Research Plasmids and Resources

    Type
    Collection
    ...mE10 CARD11 caspase recruitment domain family, member 11 BIMP3, CARMA1, MGC133069 CBL Cas-Br-M (murine...retroviral transforming sequence C-CBL, CBL2, RNF55 CBLB Cas-Br-M (murine) ecotropic retroviral transforming sequence...DKFZp779F1443, FLJ36865, FLJ41152, Nbla00127, RNF56 CBLC Cas-Br-M (murine) ecotropic retroviral transforming sequence...BRAF1, FLJ95109, MGC126806, MGC138284, RAFB1 CASP3 caspase 3, apoptosis-related cysteine peptidase CPP32...ASBT, ISBT, NTCP2 TAP1 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) ABC17, ABCB2, APT1, D6S114E...TAP1*0102N, TAP1N TAP2 transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) ABC18, ABCB3, APT2, D6S217E...IMD1, MGC126261, MGC126262, PSCTK1, XLA CARD11 caspase recruitment domain family, member 11 BIMP3, CARMA1...

  12. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions...create stable cell lines in which the expression cassette of interest is incorporated into the host...Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression pnCasPA-BEC - A cytidine deaminase-mediated base-editing... Separate expression pRGEB31 - Deliver sgRNA and Cas9 into plants by Agrobacterium-mediated transformation...Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated...

  13. Video Library

    Type
    Protocol
    ... deletions in mammalian cells lines using CRISPR/Cas9 Genomic Deletions with CRISPR Protocol Over-Agar...

  14. Centrifugation

    Type
    Protocol
    ... not dislodge any pellets or re-mix liquids. In cases where you are separating solids from a liquid, pellets...

  15. Neurodegeneration Research Collection

    Type
    Collection
    ...familial ALS cases having at least one other affected family member. These familial cases, usually inherited...range of cellular processes. The vast majority of cases (90–95%) of ALS are sporadic, having no prior family...cause) nature of the disease, a small portion of cases can be attributed to known genetic factors such ...early-onset form accounts for less than 10 percent of all cases of Alzheimer's disease. Of these early-onset forms...

  16. Rett Syndrome

    Type
    Collection
    ...typically arise spontaneously, although in rare cases Rett syndrome can be inherited. In females, random...R306C) account for almost 46% of all Rett syndrome cases. Research Milestones 1992 - MECP2 is discovered ...Knock-in of NLuc-tdTomato at endogenous MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional...hand use in girls: Rett’s syndrome: report of 35 cases. Ann Neurol . 14, 471–479. (Link opens in a new ...

  17. Fluorescence Titering Assay

    Type
    Protocol
    ...transduced with a range of dilutions of pHAGE-TO-dCas9-3XmCherry . 72 h post transduction, cells were assayed...

  18. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...Plant Yeast Resources Catalytically dead Cas9 (dCas9), or dCas9 fused to a transcriptional repressor peptide...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you ...

  19. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...Plant C. elegans Yeast Resources Catalytically dead dCas9 fused to a transcriptional activator peptide can...specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your... separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Figure 1: Overview...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you ...

  20. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...markers. The FP together with the protothropic cassette can be amplified with the following primers: FW...Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab Materials... Sheff, M. A., Thorn, K. S. (2004). Optimized cassettes for fluorescent protein tagging in Saccharomyces...., Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection markers for ...
Showing: 201 - 220 of 717 results