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We narrowed to 407 results for: expression vector

Showing: 381 - 400 of 407 results

  1. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...full sequencing of the final viral vector preparation. These viral vector preparations were produced with...rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep... Viral Vector Packaging Service AAV University of Florida Serotype...Serotype Testing Panel for the Eye and Brain Viral Vector Packaging Service: University of Florida Serotype...Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part of our Viral Service , Addgene is producing...of our standard viral production , these viral vectors undergo quality control, including AAV titration... in vitro as well as in vivo delivery where the vector is diluted by the physiologic body fluids, such...

  2. CRISPR Guide

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    Collection
    ...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...

  3. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ... nickase plasmids. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants...

  4. Ras Pathway

    Type
    Collection
    ...to permit construction of RAS pathway gene expression vectors for in vitro and in vivo research. The RAS...plasma membrane. Cancer Impact Ras is ubiquitously expressed in all cells and all tissues. Ras can activate...Resources RAS Pathway Clone Collection 2.0: Set of vectors from the Esposito Lab for use with the Gateway®...

  5. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...targeting plasmids. Plasmids are available for expression in mammalian systems, bacteria, and plants. Mammalian...

  6. CRISPR Plasmids - Parasites

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    Collection
    ...CRISPR expression plasmids that have been designed for use in parasites....-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...

  7. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...to introduce a DSB. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants...

  8. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Transposase plasmids. Plasmids are available for expression in mammalian systems and bacteria. Mammalian ...

  9. Control AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Controls Viral Vector Packaging Service: Control...Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These AAV can... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

  10. Genetic Code Expansion

    Type
    Collection
    ...plasmids, as depicted in the figure below: A plasmid expressing the tRNA and its cognate aminoacyl-tRNA-synthetase...codon is often used. The gene of interest can be expressed from a plasmid containing a UAG codon at the place...with a different amino acid. They are typically expressed from a single plasmid, with multiple copies of...make sure that these conditions are true. Always express first with a control reporter gene – GFP for E....mCherry-GFP for mammalian cells. You should also express the protein with and without the ncAA in the media... tRNA gene. ID Plasmid Synthetase Origin ncAA Expression Codon PI 31186 pEVOL-pAzF p-azidohenylalanine...mutated, serB deleted for phosphoserine GCE. For expressing phosphoserine-containing proteins using genetic...

  11. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...termed enhancers — to drive cell type-specific expression. The Allen Institute for Brain Sciences has screened... AAV delivery. The collection includes the top vectors with the highest specificity and brightness, assessed...Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex. Cell Rep. 2021...

  12. Biosensor AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor...Biosensor AAV Browse our AAV that express fluorescent biosensor proteins, including calcium sensors, under... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

  13. Plan Your Experiment

    Type
    Guide
    ...Delivery method Expression System Components of System Application Mammalian expression vector Cas enzyme ...system. Select Expression System To use CRISPR, you will need both Cas9 and a gRNA expressed in your target...to the transient Cas9 expression. The table below summarizes the major expression systems and variables...details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA in a mammalian... in a single lentiviral transfer vector or separate transfer vectors May contain reporter gene (e.g. GFP... or electroporation Transient expression of CRISPR components Expression decreases as RNA is degraded ...gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells expressing both Cas9 and ...

  14. Adenovirus Guide

    Type
    Guide
    ...Guides Adenoviral Vector Guide Adenoviral Vector Guide Jump To: Adenoviral Vectors Vector Production Integration...capacity of rAdV vectors (~6.5 kb for first-generation rAdV vectors) surpasses that of rAAV vectors (~4.7 kb)...Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...adenoviral (rAdV) vectors in order to deliver genetic cargo into cells. Most rAdV vectors are based on adenovirus...Generations First-generation rAdV vectors The first generation of rAdV vectors was developed by removing the...other means. In order to produce rAdV vectors, E1 needs to be expressed by the packaging cell line, normally...

  15. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Guides AAV Vector Guide Adeno-Associated Viral (AAV) Vector Guide Jump To: AAV Vectors Vector Production... genome for viral vector production, rAAV vectors do not carry the genes to express Rep78 and Rep 68 proteins...Read our adeno-associated viral vector guide to learn about AAV vector components, production and common...used as viral vectors for gene therapy applications. This guide contains many viral vector-specific terms...adenovirus, read our Adenoviral Vector Guide . Recombinant AAV Vectors Wild-type AAVs have been modified...Plasmids required for rAAV vector production. Created with BioRender.com. AAV Vector Production Cloning Cloning...laboratory. The cell line used for rAAV vector production needs to express the adenovirus E1 gene, which is ...

  16. Resolute Plasmid Collection

    Type
    Collection
    ... SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors allow a fast and efficient sub-cloning...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression pooled .... Human SLC Overexpression Library 213694 Superti-Furga Inducible barcoded overexpression library of human...

  17. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Gamma-Retroviral Vector Guide Gamma-Retroviral Vector Guide Jump To: Gamma-Retroviral Vectors Vector Production...gamma-retroviral vectors is essentially the same as lentiviral vectors. Many gamma-retroviral vectors have selectable...of Viruses Viral Vector Elements More Viral Vectors 101 topics More Retroviral Vector topics Addgene protocols...long-term expression in a host and low immunogenicity. This guide contains many viral vector-specific ...Gamma-retroviral vectors do not have defined “generations” of plasmids, as lentiviral vectors do. For more...lentiviruses, see Addgene's lentiviral vector guide . Gamma-Retroviral Vectors The genome of gamma-retroviruses...Addgene’s viral vector protocols . Production Using Packaging Cell Lines Gamma-retroviral vectors can be packaged...

  18. Molecular Biology Reference

    Type
    Guide
    ...Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. If you are looking...information. Expression Plasmids - Used for gene expression (for the purposes of gene study). Expression vectors...amount of protein or RNA produced. Expression vectors can drive expression in various cell types (mammalian... the level of insert expression (i.e., a strong promoter directs high expression, whereas weaker promoters...for the transcription process to stop. An expression vector can also include an enhancer sequence which...reducing the expression of an endogenous gene. This is frequently accomplished through expression of an shRNA...combination of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule...

  19. Lentiviral Vector Guide

    Type
    Guide
    ...Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations Vector Production...Lentiviral Vectors Due to their integration and long-term expression of a transgene, lentiviral vectors are ...Viral Vectors 101 topics More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols...lentiviral vectors that can be safely used in laboratory settings. These engineered lentiviral vectors have ...models. Lentiviral vectors can target non-dividing cells, enable stable long-term expression in a host, and...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...gamma-retroviruses, see Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically...

  20. Chemogenetics Guide

    Type
    Guide
    ... Dlx Interneurons EF1a, CAG General expression Targeted expression. Depending on the experiment, an AAV-encoded...which they drive expression. Different AAV serotypes can be used to target AAV vectors to specific cells... our AAV guide . FLEx Vectors are also used to achieve cell-specific expression of AAV-encoded chemogenetic...chemogenetic receptors. FLEx vectors block expression in the absence of Cre and are especially useful in AAV...Want to know more about FLEx vectors? Read our Plasmids 101: FLEx Vectors blog! Download our chemogenetics.... The opsin may excite or inhibit the neurons expressing the LMO, depending on its biophysical properties...more than one domain, both of which need to be expressed. However, neuronal control through PSAMs and LMOs...
Showing: 381 - 400 of 407 results