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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 116 results for: PEP

Showing: 51 - 100 of 116 results

  1. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...

  14. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...potential. For example, the best promoter for a given peptide can easily be tested by generating Level 1 TUs ...

  16. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...the surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said...

  19. Optogenetics Guide

    Type
    Guide
    ...Improved light-inducible dimers; Bacterial SsrA peptide is embedded in the LOV2 domain (iLID plasmid), ... target protein is to be sequestered Lumitoxin Peptide toxin for blocking K + (Kv) channels fused to LOV2...allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk Blue-light...

  27. Immunology Research Plasmids and Resources

    Type
    Collection
    ...CCN3, IGFBP9 NPFF neuropeptide FF-amide peptide precursor FMRFAL NPPA natriuretic peptide precursor A ANF...natriuretic peptide precursor B BNP NPPC natriuretic peptide precursor C CNP NPR1 natriuretic peptide receptor...class I polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related...catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase, catalytic, gamma polypeptide PI3CG, PI3K..., FGFB, HBGF-2 FPR1 formyl peptide receptor 1 FMLP, FPR FPR2 formyl peptide receptor 2 ALXR, FMLP-R-II...gonadotropin, beta polypeptide CGB3, hCGB CGB1 chorionic gonadotropin, beta polypeptide 1 - CGB2 chorionic... gonadotropin, beta polypeptide 2 - CGB5 chorionic gonadotropin, beta polypeptide 5 HCG, MGC119822 CGB7...

  28. Pouring LB Agar Plates

    Type
    Protocol
    ...powder consisting of: 5.0 g yeast extract 10.0 g peptone from casein 10.0 g sodium chloride 12.0 g agar-...

  31. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... A) Schematic of workflow. B) Detail of spacer peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm single ...

  33. Antibody Guide

    Type
    Guide
    ...recognizing a different epitope, bound by short peptide linkers. Diabodies can be used for assembling protein...methods. Capture MS validation This method uses peptide identification by proteomics (Capture MS) to validate...analyzed. Validation relies on size comparison of peptides. Independent antibody validation This method validates...

  34. A Control for All Seasons

    Type
    Blog Post
    ...with its antigen or a small epitope-containing peptide to block the antigen-binding site. The sample is...

  35. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...PV), somatostatin (SST), vasoactive intestinal peptide (VIP), or lysosomal-associated membrane protein...

  36. Antibodies 101: Validation

    Type
    Blog Post
    ...specific, then you expect that the bulk of the peptides identified to be from the target protein. This...

  37. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...glycine- and serine-containing peptide linker (GGSGGS) repeat pET28CLY2 Peptide linker standard consisting...proteins connected via 5 amino acid linkers pET28CLY1 Peptide linker standard consisting of ECFP and EYFP connected...and EYFP connected by 2 GGSGGS repeats pET28CLY3 Peptide linker standard consisting of ECFP and EYFP connected...connected by 3 GGSGGS repeats pET28CLY4 Peptide linker standard consisting of ECFP and EYFP connected by... by 4 GGSGGS repeats pET28CLY5 Peptide linker standard consisting of ECFP and EYFP connected by 5 GGSGGS...GGSGGS repeats pET28CLY6 Peptide linker standard consisting of ECFP and EYFP connected by 6 GGSGGS repeats...repeats pET28CLY7 Peptide linker standard consisting of ECFP and EYFP connected by 7 GGSGGS repeats pET28CLY8...

  41. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... Human Serine Phosphopeptide Library described in: Encoding human serine phosphopeptides in bacteria for...serine phosphorylation ( Rinehart Human Serine Phosphopeptide Library ). The phosphorylation sites (“phosphosites...purifying the phosphosite library as GST-fusion peptides (“mode #1” expression). The mode #1 phosphosite...experiments in need of reference spectra or phosphopeptide standards for quantitative assessments. The...detection of phosphorylation-dependent protein-peptide interactions (“mode #2” expression, Barber et al...Profiling Using a Proteome-wide Serine-Oriented Human Peptide Library. Barber KW, Miller CJ, Jun JW, Lou HJ, ...

