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...often closely related to its function. There are a variety of methods for determining subcellular localization...fluorescent fusion proteins that are targeted to various organelles or subcellular structures. To compare... General Structures Eukaryotic cells contain a variety of organelles and subcellular structures, each ...

...including calcium sensors, under the control of various promoters. Sensor Green Calcium Sensors GCaMP8 ...Calcium Sensor: GCaMP7c 105321 pGP-AAV-syn-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Constitutive 1 Douglas...Douglas Kim 105322 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Cre 1 Douglas Kim Calcium...

...to generate and characterize single nucleotide variant lines in an isogenic cellular background. All cell...create cell lines with endogenously-tagged gene variants or to differentiate human iPSCs into other cell...

...for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s ...for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2-dTom-nlsdTom...

... community demonstrating AAV performance under various experimental conditions. Protocols Written and ...and scientists across the research community on various aspects of viral vectors, including troubleshooting...

... with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. ...

...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive overexpression...

...Addgene's CRISPR collections feature plasmids for a variety of organisms, including mammals, plants, bacteria...an empty vector for your experiment based on a variety of factors. Browse Plasmids by Model Organism Bacteria...

...applications. The specific QC experiments performed varies for each antibody catalog item. Whenever possible...primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified, sequenced...

...stably integrate transgenes of interest into a variety of cell types. Wild-type gamma-retroviruses have...the Weinberg Lab page for additional resistance variants. Bob Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression...

...mTORC2 The better-characterized mTORC1 responds to various signals, including amino acids, stress, oxygen,...prognosis in many cancers, including breast, colon, ovarian, and liver cancers. mTORC1 is activated downstream...

...which have been developed for special functions that vary from purification, visualization, and functional...specific purpose. HaloTag technology is optimized for various protein expression systems including E. coli , ...

...: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget...Germline TP53 mutations and Li-Fraumeni syndrome. Varley JM. Hum Mutat. 2003 Mar;21(3):313-20. PubMed PMID...

...express your gene of interest under the control of a variety of promoters. Professor Oka and the Gene Vector...can be used to generate adenovirus expressing a variety of reporters as well as the Cre and Flp recombinases...

...standards and typical titers, though actual yields may vary by construct. Size Quantity Total Volume Minimum...processing your order. MTA Approval 1+ business days, varies by requesting organization Viral vector preps remain...

...AAV6 Vectors: Strategies for Overcoming Donor-Variation and Implications in Genome Editing. Sci Rep . ...

...Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Return to top Other Coronavirus Plasmids: SARS-CoV...Screening Jesse Bloom Tyler Starr Libraries of variant Spike protein RBD mutations for comprehensive mapping...

...Site Canonical Sequence Structure (Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT 13...results. Attenuated iCre(R297T), a point mutant iCre variant, reduces the number of recombination events but...

...create cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection of Stem ...c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming to pluripotency...

...effective brightness of a fluorescent protein may vary depending on experimental conditions. pKa is the... to top Fluorescent Timers Fluorescent protein variants that change their fluorescence from the blue to...

...have deposited plasmids include: Joan Brugge , Harvard Medical School Klaus Hahn , UNC Richard Hynes ,...

...created a number of plasmids for expression of variants of LRRK2 , SNCA , and other proteins of interest...

...Joung and Daniel Voytas have deposited at Addgene various reagents for engineering and expressing zinc finger...

...Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University Epigenetic Regulators of Intestinal ...

...comprehensive collection of ORFs tagged with a variety of fluorescent proteins. The majority of the ORFs...

... scientists. Additionally, we have developed a variety of educational resources to help you choose and...

... activating or upregulating gene expression in various model organisms....

...fused to dCas9 or gRNA for efficient purification. Various epitope tags including 3xFLAG-tag, PA, and biotin...

...The plasmids available in this format include a variety of popular plasmids and backbones with high cloning...

...photoreceptors. Spectrally distinct fluorescent iRFP variants have been shown to have high brightness and allow...