We narrowed to 583 results for: RET
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TypeBlog Post...also happen at the state and local level. The big secret about science policy that I didn’t learn until ...
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A History of Genome Engineering in Popular Culture
TypeBlog Post...install all possible point mutations, delete short stretches of DNA and even insert new DNA at the target genomic... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...simultaneously recorded the electrical impulses of discrete subpopulations of this brain area, demonstrating... -
Antibodies 101: Introduction to Antibodies
TypeBlog Post...variable region of an antibody does not recognize the entirety of the target protein: instead, it recognizes ... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...process called Fluorescence Resonance Energy Transfer (FRET). Figure 1: Emission spectra and respective... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...Pitfalls of Working With Fluorescent Proteins Use FRET to Monitor Protein-Protein Interactions Resources... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...give you experimental results that are easier to interpret. The light intensity used to activate the PA-FP... -
Which Fluorescence Microscopy Techniques is Best for Me?
TypeBlog Post...imaging can image thick scattering samples in their entirety, something that is not possible via traditional... -
Selecting Your Plasmid Purification Kit
TypeBlog Post...kit. Do you want eco-friendly options? It’s no secret that labs produce a lot of waste. If you are looking... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...define a "specialty occupation" as requiring theoretical and practical application of a body of highly... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post...notable tools allow the user to save the results and return later or share them. Community exclusivity: Different... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...https://doi.org/10.1128/mbio.00019-20 Singh AK, Carette X, Potluri L-P, Sharp JD, Xu R, Prisic S, Husson... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post... of ~50% and confirmed that the fusion protein retained the PAM properties of the individual Cas9. They... -
Lentiviral Vector Guide
TypeGuide... Versus Gamma-Retrovirus Lentiviruses and gamma-retroviruses fall under the Retroviridae family. The genome...Glossary Lentiviruses are a type of retrovirus and belong to the Retroviridae family. Infection with lentiviruses...variety of cell types than retroviruses. Both lentiviruses and gamma-retroviruses use the same packaging ...packaged by gamma-retroviral packaging systems, and vice versa. For more about gamma-retroviruses, see Addgene's..., leading to disease. Lentivirus A retrovirus from the Retroviridae family. Characterized by long incubation...BIV), and feline immunodeficiency virus (FIV). Retroviruses are partially characterized by their ability...of viruses in this family is made of RNA. This retroviral RNA is reverse transcribed into DNA before being... -
Adeno-associated virus (AAV) Guide
TypeGuide...variant able to display neuronal retrograde transport, AAVrg. Retrograde transport refers to the direction...AAV6, AAV8 Photoreceptor cells AAV2, AAV5, AAV8 Retinal pigment epithelium AAV1, AAV2, AAV4, AAV5, AAV8.... Browse Addgene’s in-stock viral preps . AAV retrograde (AAVrg) Directed evolution has also been employed...treat a diversity of diseases such as inherited retinal disease, spinal muscular atrophy, hemophilia A ...capable of both anterograde (from soma to axon) and retrograde (from axon to soma) transport. Many rAAV vectors... use with rabies virus (RABV) in monosynaptic retrograde tracing of neurons. In this system, the rAAV ...2016). A designer AAV variant permits efficient retrograde access to projection neurons . Neuron, 92 (2)... -
CRISPR Guide
TypeGuide...coding sequence ~1 kb shorter than SpCas9 while retaining the same basic function. Its size allows it to...AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells that are poorly...Cas9 Fusion Tools dCas9 cannot cleave DNA, but it retains the ability to find specific DNA targets through...specificity, and editors carrying an ADAR truncation that retain RNA editing capabilities but are small enough to...23979020 Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W.,...., Terns, M. P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (...: 32572269 Charpentier, M., Khedher, A. H. Y., Menoret, S., Brion, A., Lamribet, K., Dardillac, E., Boix... -
Molecular Biology Reference
TypeGuide...plasmids through cell division. To ensure the retention of plasmid DNA in bacterial populations, an antibiotic...these conditions, there is a selective pressure to retain the plasmid DNA, despite the added replication ...to create viral particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that can infect... -
Antibody Guide
TypeGuide... Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects... your samples using permeabilization, antigen retrieval, and/or sectioning. Block to reduce non-specific...upwards of twenty. Laser and software capacity theoretically allow for roughly fifty different fluorophore... -
Cloning
TypeGuide...final product, with no undesired sequence ("scar") retained. A disadvantage of type IIS assembly cloning systems...restriction site. Because of the relatively long stretches of base pairing in the annealed product, ligation... -
Chemogenetics Guide
TypeGuide...chemogenetic access to optogenetic tools, they also retain the ability to be activated by standard optogenetics...chemogenetic control in a single molecule driven by FRET-modified bioluminescence. Neurophotonics. 11:021005...