We narrowed to 844 results for: Tat

...operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA). In normal conditions, the TetR protein ...tetracycline-controlled transactivator (fusion of mutated TetR with VP16 transcriptional activation domain...Tet-On) In 1995, Gossen et al. demonstrated that mutating the amino acid residues of TetR that were important... lineages and transcriptional and proliferative state of each cell in a population. Proliferation history...tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity . Proc Natl...

...of enhancers and non-coding RNAs in zebrafish - Tatjana Sauka-Spengler Lab Lineage Tracing Cell lineage... typically relies on genetic markers, somatic mutations, or labeling with fluorescent proteins to visualize...dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch Lab. A vertebrate...potential cancer drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler Lab. A genetic binary system for...

...(Link opens in a new window) for targeted gene mutation. Combined fluorescent seed selection and multiplex...planta via ratiometric Bimolecular Fluorescence Complementation (rBiFC) and Förster Resonance Energy Transfer...MoBiFC Kit Modular bimolecular fluorescence complementation (MoBiFC) toolkit for analyzing protein-protein...resource composed by a collection of databases, computational and experimental resources that relate to the...

...Domain Deep Mutation Scanning Library Screening Jesse Bloom Library of Spike protein RBD mutations for comprehensive...cleaves precursor proteins to a biologically active state. The SARS-CoV-2 S protein contains a potential cleavage... Starr Libraries of variant Spike protein RBD mutations for comprehensive mapping. Other Resources Protocols...

...CRISPResso CRISPResso2 is a robust and easy-to-use computational pipeline for the analysis of genome editing ...experiments. It enables rapid and intuitive interpretation of results produced by amplicon sequencing.... opens in a new window) A tool that identifies putative target sites for S. pyogenes Cas9, S. aureus Cas9...off-targets, target's genomic location, and genome annotation. Available genomes are human (hg19 & hg38), mouse...

...endogenous TAG codons mutated to TAA, RF1 (prfA) deleted, fabR spontaneously mutated, serB deleted for phosphoserine...eukaryotic cells. Many of these sets have been mutated and screened through directed evolution to charge...RF1 function removed, MutS restored so decreased mutation rate George Church 68306 C321.ΔA all TAG sites... to UAA. mutS+, λ-, ΔybhB-bioAB, ΔprfA, ΔserB mutations for improved yield and growth Jesse Rinehart 197655...

...for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential for regulating...growth of the cell. p53 p53, the most frequently mutated gene in human cancer, is a transcription factor...labs for characterization of all possible p53 mutations. Resources New Tool for Lineage Tracing: The ClonTracer...

...imaging tools in this blog post . Far-Red Protein Excitation (nm) Emission (nm) Brightness Plasmids mCardinal...Crimson pAAV-CAG-E2-Crimson Near-Infrared Protein Excitation (nm) Emission (nm) Brightness Plasmids emiRFP670... 720 5.8 piRFP720-N1 Photoactivatable Protein Excitation (nm) Emission (nm) Activation (nm) Brightness...

...Reconstruction by Iterative Staining with Molecular annotations (PRISM) is a set of methods and molecular tools...barcodes allow for efficient machine-learning segmentation of neurons and proofreading of neuron reconstructions...small to limit the amount of bias in packaging representation. To read out the barcodes, each protein tag...

...installing targeted insertions, deletions, and point mutations using a prime editing guide RNA (pegRNA). As with...tools are available: PE2 — introduction of five mutations in RT enzyme PE3 — PE2 plus additional sgRNA PE4...additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can be used...

...steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation letter DNA...pricing for viral vector preps, which includes MTA facilitation, DNA amplification, and high-quality quality...

...GTPases have an active (GTP) and inactive (GDP) state, regulated by the addition and removal of a phosphate...regulating the growth of the cell, however when mutations occur in the Ras gene it can be stuck in a constitutively...PubMed PMID: 17428555 . RAS signaling pathways, mutations and their role in colorectal cancer. Zenonos K...

...dominant manner, are associated with known genetic mutations in one of the following genes: SOD1, C9ORF72, ... huntingtin (HTT) gene. An individual with the mutated HTT gene will have huntingtin proteins with abnormally...-onset forms, a fraction of these are due to a mutation in one of three different inherited genes: Presenilin...

...precise gene editing. These edits can include mutations seen in human patients, protein tags, or loxP/...off-target activity due to rationally designed mutations. Researchers have expanded the CRISPR field beyond... DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res...

...investigators who will use state-of-the-art experimental and computational approaches to model, predict...

...SUMO, GST, MBP, CBP, S-tag, TAP tag, TRX, or V5) Mutations Wild type (wt) Codon-optimized expression (recode...making pseudovirus (Ctrunc) ID Plasmid Description Mutations Tags Industry Additional Addgene Resources Blog...

...approximately 450 identified members. Many SLCs have mutations associated with disease and could become targets...and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination of metabolomics...

...or to knockdown genes with potentially harmful mutations in therapeutic settings. As a tool in mammalian...RISC includes argonaute protein 2 (Ago2), which facilitates cleavage of the target. RNAi using siRNA follows...

...ZFNs) are effective tools for making targeted mutations in zebrafish. With support from the NIH and guidance...hydroxylase (dbh) OZ525 and OZ526 OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527...

...fragments can be used in experiments such as immunoprecipitation and super-resolution microscopy. Two commonly...amber codons in scFvs A new expression vector facilitating production and functional analysis of scFv antibody...

...integrates these signals to determine the nutritional state of the cell and regulates the switch from anabolic...multiple familial cancers are caused by inactivating mutations in negative regulators of mTORC1. The hyperactivation...

...i.e., genome containing) AAV particles within representative vector preparations was quantified with electron...for viral production are propagated in the endA -mutated NEB Stable strain of E. coli . In addition, plasmids...

...Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden Citation Information When using the Caltech serotypes in...L4 - AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For...

...Rat Mouse IgG2a 140065 Anti-SCG10/Stathmin-2 [L5/1R] SCG10/Stathmin-2 Rat Mouse IgG2a 140066 Anti-Co-Rest...83R] BAF53a Human Mouse IgG2a 206558 Anti-ATAT1 [N347/42R] ATAT1 Human Mouse IgG2a 206559 Anti-Foxi2 [N349...distinct IgG subclass from the conventional mAb to facilitate multiplex labeling with subclass-specific secondary...

...Purification Miniprep, phenol-chloroform extract, and precipitate DNA DNA Quantification Measure DNA concentration...

...making a small change in a plasmid (such as point mutations). Generate DNA segments by PCR. Run PCR product...

...colonies. Equipment Shaking incubator at 37 °C Stationary incubator at 37 °C Water bath at 42 °C Ice bucket...

...solution through a 0.22 μm filter to remove any precipitates. Stain each well with 1 mL of 0.1% crystal violet...

...Depending on the pipette you’re using, you will be rotating dials behind the numerical display or the top ...

...incubator. Section 3: BCD Feed and valproic acid supplementation During the 24–144 h post-transfection, supplement...