We narrowed to 931 results for: TIM

...rEcoli XpS (Bacterial strain #192872) , and an optimized phosphoserine orthogonal translation system, pSerOTS-C1... C321.ΔA (Bacterial strain #68306) , with the optimized phosphoserine orthogonal translation system, SepOTSλ...Rogulina, S., & Rinehart, J. (2023). System‐wide optimization of an orthogonal translation system with enhanced...

... for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive... Fibroblasts Cardiomyocytes Lentiviral Mouse Optimization of direct fibroblast reprogramming to cardiomyocytes...transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons. Elife...

...infectious particle production, including codon optimization, deletion of the C-terminal 18-21 amino acids...SARS-CoV materials are available only in the US at this time. Accessory Plasmids Envelope and Packaging Plasmids...

...but are difficult to generate due to expense and time. An alternative approach is to use synthetically...include consistency between lots and the ability to optimize the antibody’s antigen binding sequence to improve...

...constructs in precise cell types and/or developmental timeframes. Here we highlight some of the plasmids and tools...including lacZ and GFP fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available with...

...Ras can activate a variety of pathways, which ultimately turn on genes involved in cell growth, differentiation... 1 RALGDS Ral guanine nucleotide dissociation stimulator RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide...

...targeting : Cas13 enzymes target RNA rather than DNA, sometimes requiring a protospacer flanking sequence (PFS...imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell . 155(7):1479-91. PMID...

...a protein so that it is responsive to specific stimuli, such as light. On a broader scale, the expanded... to UAG, RF1 function removed, improved doubling time. George Church 98564 C321.Ub-UAG-sfGFP all TAG sites...

...growth rate, so my cell pool is going to evolve over time, which is bad news for repeatability and bad news...had never done it before and it worked the first time probably because I used a robust cell line. If your...The Surveyor kit had a lot of good tips for first time users, all of which I followed. If you have never...the duplex and run it as an undigested control. Sometimes the PCR products can look smeared, so having an...products (GFP-GFP duplex) as a control. 4. After optimizing the reaction and confirming the presence of the...landscape of research opportunities, it’s an exciting time to be in the lab. Based on the power of available...John Doench & David Root and are described in Optimized sgRNA design to maximize activity and minimize...

...gene of interest. These modifications persist over time and are potentially heritable in dividing cells....

...tag, TRX, or V5) Mutations Wild type (wt) Codon-optimized expression (recode) Truncated for making pseudovirus...

...target-specific gRNA in the same cell at the same time. All-in-one plasmids ensure that both the Cas and...

...Plasmids This plasmid collection contains codon-optimized ORF sequences for human SLCs in Gateway-compatible...

... Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with pSicoligomaker (Andrea...

...Cas9’s. Further advancements have been made by optimizing expression of the fusions, modifying the linker...

...mutant clones from watermelon have been codon-optimized for expression in bacteria and cloned into an ...

...Taek Soon Lee Joshua Leonard Han Lim Wendell Lim Timothy Lu Michel Maharbiz Thorsten Mascher Kathleen Matthews...

...technology (Link opens in a new window) and an optimized sorting process. This combination allows IPI to...

...generated using standard methods individually optimized for each specific antibody in order to generate...

... production plasmids that have been carefully optimized for both safety and efficiency. Learn more about...

... variety of cell types. Protein Purification Optimization Kit Using SUMO Protease Elution Protein Characterization...

...acids, their repeat-variable di-residue (RVD). Ultimately, it is this RVD that determines which single ...

...Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance...

...CMV-driven, constitutive Expression of human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). 6, 8...

... and viral safety. AAV Vector Guide Want to save time? Our Viral Vector Packaging Service has hundreds...

...Rodriguez-Estevez, et al. 2020. Transduction optimization of AAV vectors for human gene therapy of glaucoma...

... EGFP. Can be used for cDNA expression. David Baltimore 12247 pLVTHM 2nd EF-1a-driven GFP and shRNA under...

...Did you catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If...webinar! Special thanks to our panel of experts, Tim Miles, David Goertson, Xinhong Chen, and Miggy Chuapoco...didn’t get answered live - but he kindly took some time to address all your unanswered questions via text...Chuapoco, and to all our participants for their time and excellent questions.    Additional Resources...Adenoviral delivery of CRISPR/Cas9 Tips for a first time AAV user Resources on Addgene.org Find Adenovirus...

..._3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044) Optimized expression of SYFP2 (yellow fluorescent protein...

...Gene Type Wildtype Mutant Partial Sequence Codon-Optimized gRNA Other Tags GFP FP other Flag GST His TEV ...Gearhart 44333 pMX_mTBP TBP LTR Parkinson's Marc Timmers 44978 GST-FUS-WT FUS GST tac ALS Robin Reed 44981... CMV DJ-1 C106A PARK7 mTagBFP2 CMV Parkinson's Timothy Ryan 221331 pFastBac-GST-TBK1 TBK1 His, TEV, GST....1 mTagBFP2 rat DJ-1 shRNA PARK7 U6 Parkinson's Timothy Ryan 223563 pmCherry-Synaptojanin-1-145 SYNJ1 His...