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We narrowed to 795 results for: Tec;

Showing: 41 - 80 of 795 results

  1. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ... heterodimeric FokI domain. Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be ...heterodimeric FokI domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802...heterodimeric FokI domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMG10 ...

  2. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ... of detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like...imaging has numerous advantages over other imaging techniques, including that it is easy to implement due to...FISH), CRISPR imaging offers a unique method for detecting the chromatin dynamics in living cells. The fluorescent...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology 2013). We also have an older system using two...express up to three sgRNAs (Swiech et al., Nature Biotechnology 2014). Full references are below. Available ...mouse CRISPR-Cas9 is a versatile genome editing technology for studying the function of genetic elements...Cradick TJ, Marraffini LA, Bao G, Zhang F. Nat Biotechnol. 2013 Sep;31(9):827-32. doi: 10.1038/nbt.2647...Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6. doi: 10.1038/nbt.3055...

  4. Genetic Code Expansion

    Type
    Collection
    ...104069 pTECH-chAcK3RS(IPYE) AcK3RS Chimeric Nε-acetyl-L-lysine Bacterial TAG David Liu 104070 pTECH-MbAcK3RS...104071 pTECH-MmAcK3RS(IPYE) AcK3RS M. mazei Nε-acetyl-L-lysine Bacterial TAG David Liu 104072 pTECH-MbPylRS...M. mazei Cyanobacterial TAG Lital Alfonta 99222 pTECH-chPylRS(IPYE) PylRS Chimeric p-iodo-L-phenylalanine...-L-phenylalanine Bacterial TAG David Liu 104073 pTECH-MmPylRS(IPYE) PylRS M. mazei m-iodo-L-phenylalanine...

  5. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...popular SepOTSλ (Plasmid #68292) . An important technical note: pSerOTS-C1* (V70) is a ROP minus plasmid...split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent protein-peptide interactions...kinases of interest by mass spectrometry in a technique called serine-oriented library/kinase-library ...phosphorylation-dependent interactions . Nature Biotechnology , 36 (7), 638–644. https://doi.org/10.1038/nbt...

  6. Luciferase Plasmid Collection

    Type
    Collection
    ...Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent... luciferases require different substrates and detection wavelengths. Firefly luciferase is brighter than...investigating regions controlling transcription Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating...investigating regions controlling transcription Pete Stecha 106292 pLS-SV40-mP-Rluc Renilla Insertion of 5'...

  7. Tetracycline Inducible Expression

    Type
    Collection
    ...binding of Tet-Advanced transactivator (developed by Clontech, based on Resnitzky et al., 1994). TRE3G promoter...alternative effectors for Tet transregulators . BioTechniques, 37 (4), 546–550. https://doi.org/10.2144/04374BM04...promoters with minimized background expression . BMC Biotechnol, 10 , 81. https://doi.org/10.1186/1472-6750-10...opens in a new window) Tet Systems: Science and Technology (Link opens in a new window) Do you have suggestions...

  8. AAV Viral Preps

    Type
    Collection
    ...can be safely handled at the lowest biosafety protection levels. High quality and high titer in-stock ...retrograde Retrograde AAV To target projection neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1, MaCPNS1...AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, AAVrg, the Caltech systemic serotypes, or others, it will be listed...

  9. Institute for Protein Innovation

    Type
    Collection
    ...opens in a new window) based on yeast display technology (Link opens in a new window) and an optimized...collection includes anti-glypicans that accurately detect glypican presence and explore glypican function...that recognize common epitope tags to help purify, detect, or localize particular proteins. Browse or search...

  10. Plan Your Experiment

    Type
    Collection
    ...editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006 PMID:...more information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different... analysis of CRISPR–Cas experiments. Nature Biotechnology , 38 (7), 813–823. https://doi.org/10.1038/s41587...

  11. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England...fixed cells. The plasmids associated with this technology are available at Addgene for distribution to ...NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag SNAP-tag is based on the human O6-alkylguanine-DNA-alkyltransferase...

  12. Antibody Plasmid Collection

    Type
    Collection
    ...proteins, visualize a protein under a microscope, or detect when the protein is present in a sample — just ...Vector system for expression, dimerisation and detection of recombinant antibody fragments in the form ...and utilisation of recombinant antibodies. BMC Biotechnol. 2006 Dec 7;6:46. John McCafferty CRISPR system...

  13. Distribution to Industry

    Type
    Collection
    ... Proteins Mikel Garcia-Marcos 10 plasmids for detecting GPCR activity in a variety of cell types. Protein...intellectual property rights, and provides liability protection for material providers. Addgene's deposit agreements...distribution. Please see the Sample Agreements on our Technology Transfer Information page for more information...

