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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 381 results for: BRE

Showing: 21 - 40 of 381 results

  1. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...delivered directly to the brain parenchyma or subretinal space Serotypes The following serotypes were ...enhanced transduction of the retina following subretinal administration and enhanced transduction and ...transduction and lateral spread in the retina following subretinal injection. It was identified by John Chiorini...transduction and lateral spread in the retina following subretinal injection as compared to the parental AAV44.9...

  2. Lentiviral Prep Service

    Type
    Collection
    ... generate double-stranded breaks ( DSBs or cuts ) or single-stranded breaks ( nicks ). ID Name Insert ...pooled libraries 92379 Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa...

  3. Pouring LB Agar Plates

    Type
    Protocol
    ... this temperature but most antibiotics will not break down at this temperature. Check with your antibiotic...

  6. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... Lgi1 Mouse Mouse IgG2a 177528 Anti-Brevican [N294A/10R] Brevican Rat Mouse IgG2a 177529 Anti-GluN1 [N308...ARHGAP4 Mouse Mouse IgG2a 190309 Anti-Brevican [N294A/6] Brevican Rat Mouse IgG2a 190311 Anti-NSD1 [N312...Synaptotagmin-7 Mouse Mouse IgG2a 220429 Brevican [N294A/10R-2b] Brevican Rat Mouse IgG2a 220430 BAF53b [N332B...external) Human Mouse 206772 Brevican scFv [N294A/6] N294A/6 scFv Brevican Rat Mouse 206773 GluN3A/NR3A...

  8. Deisseroth INTRSECT Collection

    Type
    Collection
    ... pAAV-nEF-Con/Fon-bREACHes-EYFP Cre AND Flp No 137146 pAAV-nEF-Con/Foff 2.0-bREACHes-EYFP Cre AND NOT ...NOT Flp FRT/F5 No 137147 pAAV-nEF-Coff/Fon-bREACHes-EYFP Flp AND NOT Cre No 137158 pAAV-nEF-ChRmine-mScarlet...

  9. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ... dCas9 fused to FokI for creating double-strand breaks (DSBs)....When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence. Two unique gRNAs, ...

  10. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Cas9 enzymes designed to introduce a double-strand break (DSBs) at a specific location based on a co-expressed... gRNA plasmids, the cell may instead repair the break using homology-directed repair (HDR). HDR is much...

  11. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...along the way. Cas3 initiates a final double-strand break, either due to a boundary defined by anti-CRISPR...running into other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair ...

  12. CRISPR Plasmids and Resources

    Type
    Collection
    ...double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand DNA breaks. Prime Edit...

  13. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...precisely editing DNA bases without inducing a DNA break.... single base pair edits without double stranded breaks. Cytosine base editors are created by fusing Cas9...

  14. Zebrafish Plasmid Collection

    Type
    Collection
    ... generation time (~3 months), ease of care and breeding, and transparency of the embryo and larvae, it...-binary expression system for use in zebrafish - Bret Pearson Lab MAZe: a tool for mosaic analysis of ...

  15. Plasmids for Stem Cell Research

    Type
    Collection
    ...to Kazutoshi Takahashi and Shinya Yamanaka’s breakthrough development of induced pluripotent stem cells...Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. Brennand iPSCs Cortical or Lower Motor Neurons CRISPR/TALEN...

  16. Plan Your Experiment

    Type
    Collection
    ... specific base pair edits without double-strand breaks Base editor (dCas9 or Cas9 nickase fusions with... a homology template, a large percentage of DNA breaks will be repaired by NHEJ instead of incorporating...

  17. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...13. pii: tpc.20.00241. Markus Schwarzländer NADPH BRET semi-synthetic biosensor measuring the NADPH concentration...vinculin loading. eLife. 2018 Jul 19;7. pii: 33927. Brenton Hoffman Voltage JEDI-2P voltage indicator for two-photon...

  18. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ... DNA lesion results from breakage of both DNA strands - a double-strand break (DSB). DSBs can be caused...double-strand break repair (DSBR), synthesis-dependent strand-annealing (SDSA), and break-induced repair... homology directed repair fix DNA double-strand breaks?  In eukaryotic cells, repair of DSBs occurs primarily...have these central steps: The 5’ DNA end of the break is resected by nucleases to create a ssDNA 3’ overhang...covered in detail here. Classical double-strand break repair (DSBR) In the classical DSBR pathway, the...ligated to fill the gap, thus completing SDSA.   Break-induced repair (BIR) pathway BIR is characterized... it has biological importance for the repair of breaks that lack a second end to enable DSBR or SDSA. ...

  19. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...under one of two broad classifications: inbred or outbred. Inbred strains share a common genetic background...of different genome editing techniques. Inbred and outbred strains Mouse strains used in research can...manipulation. Common inbred strains include C57BL/6, Balb/c, and 129. Conversely, outbred strains were originally... genetically diverse gene pool. Outbred strains are strategically bred to maintain maximum genetic heterozygosity...helps improve the reproducibility of experiments. Inbred mice are particularly useful for understanding ...the genetic diversity of the human population. Outbred strains of mice are useful for comparing dominant...not useful for genetic engineering. Examples of outbred strains include Swiss Webster and Harlan (Hsd) ...

  20. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...PMC5171807. 6. Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform for the analysis of protein-protein...known as bioluminescent resonance energy transfer (BRET). The fluor then emits light at a tissue-penetrating...infection of host cells. The fluorescent moiety of the BRET reporter was used to sort successfully infected/...substrate for NanoLuc luciferase. Both GpNLuc and OgNLuc BRET reporters produced bright signals without exogenous...emission near 600 nm – a clear demonstration of why a BRET reporter can improve deep tissue imaging. The constructs...Chu, J., et al. (4), likewise, wanted to produce a BRET probe for imaging. These authors set out to design...Enhanced Nano-Lanterns to other cellular proteins. BRET-based biosensors utilizing NanoLuc® luciferase Many...
Showing: 21 - 40 of 381 results