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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 413 results for: his

Showing: 221 - 240 of 413 results

  3. TALEN Plasmids and Kits

    Type
    Collection
    ... was recently shown that TALE fusions with the histone demethylase LSD1 (Lysine specific demethylase) ...with the FLASH method can decrease the amount of Histone H3 lysine 4 (H3K4) di-methylation at the target...

  4. Sequencing Primers

    Type
    Guide
    ...reverse primer pTrcHis Forward GAGGTATATATTAATGTATCG (Invitrogen) 5' of MCS in pTrcHis vector, forward...forward primer pTrcHis Reverse GATTTAATCTGTATCAGG (Invitrogen) 3' of MCS in pTrcHis vector, same as pBAD-R,...primer HAT GAGGAGCACGCTCATGCCCAC (BD Biosciences) Histidine affinity tag, forward primer hGH-PA-R CCAGCTTGGTTCCCAATAGA...

  5. Promoters

    Type
    Guide
    ...general transcription factor proteins and histones can bind. Histones are proteins found in eukaryotic cells...cells that package DNA into nucleosomes. Histone binding prevents the initiation of transcription whereas...

  7. All Antibodies

    Type
    Collection
    ...species. We currently assess western blot, immunohistochemistry, and immunocytochemistry. Addgene will continue...

  8. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Lee et al., 2013), and are available with URA3, SpHIS5 and KANMX markers. The FP together with the protothropic...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...large-scale expression screen that can be used to generate His-tagged human kinase constructs that express well ... Saccharomyces strains by compensating for histidine (HIS3), leucine (LEU2), uracil (URA3), methionine...epigenetic modifiers like DNA methyltransferases or histone deacetylases. Unfortunately, these inhibitors are...were validated by both Western blotting and immunohistochemistry, and are summarized in the table below: ...

  10. CRISPR Plasmids and Resources

    Type
    Collection
    ...discover links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial...

  11. Antibody Production

    Type
    Collection
    ...is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections are fixed, permeabilized...

  13. AAVED

    Type
    Collection
    ...the lab (e.g., controls to perform, recommended histology conditions, minimum/maximum incubation time after...

  14. Microbiology Resources

    Type
    Collection
    ...Prions Plasmids for Protozoa Species Entamoeba histolytica Leishmania sp. Plasmodium sp. Toxoplasma gondii...

  15. p53 Pathway

    Type
    Collection
    ...TP53 dependent G2 arrest mediator candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2 SESN3 Sestrins...

  17. Lentivirus Plasmids

    Type
    Collection
    ...plasmid 14749 for Thy1.1 selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of...

  18. CRISPR Guide

    Type
    Collection
    ... cytosines in a gene’s promoter or by inducing histone acetylation or demethylation. The most common modifiers...153 (4), 910–918. PMID: 23643243 Zhang, Y., & Marchisio, M. A. (2020). Type II anti-CRISPR proteins as...

  20. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGGCCAAAGAAAAGGACGcgataagaaagtttgtTGTGGCTCAACTGCAGAA hist2h3ca1 TAL3284 & TAL3285 TCTGTTTGAGGACACCAAcctgtgcgccatccacgCCAAGAGGGTCACCATCA...
Showing: 221 - 240 of 413 results