We narrowed to 372 results for: BRE

...along the way. Cas3 initiates a final double-strand break, either due to a boundary defined by anti-CRISPR...running into other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair ...

...double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand DNA breaks. Prime Edit...

...precisely editing DNA bases without inducing a DNA break.... single base pair edits without double stranded breaks. Cytosine base editors are created by fusing Cas9...

... generation time (~3 months), ease of care and breeding, and transparency of the embryo and larvae, it...-binary expression system for use in zebrafish - Bret Pearson Lab MAZe: a tool for mosaic analysis of ...

...acceptor for Bioluminescence Resonance Energy Transfer (BRET) . Browse Addgene's Luciferase Plasmid Collection...Promega Plasmid Collection to find related tools for BRET assays. References Bajar, B. T., Wang, E. S., Zhang...

...13. pii: tpc.20.00241. Markus Schwarzländer NADPH BRET semi-synthetic biosensor measuring the NADPH concentration...vinculin loading. eLife. 2018 Jul 19;7. pii: 33927. Brenton Hoffman Voltage JEDI-2P voltage indicator for two-photon...top Other Reporters ONE-GO Biosensors Kit includes BRET-based biosensors of G proteins activated by G-protein-coupled...

...to Kazutoshi Takahashi and Shinya Yamanaka’s breakthrough development of induced pluripotent stem cells...Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. Brennand iPSCs Cortical or Lower Motor Neurons CRISPR/TALEN...

... specific base pair edits without double-strand breaks Base editor (dCas9 or Cas9 nickase fusions with... a homology template, a large percentage of DNA breaks will be repaired by NHEJ instead of incorporating...

...PI Institution NETRF Project Title Hans Clevers Hubrecht Institute Modeling Neuroendocrine Tumors Using...

...159655 are single guide expressing double stranded break constructs. Plasmids 171098 and 175570 allow conversion...

... this temperature but most antibiotics will not break down at this temperature. Check with your antibiotic...

...from the chamber. Use the metal spatula to gently break the gel cast open. Use a razor blade to cut the ...

... Solution in a well ventilated area and avoid breathing in the vapors. Workflow Timeline Day 1: Antigen...

... with poor prognosis in many cancers, including breast, colon, ovarian, and liver cancers. mTORC1 is activated...

...request to delivery could be up to 10 weeks. This breaks down as follows: Request 1–2 days Look for the ...

...from the stable cell pool by trypsinization and break up any cell clumps into individual cells by passing...

... 293T cell line in the presence of 10.0 μg/mL polybrene. This ensures that any loss of titer associated...

...design enables tracking of viral transduction. See Meerbrey et al. (2011) for additional variants. Stephen...

...weakness, difficulty speaking, swallowing, and breathing, and often results in death within 3–5 years from...

...strains. **AAV1-X1 has not been tested in rats. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory...