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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 368 results for: gne

Showing: 21 - 40 of 368 results

  1. Antibody Guide

    Type
    Collection
    ...agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic beads), collect...are two primary methods used, using agarose or magnetic beads, but both rely on the same general strategy...

  2. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... the Funabiki Lab developed a technique called Magnetic Isolation and Concentration (MagIC)-cryo-EM that...enrichment and imaging of proteins captured on magnetic beads (Arimura et al., 2024). In MagIC-cryo-EM...proof-of-concept study) are captured on streptavidin-coated magnetic beads through an assembly of biotinylated SpyTag-SpyCatcher... identified by Topaz software (green circles), magnetic bead (*), and spacers are labeled; scale bar: ...2024) MagIC-Cryo-EM: Structural determination on magnetic beads for scarce macromolecules in heterogeneous...

  3. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ... the state of the RSM. If an attB-attP pair is aligned (sites facing the same direction) on a register...). Alternatively, if an attB-attP pair is anti-aligned (sites facing opposite directions) on a register...recognition sites from the register. If the RSM is designed in such a way that each possible order of inputs...were built with flanking primer binding sites (designed to have 20-30 nt length, ~60 oC annealing temperature...Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014...

  4. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...team was the first to develop and publish a human-designed protein that was not based on any existing protein...got closer and closer to achieving it. Once they designed the protein computationally, the researchers expressed... protein design is possible. Although Top7 was designed simply to be stable and novel, the principles ...Baker Lab and others. The introduction of de novo designed proteins also helped researchers understand more...

  5. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...construct, most often in special E. coli strains designed for protein expression. Cultures are then lysed...and Pichia pastoris pCri also includes plasmids designed for protein expression in systems other than E...other eukaryotes better than E. coli. Plasmids designed for these systems may include a signal peptide...difficult-to-purify membrane proteins. pCri vector pCri-13a, designed for use in B. subtilis, contains the MISTIC (membrane-integrating...

  6. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...cell events Three years ago, Brian Kuhlman’s lab designed photoswitchable dimers by fusing the SsrA peptide...gene is inserted. It is important that primers be designed to minimize the possibility of dimer formation...preposterous. Luckily, the Pierre Neveu laboratory designed a new iterative, chaining-based cloning method...incorporation of a variety of bio-orthogonal UAAs via a re-assigned stop codon (TAG). This polyspecificity enables...translation. This luciferase reporter system was also designed for a short screening time (<4 hours) in order...expression as anticipated. For instance, an aptamer designed to bind a transcriptional activator activated ...loci using validated gRNAs from Addgene and well designed homology directed repair templates (see the supplementary...

  7. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...range and high brightness. The Shu Lab from UCSF designed an improved apoptosis reporter, ZipGFP, which ...prevent binding, the β strands in FlipGFP were redesigned so that they are parallel to each other and thus...fluorogenic reporter toolkit, the Shu Lab also designed a red fluorogenic protease reporter based on superfolder...FlipCherry provides a proof of concept that the designed used in FlipGFP can be applied to other fluorescent...

  8. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ... launched earlier in the year, as well as our redesigned homepage and our new search tool, are the results...per resource category to expose as filters and designed the search engine to return only the filters that... bits of information for a given resource, and designed the search engine to weight search results differently...'s new search Read to learn more about why we redesigned our homepage and mascot ...

  9. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...customized to direct assembly of DNA fragments. When designed correctly, the recognition sites do not appear... scheme is as follows: the gene of interest is designed with Type IIS sites (such as BsaI or BbsI), that...eight amino acids, but Golden Gate assembly can be designed to be scarless. Golden Gate assembly is also less...create TAL effector nucleases. These plasmids were designed to utilize the BsaI and BsmBI Type IIS sites such...

  10. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ... guides used in the screens they analyzed were designed to have a perfect match to the reference genome...Katherine Huang from Broad’s Pattern team, they designed a tool that could analyze CRISPR sgRNAs and report...CRISpick algorithm to prevent guides from being designed that target regions with high genomic variability...scientists to be aware of and actively use tools designed to help reduce or eliminate bias, just like the...

  11. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...most yeast vectors and many mammalian vectors are designed to shuttle between species. Figure 1: Shuttle...host’s replication machinery. A shuttle vector designed for unrelated bacteria should include oris for...target species. Fun fact! Plant vectors are usually designed to shuttle between E. coli and Agrobacterium tumefaciens...image for individual analysis. A shuttle vector designed for this approach in a mammalian cell experiment...

  12. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...reproducibility than others. Unfortunately, most ELNs are designed as closed ecosystems and act as data silos because...RSpace ELN supports reproducibility RSpace was designed in response to requirements that emerged from ... in institutional and lab servers specifically designed to hold large amounts of data. Although it may...Since most ELNs, unlike data repositories, are designed to support confidentiality and limit permissions...

  13. Important Considerations When Using AAVs

    Type
    Blog Post
    ...utilizing chemogenetics (Designer Receptors Exclusively Activated By Designer Drugs, DREADDs) and optogenetics...examining the use of DREADDs (Designer Receptors Exclusively Activated By Designer Drugs) to determine the ...

  14. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...many MoClo kits are designed with specific model organisms in mind, others are designed for flexibility. .... phaffii, then the MoClo YTK Kit specifically designed for S. cerevisiae is at your service (Lee et al... the Multi-Kingdom (MK) Golden Gate system was designed to work in fungi, bacteria, protists, plants, ...

  15. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...Hot Plasmid 2: Magneto 2.0 form Ali Guler's Lab - Control neuronal activity with a magnetic field. Listen...Listen to Our Segment on Magneto 2.0 Hot Plasmid 3: The Human Kinase Domain Constructs Kit from John Chodera's...

  16. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...module vectors, including three designed for dicots and three designed for monocots. Using these gRNA ...Cas9 (or other CRISPR) system that the vector was designed to be used with, such as S. pyogenes, S. aureus...

  17. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays...Expression Vectors This page lists plasmid vectors designed to express nucleases in different cell types. ...

  18. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...CRISPR plasmids that have been designed for use in zebrafish.... Posts The following CRISPR plasmids have been designed for use in zebrafish. Cut Fully functional CRISPR...

  19. CRISPR Plasmids - Parasites

    Type
    Collection
    ...CRISPR expression plasmids that have been designed for use in parasites....CRISPR genome-wide knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding...

  20. Chemogenetics Plasmids

    Type
    Collection
    ... chemogenetic receptors: Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) and Pharmacologically...
Showing: 21 - 40 of 368 results