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  1. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...technically gamma-retroviruses - another, separate member of the retroviral family. While both lentiviruses...Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing and non-...

  2. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...Maybe you’ll need to care for an elderly family member, want to train for a triathlon, finally buy that...laundry, take public transportation, call a cab and remember their own school assignments, the better. In my...

  3. CRISPR 101: Any Base Transversion Editors

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    Blog Post
    ... thereafter, using mouse instead of human AAG (remember, MPG and AAG are the same protein!) (Chen et al...A•T-to-C•G base editing in mammalian cells and embryos. Nature Biotechnology, 42(4), 638–650. https://...

  4. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...interested in working for this lab and/or company. Remember to proofread your cover letter, and if possible... originally written by Maria Soriano-Carot in September 2016 and was updated by Rachel Leeson in May 2024...

  6. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...for Excellence in Molecular Plant Sciences since December 2020. His research focuses on the efficiency of...for Excellence in Molecular Plant Sciences in September 2019 as a Ph.D. candidate. Her research focuses...

  7. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...cleaving genomic DNA in mammalian cells and zebrafish embryos, and they can be used to modify genomic loci that...SpCas9 (Amrani et al., 2018). When choosing a Cas9, remember to check that your tracrRNA and crRNA (or synthetic...

  8. Hot Plasmids - August 2020

    Type
    Blog Post
    ...with plasmids encoding FPs tagged with the plasma membrane targetin sequence derived either from p63(1-29...non-specific labeling. For labeling the plasma membrane, they tested different target sequences for improved...

  9. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post
    ...Members of the bacterial world produce an assortment toxins to claim territory or kill competing microorganisms... (hok), a long-living toxin that damages cell membranes; Suppression of killing (sok), a short-lived RNA...

  10. Using Video to Share Your Science: We Share Science

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    Blog Post
    ...https://youtu.be/EHHhiawIdWM Saving salmon, one embryo at a time – A video by LeeAnne Thayer, a Ph.D. ...University of Maine, about her research on plummeting embryo survival rates. Video on We Share Science: http...

  11. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post
    ...harboring this mutation are deficient in outer membrane protease VII, which reduces proteolysis of the...pLemo (CamR) Expression of toxic, insoluble, or membrane proteins  T7 Express   IPTG-inducible expression...

  12. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post
    ...cells.   GFP is big GFP is a 28 kDa protein that resembles a cylinder with a length of 4.2 nm and a diameter...Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells." Science 296.5569 ...

  13. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College of Medicine. Several... guest blogger! Daria Shcherbakova is a faculty member at Albert Einstein College of Medicine. Her expertise...

  15. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...empathy, a willingness to listen, and an ability to remember the right stories at the right time. Like many...morefamousquotes.com A good listener absorbs and remembers and makes the teller feel heard. I am not always...

  16. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...recommendations.  As you optimize your protocol, always remember to include controls to show that your antibodies...sample).  When choosing a primary antibody, first, remember that antibodies recognize very specific 3-dimensional...

  17. Thoughts on the Future of Research 2015 Boston Symposium

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    Blog Post
    ... Symposium 2015. I personally participated as a member of the Career Paths Panel on the second day of ...people from all backgrounds are truly included as members of their research communities and are treated equitably...

  18. A Control for All Seasons

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    Blog Post
    ...and equal protein transfer from the gel to the membrane. A loading control is critical for data interpretation.... Always probe the loading control on the same membrane that the target protein was measured on.    Flow...

  20. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...contributed by guest blogger, Addgene Advisory Board member, and Institute Scientist at the Broad Institute.... While this seems obvious, it is important to remember that the same is true when designing gRNAs for...
Showing: 401 - 420 of 615 results