...to express either the optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the...

...intracellular Ca++ release with an optogenetic receptor, melanopsin, requiring brief exposure to 470nm blue light...

...short expression times, where as others, such as hSynapsin, are relatively weak and may require longer waiting...

...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by cholinergic neuron-targeting enhancer...

...optogenetics AAV collection, including channelrhodopsins, halorhodopsins, and more....optogenetics plasmids available in Addgene's collection. Opsin Excitatory Wild-type ChR2 ChR2/H134R soCoChR ChR2...Bidirectional BiPOLES Promoter CAG CaMKII CBA Dlx EF1a/nEF Synapsin E2 regulatory element Fluorophore GFP Red-wavelength...AAV9 AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2...EYFP Cre dependent PHPeB Gradinaru 107708 pAAV-hSynapsin- soCoChR-GFP Syn CoChR (soma-targeted) GFP Constitutive...Constitutive 9 Boyden 107712 pAAV-hSynapsin-FLEX-soCoChR-GFP Syn CoChR (soma-targeted) GFP Cre dependent...

...uses genetically encoded tools, such as microbial opsins, to control cellular activities using light. In...

...cations can affect the attachment of adherent cells) Trypsin EDTA (TrypLE, Thermo Fisher, 12605010) Ethanol,...

... 177471 Anti-Synapsin-3 [L125/121R] Synapsin-3 Human Mouse IgG2a 177472 Anti-Pan-Synapsin [L125/129R] ...Neuroligin-2 Mouse Mouse IgG2a 206608 Anti-Synapsin-3 [L125/76R] Synapsin-3 Human Mouse IgG2a 206610 Anti-Cav1.3...Mouse IgG2a 225366 Synapsin-3 (Homo sapiens) recombinant monoclonal antibody. Synapsin-3 Human Mouse IgG2a...Bassoon Rat Mouse 182079 pan-Synapsin scFv [L125/129] L125/129 pan-Synapsin Mouse 182080 Aldh1L1 scFv [...] Pan-Synapsin Mouse IgG2a 177473 Anti-SAPAP3 [L126/93R] SAPAP3 Mouse Mouse IgG2a 177474 Anti-GAD65/67...

...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...

... developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting...luciferin), it generates light that activates the opsin to either excite or inhibit the target neuron. For...

... CD147-spike protein . Cathepsin L - Bosch BJ, et al, 2008, J Virol. Cathepsin L Functionally Cleaves ... is involved in virus entry into cells. ( 4 ) Cathepsin L - a cysteine endosomal protease that triggers...

...additional fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies... Cre Yes Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock... Cre Yes Dual recombinase-dependent: Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock...

...tTA) 2 Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off transactivator...pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression of the...

...reporter VARNAM Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator. Neuron. 2023... mammalian neurons using engineered microbial rhodopsins. Nat Methods. 2014 Aug;11(8):825-33. Adam Cohen...D95N), MacQ-mCitrine, QuasAr and QuasAr-mOrange) Rhodopsin-based voltage imaging tools for use in muscles...

...1 CREB, MGC9284 CTSB cathepsin B APPS, CPSB CTSE cathepsin E CATE CTSL1 cathepsin L1 CATL, CTSL, FLJ31037...FLJ31037, MEP CTSS cathepsin S MGC3886 ERAP1 endoplasmic reticulum aminopeptidase 1 A-LAP, ALAP, APPILS, ...CHEMERINR, ChemR23, DEZ, MGC126105, MGC126106 CTSG cathepsin G CG, MGC23078 CX3CL1 chemokine (C-X3-C motif)...factor CCN2, HCS24, IGFBP8, MGC102839, NOV2 CTSG cathepsin G CG, MGC23078 CX3CL1 chemokine (C-X3-C motif)...

... Example: Jaws Browse Halorhodopsin plasmids . Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum...Microbial Opsin Variants Opsin Type Variant Description Peak Response Spectra (nm) Channelrhodopsins: cation...Optogenetics Plasmid Collection . Microbial Opsins Opsins are light-gated ion channels or pumps that absorb...trafficking signals to localize opsins to the cell membrane. Microbial opsins, such as those described below...commonly used microbial opsins, including a brief overview of the wild-type opsin of each and some examples...list of opsins here to find the right optogenetics plasmids for your experiments. Channelrhodopsins Channelrhodopsins... Browse Channelrhodospin plasmids . Halorhodopsins Halorhodopsins are light-gated inward chloride pumps...

...collection of PSAM plasmids ! LMOs Luminopsins (LMOs), or luminescent opsins, are opto-chemogenetic fusion ... enzyme generates light that activates the opsin. The opsin may excite or inhibit the neurons expressing... RE. (2015). Inhibitory luminopsins: genetically-encoded bioluminescent opsins for versatile, scalable...-fluorescent protein fusions, to more-sensitive opsins has led to improved LMOs with increased efficiencies...systems in our Optogenetics Guide . Figure 4: Luminopsins can be activated through chemogenetic or optogenetic...LMOs and their activity in neurons LMO Luciferase Opsin Ligand Effect Outcome (in neurons) Reference LMO3...Augustine GJ, Yin HH, Hochgeschwender U (2016). Luminopsins integrate opto- and chemogenetics by using physical...

... Ho 102364 pSIN-PAmCherry-mSNCA-NE SNCA NE EF-1 alpha Parkinson's Shu Leong Ho 102366 pSIN-hSNCA-NE SNCA...209085 piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623) SNCA TRE Parkinson's Vikram ...piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628) SNCA Avi TRE Parkinson's Vikram...

...hM4D(Gi) DREADD KOR DREADD PSAM4 GlyR Promoter Synapsin CaMKIIa CD68 Dlx GFAP nEF CAG E2 regulatory element...

...and OZ540 OPEN OPEN tTGCATCCACggcagTCGGCAGTGc Encephalopsin (Opn3) OZ541 and OZ542 OPEN OPEN aTCCATCCCAaacacatGTGGCAGAAt...

...CAG CaMKIIa CMV Dlx EF1a/nEF GFAP and variants Synapsin TBG Other Fluorophore/Tag Green Red None Other...

... CAG GCaMP8s Calcium sensor Looger 106174 pAAV.hSynapsin.SF-iGluSnFR.A184S Syn Tight affinity glutamate...

...useful (if not more so) for research applications, eclipsing past genome engineering technologies like zinc...

...adherent cells, aspirate media. Add 20 μl of 0.05% trypsin-EDTA to each well with a clone present. Resuspend...

...genes. The short coding sequences of channelrhodopsins, halorhodopsins, and other optogenetic genes enables...