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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 376 results for: BRE

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Showing: 21 - 40 of 376 results

  1. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ... dCas9 fused to FokI for creating double-strand breaks (DSBs)....When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence. Two unique gRNAs, ...

  2. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...along the way. Cas3 initiates a final double-strand break, either due to a boundary defined by anti-CRISPR...running into other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair ...

  3. CRISPR Plasmids and Resources

    Type
    Collection
    ...double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand DNA breaks. Prime Edit...

  4. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...precisely editing DNA bases without inducing a DNA break.... single base pair edits without double stranded breaks. Cytosine base editors are created by fusing Cas9...

  5. Zebrafish Plasmid Collection

    Type
    Collection
    ... generation time (~3 months), ease of care and breeding, and transparency of the embryo and larvae, it...-binary expression system for use in zebrafish - Bret Pearson Lab MAZe: a tool for mosaic analysis of ...

  6. Fluorescent Proteins: FRET

    Type
    Collection
    ...acceptor for Bioluminescence Resonance Energy Transfer (BRET) . Browse Addgene's Luciferase Plasmid Collection...Promega Plasmid Collection to find related tools for BRET assays. References Bajar, B. T., Wang, E. S., Zhang...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...13. pii: tpc.20.00241. Markus Schwarzländer NADPH BRET semi-synthetic biosensor measuring the NADPH concentration...vinculin loading. eLife. 2018 Jul 19;7. pii: 33927. Brenton Hoffman Voltage JEDI-2P voltage indicator for two-photon...top Other Reporters ONE-GO Biosensors Kit includes BRET-based biosensors of G proteins activated by G-protein-coupled...

  8. Plasmids for Stem Cell Research

    Type
    Collection
    ...to Kazutoshi Takahashi and Shinya Yamanaka’s breakthrough development of induced pluripotent stem cells...Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. Brennand iPSCs Cortical or Lower Motor Neurons CRISPR/TALEN...

  9. Plan Your Experiment

    Type
    Collection
    ... specific base pair edits without double-strand breaks Base editor (dCas9 or Cas9 nickase fusions with... a homology template, a large percentage of DNA breaks will be repaired by NHEJ instead of incorporating...

  10. Pouring LB Agar Plates

    Type
    Protocol
    ... this temperature but most antibiotics will not break down at this temperature. Check with your antibiotic...

  13. NETRF

    Type
    Collection
    ...PI Institution NETRF Project Title Hans Clevers Hubrecht Institute Modeling Neuroendocrine Tumors Using...

  15. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...159655 are single guide expressing double stranded break constructs. Plasmids 171098 and 175570 allow conversion...

  16. mTOR Pathway

    Type
    Collection
    ... with poor prognosis in many cancers, including breast, colon, ovarian, and liver cancers. mTORC1 is activated...

  17. AAV Packaged on Request

    Type
    Collection
    ...request to delivery could be up to 10 weeks. This breaks down as follows: Request 1–2 days Look for the ...

  18. Resolute Plasmid Collection

    Type
    Collection
    ...Teoh, S. T., Leippe, P., Onea, G., Pfeifer, M., Kohlbrenner, M., Chang, L., Selzer, P., Reinhardt, J., Digles...

  19. Viral Production

    Type
    Collection
    ... 293T cell line in the presence of 10.0 μg/mL polybrene. This ensures that any loss of titer associated...

  20. Lentivirus Plasmids

    Type
    Collection
    ...design enables tracking of viral transduction. See Meerbrey et al. (2011) for additional variants. Stephen...
Showing: 21 - 40 of 376 results