  42. p53 Pathway

    Type
    Collection
    ...apoptosis-related cysteine peptidase CASP8 Caspase 8, apoptosis-related cysteine peptidase CASP9 Caspase 9, apoptosis-related...Symbol Name 14-3-3-σ Stratifin Apaf-1 Apoptotic peptidase activating factor 1 ATM ATM serine/threonine kinase...apoptosis-related cysteine peptidase Cyclin B CCNB1 CCNB2 CCNB3 Cyclin B1, 2, or 3 Cyclin D CCND1 CCND2... PAG608 Zinc finger, matrin-type 3 PAI Serpin peptidase inhibitor, clade E (nexin, plasminogen activator...

  43. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... pii: 56469. Edwin Chapman Neuropeptides GRAB biosensors for neuropeptides VIP, NPY, CCK, NTS, UCN, SST... orexin neuropeptides A genetically encoded sensor for in vivo imaging of orexin neuropeptides. Nat Methods...based on computationally redesigned calmodulin-peptide pairs. Chem Biol. 2006 May;13(5):521-30. Amy Palmer... selective and sensitive genetically encoded neuropeptide sensors. Science. 2023 Nov 17;382(6672):eabq8173...exploiting a temperature-responsive elastin-like polypeptide. Sci Rep. 2021 Aug 13;11(1):16519. Takeharu Nagai...

  44. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...encoding fluorescent proteins tagged with genes or peptides with known subcellular localization patterns. ... (1-22 aa) import sequence and COX VIII signal peptide dL5 FAP; mCerulean3 Marcel Bruchez 44385 pLV-mitoGFP...KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisome SKL tripeptide, peroxisome transport signal dL5 FAP; mCerulean3...mRFP-Clc Clathrin-coated vesicles Clathrin, light polypeptide mRFP Ari Helenius 70217 pEGFP-GFP11-Clathrin ...Clathrin-LCa-EYFP Clathrin-coated vesicles Clathrin, light polypeptide EYFP Xiaowei Zhuang 14437 mRFP-Rab5 Early endosomes...

  45. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...IgG2a 188173 Anti-NPY2R/Neuropeptide Y receptor type 2 [L119/134R] NPY2R/Neuropeptide Y receptor type 2 Human...Parvalbumin Rat Mouse IgG2b 206639 Anti-Neuropeptide Y [L115/13R-2b] Neuropeptide Y Human Mouse IgG2b 206640 Anti-VGAT... 211594 Anti-NPY2R/Neuropeptide Y receptor type 2 [L119/106R-2b] NPY2R/Neuropeptide Y receptor type 2 ...Rat Mouse 190505 NPY/Neuropeptide Y scFv [L115/13] L115/13 scFv 2t NPY/Neuropeptide Y Human Mouse 190506...mouse)/R. norvegicus (rat) rat IgG2a 227066 NPY/Neuropeptide Y (Homo sapiens) recombinant mouse monoclonal...)/R. norvegicus (rat) rat IgG2a 227067 NPY2R/Neuropeptide Y receptor type 2 (Homo sapiens) recombinant...

  46. Bacterial Expression Systems

    Type
    Collection
    ... cleavage sites. Other protein tags and signal peptides are used to enhance protein solubility and to ...Commonly used tags, cleavage sites, and signal peptides include: Epitope tags: 6xHis, Flag, Strep II, ...protease, factor Xa, enterokinase, thrombin Signal peptides for periplasmic localization: PelB, MalE, OmpA...Methylobacterium extorquens Christopher Marx 122635 pPEPZ-Plac Plac Lactose/IPTG Streptococcus pneumoniae ...

  47. Ras Pathway

    Type
    Collection
    ...2 like 2 NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 PAK PAK1 PAK2 PAK3...catalytic and regulatory subunits - Class I PIN1 Peptidylprolyl cis/trans isomerase, NIMA-interacting 1 PLXNB1...RB1 Retinoblastoma 1 RCE1 Ras converting CAAX endopeptidase 1 RHEB Ras homolog enriched in brain RHO RHOA...

  48. Antibody Guide

    Type
    Collection
    ...recognizing a different epitope, bound by short peptide linkers. Diabodies can be used for assembling protein...methods. Capture MS validation This method uses peptide identification by proteomics (Capture MS) to validate...analyzed. Validation relies on size comparison of peptides. Independent antibody validation This method validates...

  49. CRISPR Plasmids and Resources

    Type
    Collection
    ...activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor peptide, inhibits...
Showing: 51 - 100 of 116 results