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Showing: 41 - 80 of 794 results

  1. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ... heterodimeric FokI domain. Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be ...heterodimeric FokI domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802...heterodimeric FokI domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMG10 ...

  2. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ... of detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like...imaging has numerous advantages over other imaging techniques, including that it is easy to implement due to...FISH), CRISPR imaging offers a unique method for detecting the chromatin dynamics in living cells. The fluorescent...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology 2013). We also have an older system using two...express up to three sgRNAs (Swiech et al., Nature Biotechnology 2014). Full references are below. Available ...mouse CRISPR-Cas9 is a versatile genome editing technology for studying the function of genetic elements...Cradick TJ, Marraffini LA, Bao G, Zhang F. Nat Biotechnol. 2013 Sep;31(9):827-32. doi: 10.1038/nbt.2647...Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6. doi: 10.1038/nbt.3055...

  4. Bacterial Expression Systems

    Type
    Collection
    ...bacterial genomes for your research. Plasmid ID Technique PI Purpose pCas9 42876 CRISPR Luciano Marraffini... expressed in vivo . Plasmid ID Visualization Technique/Tag PI Purpose Empty Backbones with Fluorescent...Reporter Plasmids Reporter plasmids can be used to detect events and molecules in and around a bacterium....small molecule, the reporter plasmid can be used to detect the presence of that small molecule and sometimes...) in the plasmid. Other similar reporters for detecting maltose can be found here . pAra Series Various...

  5. Zhang Lab's CRISPR Frequently Asked Questions

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    Collection
    ...assembled a list of FAQs about using the lab's CRISPR technology....assembled a list of FAQs about using the lab's CRISPR technology. Visit the Zhang Lab page and forum to learn ...addition to mutations in the spacer itself, will protect the HR template from the Cas9. Again, if it's within...of the Cong, et. al, 2013 Science paper). If you detect positive HR, isolate single-cell colonies, grow...

  6. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...of genomic deletions. In addition, we describe techniques for deletion, identification, and characterization...bp from the predicted cleavage site to ensure detection would not be impacted by a small indel at the ...in Molecular Biology. 2001. PubMed . MicroRNA detection in prostate tumors by quantitative real-time PCR...PubMed . Western blotting: sample preparation to detection. Eslami A, Lujan J. J. Vis. Exp . 2010...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent...more about pHlash sensor in our 28 Hot Plasmid Technologies from 2015 blog post. RNA Biology CMV-LUC2CP/...investigating regions controlling transcription Pete Stecha 212933 pGL4.84(hRlucCP/Puro) Renilla Vector for...investigating regions controlling transcription Pete Stecha Return to Top Expression Constructs Luciferase ...

  8. AAV Viral Preps

    Type
    Collection
    ...can be safely handled at the lowest biosafety protection levels. High quality and high titer in-stock ...retrograde Retrograde AAV To target projection neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1, MaCPNS1...serotypes AAV1, AAV2, AAV5, AAV8, AAV9, AAVrg, the Caltech systemic serotypes, or others, it will be listed...

  9. Institute for Protein Innovation

    Type
    Collection
    ...opens in a new window) based on yeast display technology (Link opens in a new window) and an optimized...collection includes anti-glypicans that accurately detect glypican presence and explore glypican function...that recognize common epitope tags to help purify, detect, or localize particular proteins. Browse or search...

  10. New England Biolabs Cell-Imaging Plasmid Collection

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    Collection
    ...England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England...fixed cells. The plasmids associated with this technology are available at Addgene for distribution to ...NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag SNAP-tag is based on the human O6-alkylguanine-DNA-alkyltransferase...

  11. Antibody Plasmid Collection

    Type
    Collection
    ...proteins, visualize a protein under a microscope, or detect when the protein is present in a sample--just to...Vector system for expression, dimerisation and detection of recombinant antibody fragments in the form ...and utilisation of recombinant antibodies. BMC Biotechnol. 2006 Dec 7;6:46. John McCafferty CRISPR system...

  12. Plant Plasmids and Resources

    Type
    Collection
    ...spanning a wide variety of molecular biology technologies: from genome engineering and gene regulation...CRISPR Guide for a general introduction to CRISPR technology or the Plant CRISPR Resources for a full selection...initiative promotes interdisciplinary exchange, open technologies, and responsible innovation for improvement ...

  13. Zebrafish Plasmid Collection

    Type
    Collection
    ...CRISPR Guide for a general introduction to CRISPR technology or the zebrafish CRISPR resources for a full ...zebrafish resources and developing new lines and technology. European Zebrafish Resource Center (EZRC) - ...- A repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish lines ...

  14. TALEN Plasmids and Kits

    Type
    Collection
    ...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction...48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b vectors were designed...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...

