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We narrowed to 444 results for: CRISPR

Showing: 241 - 260 of 444 results

  1. CRISPR Plasmids - Double-Strand Break (Cut)

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully... CRISPR Cut CRISPR Plasmids: Cut Browse CRISPR...CRISPR plasmids that create double strand breaks in DNA. CRISPR... Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR plasmids designed to introduce a DSB. Plasmids ...

  2. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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    Blog Post
    ...Resources at Addgene.org Check out our CRISPR resources and CRISPR for plants Bacterial Expression Systems...PZA-resistance by using different approaches, including CRISPR and recombination-mediated genetic engineering ...more information on bacterial engineering using CRISPR on our blog). Her research should provide us with...

  3. Addgene's Top Blog Posts from 2020

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    Blog Post
    ... Based on CRISPR/Cas by Shravanti Suresh Guest blogger Shravanti Suresh who studies CRISPR at Iowa State...State University detailed five methods for using CRISPR to detect SARS-CoV-2, including many with plasmids...

  4. Microbiology Resources

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    ...Microbiology General CRISPR Resources Read our CRISPR Guide for a general introduction to CRISPR technology. Check...software. Addgene yeast CRISPR resources Bacterial CRISPR Resources The CRISPR technology was developed...synthetic biology. CRISPR Resources : Find essential background information on using CRISPR in your lab, specific... Check out our CRISPR reference page for CRISPR resources, such as depositor protocols and gRNA design...Find CRISPR tools for use in genome editing of bacteria. CRISPathBrick Multiplex Plasmid : CRISPR expression... specific CRISPR tools for genome editing in bacteria, and more. Synthetic Biology : Browse our deposited...editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome Engineering blog post...

  5. CRISPR Plasmids - Activate Gene Expression

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...Activate CRISPR Plasmids: Activate Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR... CRISPR Activate...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR plasmids for transcriptional activation. Plasmids...

  6. CRISPR Plasmids - Xenopus

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Xenopus CRISPR Plasmids: Xenopus Browse...CRISPR plasmids that have been designed for use in Xenopus. CRISPR... Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation... The following CRISPR plasmids have been designed for use in Xenopus. Cut Fully functional Cas9 enzymes...selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and...

  7. Design Tips for Prime Editing

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    Blog Post
    ...Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by ...editing guide RNA (pegRNA). The pegRNA consists of a CRISPR gRNA extended to include a primer binding site ...Prime Editing: Adding Precision and Flexibility to CRISPR Editing PRIDICT: Predicting Efficiencies of Prime...

  8. Lentiviral Vector Uses and Overview

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    Blog Post
    ...Lentiviral vectors are the vector of choice for many CRISPR applications, and they’ve also had success in clinical...transgene-expressing or knockout cell lines. Many CRISPR plasmids are designed for lentiviral use, including... Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods...Lentiviral Plasmid FAQs Answered Learn How Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out...including Addgene’s top requested plasmid in 2015: lentiCRISPR v2, used both for targeted modification and genome-wide...

  9. CRISPR Plasmids - Base Edit

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break. CRISPR... CRISPR Base Edit CRISPR...CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the Bassik lab , in which Cas9 is fused ...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR base editing plasmids. Plasmids are available for...

  10. CRISPR Plasmids - C. elegans

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR C. elegans CRISPR Plasmids: C. ...following CRISPR plasmids have been designed for use in C. elegans. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in C. elegans. CRISPR.... elegans Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and...

  12. CRISPR Plasmids - Drosophila

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR... CRISPR Drosophila CRISPR...CRISPR Plasmids: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime... The following CRISPR plasmids have been designed for use in Drosophila and other insects. Cut Fully functional... functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based...Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...

  13. Zebrafish Plasmid Collection

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    ...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology...examples of useful zebrafish CRISPR resources can be found below: UFlip CRISPR targeted integration vectors...for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression...Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...for the laboratory use of zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been...morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart...

  14. CRISPR Plasmids - Mammalian Expression

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR... CRISPR Mammalian Expression CRISPR...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...mammalian expression systems. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (...Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...->G (or T->C on the opposite strand) mutations. CRISPR-X, a base-editing system from the Bassik lab, uses...

  15. Genomic Deletions in Mammalian Cell Lines

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    ... CRISPR References & Information Genomic Deletions in Mammalian Cell Lines CRISPR: Protocol...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud...regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system may be re-purposed.... Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting CRISPRs into Cells of Interest NOTE: ...using CRISPR/Cas9 CRISPR...Orkin's and Daniel Bauer's labs details the use of CRISPR/Cas9 to create genomic deletions in mammalian cell...of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9. Bauer DE, Canver MC, Orkin SH. J. Vis. Exp...

  16. CRISPR Plasmids - Bacteria

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base... CRISPR Bacteria CRISPR Plasmids: Bacteria...following CRISPR plasmids have been designed for use in bacteria. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in bacteria. CRISPR...Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...Insert Promoter PI Publication RNA Targeting Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and ...

  17. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post
    ...Chromatin Interactions: Using CRISPR-dCas9 to Study Gene Regulation CRISPR 101: Epigenetics and Editing...Studying Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR Between the Genes: How to Experiment...or DNA repair, or replication, or any number of CRISPR applications and thought “OK, but how does this...

  18. CRISPR Plasmids - Yeast

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Yeast CRISPR Plasmids: Yeast Browse ... following CRISPR plasmids have been designed for use in yeast. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in yeast. CRISPR...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ... Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and...

  19. PiggyBac-ing Through the Genome Editing Field

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    Blog Post
    ...piggyBac™ Transposon Catch up on Everything CRISPR with Our CRISPR Guide Deposit Your piggyBac™ Vectors! ... to the development of site-specific nucleases (CRISPR/Cas9, TALENs, and ZFNs). However, several challenges...piggyBac™ transposon system can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations...interest, along with a site-specific nuclease (such as CRISPR/Cas9) to create a double-stranded DNA break. After...technologies including Zinc Fingers, TALENs, and CRISPR-Cas9 nucleases. Mathew applies his specialized ...

  20. Zhang Lab's CRISPR Frequently Asked Questions

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    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR FAQs Zhang...Zhang Lab's CRISPR Frequently Asked Questions You may also like... Zhang Lab CRISPR plasmids General CRISPR...using the lab's CRISPR technology. Visit the Zhang Lab page and forum to learn more. CRISPR Design FAQs Homologous...the Bench FAQs CRISPR Design FAQs Should I use wildtype or double nickase for my CRISPR genome engineering...Dr. Le Cong, the first author of a seminal CRISPR article from Dr. Feng Zhang's Lab, has assembled a ... lab's CRISPR technology. Genome... CRISPR Plasmids Dr. Le Cong, the first author of one of the seminal articles from Dr. Feng Zhang's Lab...
Showing: 241 - 260 of 444 results