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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 376 results for: bre

Showing: 21 - 40 of 376 results

  1. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...doesn't have the ability to induce double-stranded breaks like the wildtype Cas9 does. There are a few references... add an additional 'G'. Where are double strand breaks (DSBs) induced, compared to where the target site...homology arm needed? How should the double strand break (DSB) be positioned relative to where the homology... are less than 15bp away from the double strand break site. Longer distances will also work but the efficiency...

  2. Lentiviral Prep Service

    Type
    Collection
    ... generate double-stranded breaks ( DSBs or cuts ) or single-stranded breaks ( nicks ). ID Name Insert ...pooled libraries 92379 Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa...

  3. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... Lgi1 Mouse Mouse IgG2a 177528 Anti-Brevican [N294A/10R] Brevican Rat Mouse IgG2a 177529 Anti-GluN1 [N308...ARHGAP4 Mouse Mouse IgG2a 190309 Anti-Brevican [N294A/6] Brevican Rat Mouse IgG2a 190311 Anti-NSD1 [N312...Synaptotagmin-7 Mouse Mouse IgG2a 220429 Brevican [N294A/10R-2b] Brevican Rat Mouse IgG2a 220430 BAF53b [N332B...external) Human Mouse 206772 Brevican scFv [N294A/6] N294A/6 scFv Brevican Rat Mouse 206773 GluN3A/NR3A...

  4. Deisseroth INTRSECT Collection

    Type
    Collection
    ... pAAV-nEF-Con/Fon-bREACHes-EYFP Cre AND Flp No 137146 pAAV-nEF-Con/Foff 2.0-bREACHes-EYFP Cre AND NOT ...NOT Flp FRT/F5 No 137147 pAAV-nEF-Coff/Fon-bREACHes-EYFP Flp AND NOT Cre No 137158 pAAV-nEF-ChRmine-mScarlet...

  5. Pouring LB Agar Plates

    Type
    Protocol
    ... this temperature but most antibiotics will not break down at this temperature. Check with your antibiotic...

  9. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ... dCas9 fused to FokI for creating double-strand breaks (DSBs)....When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence. Two unique gRNAs, ...

  10. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Cas9 enzymes designed to introduce a double-strand break (DSBs) at a specific location based on a co-expressed... gRNA plasmids, the cell may instead repair the break using homology-directed repair (HDR). HDR is much...

  11. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...along the way. Cas3 initiates a final double-strand break, either due to a boundary defined by anti-CRISPR...running into other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair ...

  12. CRISPR Plasmids and Resources

    Type
    Collection
    ...double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand DNA breaks. Prime Edit...

  13. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...precisely editing DNA bases without inducing a DNA break.... single base pair edits without double stranded breaks. Cytosine base editors are created by fusing Cas9...

  14. AAVED

    Type
    Collection
    ...Speakers and Attendees Friday, September 21 9:00 AM Breakfast and Welcome Melina Fan 9:30 AM Cre and Flpo Katherine...and Combinatorial Strategies Connie Cepko 2:45 PM Break 3:00 PM (Group Discussion) Technologies and Resources...

  15. Zebrafish Plasmid Collection

    Type
    Collection
    ... generation time (~3 months), ease of care and breeding, and transparency of the embryo and larvae, it...-binary expression system for use in zebrafish - Bret Pearson Lab MAZe: a tool for mosaic analysis of ...

  16. Plasmids for Stem Cell Research

    Type
    Collection
    ...to Kazutoshi Takahashi and Shinya Yamanaka’s breakthrough development of induced pluripotent stem cells...Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. Brennand iPSCs Cortical or Lower Motor Neurons CRISPR/TALEN...

  17. Cre-lox system

    Type
    Collection
    ... pCag FlpO-2A-Cre EV FlpO-2A-Cre CAG Mammalian Breunig 130986 pCag SE (Self-excising) FlpO-2A-Cre EV Self-excising... Self-excising FlpO-2A-Cre CAG Mammalian Breunig 131780 pCS2+PIF6CreN CreLite - Red light-inducible Cre...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...13. pii: tpc.20.00241. Markus Schwarzländer NADPH BRET semi-synthetic biosensor measuring the NADPH concentration...vinculin loading. eLife. 2018 Jul 19;7. pii: 33927. Brenton Hoffman Voltage JEDI-2P voltage indicator for two-photon...

  19. NETRF

    Type
    Collection
    ...PI Institution NETRF Project Title Hans Clevers Hubrecht Institute Modeling Neuroendocrine Tumors Using...

  20. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ... DNA lesion results from breakage of both DNA strands - a double-strand break (DSB). DSBs can be caused...double-strand break repair (DSBR), synthesis-dependent strand-annealing (SDSA), and break-induced repair... homology directed repair fix DNA double-strand breaks?  In eukaryotic cells, repair of DSBs occurs primarily...have these central steps: The 5’ DNA end of the break is resected by nucleases to create a ssDNA 3’ overhang...covered in detail here. Classical double-strand break repair (DSBR) In the classical DSBR pathway, the...ligated to fill the gap, thus completing SDSA.   Break-induced repair (BIR) pathway BIR is characterized... it has biological importance for the repair of breaks that lack a second end to enable DSBR or SDSA. ...
Showing: 21 - 40 of 376 results