  15. Tetracycline Inducible Expression

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    Collection
    ...Krueger C, Pfleiderer K, Hillen W, Berens C. Biotechniques . 2004 Oct;37(4):546, 548, 550. PubMed . Optimization... R, Heinz N, Hampf M, Bujard H, Gossen M. BMC Biotechnol . 2010 Nov 24;10:81. doi: 10.1186/1472-6750-10...-10-81. PubMed . Tet Systems: Science and Technology (Link opens in a new window) Do you have suggestions...

  16. Rett Syndrome

    Type
    Collection
    ... Stem Cell Research page. CRISPR Tools CRISPR technology is a promising therapeutic approach for precise...CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR resources for a full ...Anti-MECP2 (D4F3) rabbit antibody from Cell Signalling Technology Additional Resources (Link opens in a new window...

  17. Zinc Finger Consortium Reagents

    Type
    Collection
    ...research and development of engineered zinc finger technology. Consortium members Keith Joung and Daniel Voytas... nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents CRISPR Plasmids...

  18. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...given region of the genome. Like nickase technology, this technique reduces unwanted off-target effects of...

  19. Antibodies

    Type
    Collection
    ...you choose and use antibodies, with an expert technical support team ready to answer your questions. All... antibody production and storage techniques, and explain common applications. View Guide ...

  20. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ... genes in zebrafish (Hwang and Fu et al., Nat Biotechnol. 2013). The Joung lab has also modified their...use in mammalian cells SpCas9 variants with no detectable off-target effects Modified PAM specifity of ...

  21. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...Viral Vector Resources Addgene's Data Hub Curated technical reports from the research community demonstrating...troubleshooting advice and explanations of viral vector technologies. Penn Vector Core AAV Vectors Many AAV vectors...

  22. Resolute Plasmid Collection

    Type
    Collection
    ...inclusive, ‘open-access ethos’ to the results, techniques and reagents with the highest-possible quality... research as well as applied research in small biotech and pharmaceutical companies. The RESOLUTE project...

  23. Distribution to Industry

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    Collection
    ...intellectual property rights, and provides liability protection for material providers. Addgene’s deposit agreements...distribution. Please see the Sample Agreements on our Technology Transfer Information page for more information...

  24. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...exonuclease protection factor La to PEmax to enhance pegRNA stability epegRNA - additional protection added ...

  25. Microbiology Resources

    Type
    Collection
    ...CRISPR Guide for a general introduction to CRISPR technology. Check out our CRISPR reference page for CRISPR...resources Bacterial CRISPR Resources The CRISPR technology was developed from bacterial immune systems, ...

  26. Worm Expression Resources

    Type
    Collection
    ...CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR resources include plasmids... website . Co-CRISPR plasmids that facilitate detection of genome-editing events. Developed in Craig Mello's...

  27. AAV Packaged on Request

    Type
    Collection
    ...can be safely handled at the lowest biosafety protection levels. We offer 963 in-stock viral vector preps...well-developed and trusted protocols and peer-reviewed techniques (Link opens in a new window) for producing high-quality...

  28. Lentivirus Plasmids

    Type
    Collection
    ... used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation Type Description PI 12253... used with 2nd and 3rd generation lentivirus technologies. Looking for lentiviral plasmids to express ...

  29. Neurodegeneration Research Collection

    Type
    Collection
    ...neurons function. They are also delving into new technologies and resources in order to gain more fundamental...mitochondrial DNA using mitoBEs . Yi et al. Nat Biotechnol. 2023 May 22. See More AAV Viral Preps Find AAV...

  30. CRISPR Plasmids - Tagging

    Type
    Collection
    ...traditional tagging methods. Several different tagging techniques, as well as the plasmids and protocols needed...Förstemann lab has developed a CRISPR tagging technique for use in Drosophila cells that uses PCR to generate...

  32. Plan Your Experiment

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    ...off-target effects. More information on each of these techniques can be found in our blog post CRISPR 101: Validating...genome editing in vitro and in vivo. Nature Biotechnology . 33 (1), 73–80. PMID: 25357182...

  33. Fluorescent Protein Guide: Empty Backbones

    Type
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    ...Center at UCSF / QB3 Wiki Stepanenko et al. : BioTechniques, Vol. 51, No. 5, November 2011, pp. 313–327 ... No. 10, pp.1296-304 Kogure et al. : Nature Biotechnology, May 2006, Vol. 24 No. 5, pp.577-81 Shcherbo...

  34. TALEN Expression Vectors

    Type
    Collection
    ...wild-type FokI nuclease domains ( Sander et al., Nat Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat...

  36. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...dividing cells. CRISPR Activate and Interfere technologies also indirectly modify local chromatin state...

  38. Fluorescent Protein Guide

    Type
    Collection
    ...biosensors. Optogenetics Plasmids | Guide Use light to detect, measure, and control molecular signals, cells,...

  40. CRISPR Plasmids and Resources

    Type
    Collection
    ... CRISPR Plasmids and Resources CRISPR technologies are continously developing and expanding. Addgene...
Showing: 41 - 80 of 794 results