We narrowed to 958 results for: ache

...Cas9 nuclease form a complex with each individual, unique crRNA. (4) Each crRNA:tracrRNA:Cas9 complex seeks...Doyon Y. 2015. A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells...722-36. PMID: 26411297 Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. ...

...for surface proteins and use enzymatic binding to attach substrate Control plasmids with a recognizable ...expressed and purified as described previously in Stachelhaus et al. (1998) References George N, Pick H, Vogel...J Am Chem Soc. 126(29):8896-7. PMID:15264811 Stachelhaus T, Mootz HD, Bergendahl V, Marahiel MA.1998. ...

...multiple isoforms or subunits, individual links to each gene page are provided below. Color is used for ...multiple isoforms or subunits, individual links to each gene page are provided below. Symbol Name 14-3-3...early ageing-associated phenotypes. Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann ...

...amino acid repeats. These repeats only differ from each other by two amino acids, their repeat-variable ... still determining the importance of context for each TAL effector within an array, but early studies ...Addgene’s history. Dr. Keith Joung’s lab at Massachusetts General Hospital also recently released a TALEN...

...using standard methods that have been optimized for each specific virus in order to generate high quality...ddPCR are generally 2–3 fold higher than those achieved using the standard qPCR method. Titering by the...The specific QC experiments performed varies for each viral lot. To learn which specific QC experiments...

...key modifications to the standard translation machinery are required: The ncAA is generally supplied in...independently of the host cell's natural genetic machinery. The orthogonal aaRS enzyme should not recognize...charge the tRNA with a different amino acid. To achieve these modifications, two plasmids are generally...incorporated using the existing protein translation machinery. A synthetase plasmid expressing the engineered...

...body during development. Unlike other cell types, each stem cell has the potential to either remain a stem...use. Since 2006, scientists have developed many approaches to generate iPSCs. The generation of iPSCs is...Xeno-Free Culture Conditions: A Clinically Compliant Approach. Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014...

... pooled collection, can be purchased at Addgene. Each transcription factor isoform has a unique 24 bp ... human transcription factor open reading frames. Each isoform is uniquely barcoded with a 24 bp sequence...

...mutant, knockdown, and overexpression constructs. Each collection page listed below organizes the plasmids...extremely important for classification and treatment. On each pathway page, click on a component to find plasmids...

...titers are listed on each item's material page. Actual titers are reported with each shipment. If you can't...

...need to get your experiments started more quickly. Each viral vector is produced from a cargo plasmid in... Vector Core catalog items. Virus-associated DNA Each viral vector service request will also include virus-associated...

...single round of viral entry and infection. This approach allows researchers to safely study mechanisms ...SARS-CoV-2 Spike protein and you will find examples of each in Addgene's collection: HIV-based lentiviral particles...

... Editing in Caenorhabditis elegans. A modified approach has also been developed by the Fire laboratory...Other Resources Addgene Blog Posts An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency Fluorescent...

...titers are listed on each item's material page. Actual titers are reported with each shipment. For more ...

... terminals. 1 Liqun Luo 60658 pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato EF1a-driven, Flp-dependent Flp-dependent...Flp-dependent expression of the voltage-gated Na+ channel mNaChBac and (physically separate) tdTomato. 8 Massimo ...

...lab at the University of Florida. References for each serotype can be found below under Citations. AAV2...Plasmid Information The plasmid below is available in each of the above serotypes and buffers. Please click...

...multiple subunits or isoforms, individual links to each gene page are provided below. Color is used for ...multiple subunits or isoforms, individual links to each gene page are provided below. Symbol Name AKT AKT1...

...is driven by the strong CAG promoter or can be achieved by in vitro mRNA synthesis from the T7 promoter...Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination vectors can be used to create...

...fungi, protists, and viruses. Developed by the Joachim Wittbrodt and Juan L. Mateo (Link opens in a new...

... of this page. For more in depth descriptions of each serotype, check out Addgene's blog on Systemic Capsids... Capsids . To review performance data of each of these serotypes, please refer to Addgene’s Data Hub and...