  14. Plant Plasmids and Resources

    Type
    Collection
    ...spanning a wide variety of molecular biology technologies: from genome engineering and gene regulation...CRISPR Guide for a general introduction to CRISPR technology or the Plant CRISPR Resources for a full selection...initiative promotes interdisciplinary exchange, open technologies, and responsible innovation for improvement ...

  15. Zebrafish Plasmid Collection

    Type
    Collection
    ...CRISPR Guide for a general introduction to CRISPR technology or the zebrafish CRISPR resources for a full ...zebrafish resources and developing new lines and technology. European Zebrafish Resource Center (EZRC) - ...- A repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish lines ...

  16. TALEN Plasmids and Kits

    Type
    Collection
    ...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction...48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b vectors were designed...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...

  17. Rett Syndrome

    Type
    Collection
    ... Stem Cell Research page. CRISPR Tools CRISPR technology is a promising therapeutic approach for precise...CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR resources for a full ...Anti-MECP2 (D4F3) rabbit antibody from Cell Signalling Technology Additional Resources (Link opens in a new window...

  18. Zinc Finger Consortium Reagents

    Type
    Collection
    ...research and development of engineered zinc finger technology. Consortium members Keith Joung and Daniel Voytas... nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents CRISPR Plasmids...

  19. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...given region of the genome. Like nickase technology, this technique reduces unwanted off-target effects of...

  20. Antibodies

    Type
    Collection
    ...you choose and use antibodies, with an expert technical support team ready to answer your questions. All... antibody production and storage techniques, and explain common applications. View Guide ...

  21. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...Additional Resources Addgene's Data Hub Curated technical reports from the research community demonstrating...troubleshooting advice and explanations of viral vector technologies. Penn Vector Core AAV Vectors Many AAV vectors...

  22. Resolute Plasmid Collection

    Type
    Collection
    ...inclusive, ‘open-access ethos’ to the results, techniques, and reagents with the highest-possible quality... research as well as applied research in small biotech and pharmaceutical companies. The RESOLUTE project...

  23. E11 Bio PRISM Collection

    Type
    Collection
    ..."), creating over 100,000 unique combinations detectable by antibodies. All of the PRISM protein tags ...individual or subsets of barcode proteins to label and detect multiple injection sites in the same tissue, as...

  24. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...exonuclease protection factor La to PEmax to enhance pegRNA stability epegRNA — additional protection added ...

  25. Microbiology Resources

    Type
    Collection
    ...CRISPR Guide for a general introduction to CRISPR technology. Check out our CRISPR reference page for CRISPR...resources Bacterial CRISPR Resources The CRISPR technology was developed from bacterial immune systems, ...

  26. Worm Expression Resources

    Type
    Collection
    ...CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR resources include plasmids... website . Co-CRISPR plasmids that facilitate detection of genome-editing events. Developed in Craig Mello's...

  27. AAV Packaged on Request

    Type
    Collection
    ...can be safely handled at the lowest biosafety protection levels. We offer 1,051 in-stock viral vector ...well-developed and trusted protocols and peer-reviewed techniques (Link opens in a new window) for producing high-quality...

  28. Neurodegeneration Research Collection

    Type
    Collection
    ...neurons function. They are also delving into new technologies and resources in order to gain more fundamental...mitochondrial DNA using mitoBEs . Yi et al. Nat Biotechnol. 2023 May 22. See More AAV Viral Preps Find AAV...

  29. CRISPR Plasmids - Tagging

    Type
    Collection
    ...traditional tagging methods. Several different tagging techniques, as well as the plasmids and protocols needed...Förstemann lab has developed a CRISPR tagging technique for use in Drosophila cells that uses PCR to generate...

  30. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...dividing cells. CRISPR activation and interference technologies also indirectly modify local chromatin state...

  33. CRISPR Plasmids and Resources

    Type
    Collection
    ... CRISPR Plasmids and Resources CRISPR technologies are continously developing and expanding. Addgene...

  35. Synthetic Biology - Overview

    Type
    Collection
    ...papers on Syn Bio SEVA - Standard European Vector Architecture Open Plant - Plant SynBio resource Synthetic...

  37. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ... within this set are derived from pAdenoX from Clontech and can be used to generate adenovirus expressing...

  39. Open Enzyme Collection

    Type
    Collection
    ...immunology, biochemistry, and more. The foundational technologies enabled by this collection include polymerase...

  40. Ras Pathway

    Type
    Collection
    ...use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of RAS pathway gene...
Showing: 41 - 80 of 795 results