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We narrowed to 1,100 results for: Ide

Showing: 81 - 120 of 1100 results

  1. Tetracycline Inducible Expression

    Type
    Collection
    ...cloning to control expression of your gene of interest. ID Plasmid Description Transactivator Promoter PI 21915... the transactivator for your tetracycline system. ID Plasmid Description Transactivator PI 26429 pLenti...search our collection for all dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible...Tetracycline Off Tetracycline On Experimental Considerations Tet Plasmids Inducible and repressible promoters...repressor protein (TetR) and operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA). In normal conditions...importantly, the TetR protein has been modified to provide two classes of tet-responsive systems: Tet-Off ...1995, Gossen et al. used random mutagenesis to identify the amino acid residues of TetR that were important...

  2. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP and puromycin... the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows for the simultaneous ... . Use these tools to help identify guide sequences that minimize identical genomic matches or near-matches...CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud to present a video reprint from the Journal...oligos for each guide and its associated reverse complement including additional nucleotides for cloning ...Add “CACC” before the 20-mer guide sequence and “AAAC” before the guide’s reverse complement for cloning...Journal of Visualized Experiments (JoVE). The video publication by Stuart Orkin's and Daniel Bauer's labs...

  3. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Cerebellum Whole brain Addgene ID Plasmid Allen Institute ID Enhancer ID FP/Recombinase Labeled Population...Caltech Systemic Capsids AAV Packaged on Request AAV Guide Plasmids Publications Enhancer AAV plasmids are ...

  4. Chemogenetics Plasmids

    Type
    Collection
    ... AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...Plasmid Collection You may also like... Chemogenetics Guide Viral Service: Chemogenetics AAV Blog: Chemogenetics... Actuator Modules (PSAMs). DREADDs are the most widely used chemogenetic receptors and are G-coupled protein... your experiments, please see our Chemogenetics Guide . Chemogenetics Plasmids Browse, sort, or search...

  5. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ... ) VPR ( Addgene #63798) Plasmids from Collection ID Plasmid Description PI Publications Kaufman, M. L...phosphorylated oligonucleotides containing the guide target sequence: Up to two guides can be expressed...utilizing one of the dual guide shuttle plasmids to insert a second guide within a U6-guide cassette. This process... shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2...desalted oligonucleotides with the appropriate overhangs: The plasmids listed here are provided as empty...Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual guide cloning: Brzezinski...in the context of the developing mouse retina. To aide in their studies of the regulatory networks involved...

  7. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI Due to ...

  8. Mammalian RNAi Tools

    Type
    Collection
    ...components, like argonaute, please search our full site . ID Plasmid Vector Type PI Publication Additional Resources...

  9. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...site", "active site loop", or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional...protein with many available isoforms and mutants provides a shared experience for the research community...

  10. AAV for Neuronal Tracing

    Type
    Collection
    ...virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...Rabies Virus-based Monosynaptic Tracing The core idea of the system is to infect the cell population of...deletion-mutant rabies, the essential G protein is provided to the starter cell in trans , via delivery by...

  11. Antibody Plasmid Collection

    Type
    Collection
    ...antibodies (search for "anti-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top ... encoding scFv fragments that bind to the GCN4 peptide, from the popular SunTag system, fused to sfGFP...antibodies and their applications in our Antibody Guide ! Cloning and Expressing Antibodies The following...

  12. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...synthases needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector...Bergendahl V, Marahiel MA.1998. Peptide bond formation in nonribosomal peptide biosynthesis. Catalytic role...MCP tagged plasmids for visualization of proteins inside cells. Depositor...ACP-, and MCP-tags for visualization of proteins inside living or fixed cells. The plasmids associated ...-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to SNAP-tag but reacts with benzylcytosine...are useful for labeling secreted proteins with disulfide bridges such as antibodies. MCP-tag is a mutant...

  13. Chemogenetics AAV Preps

    Type
    Collection
    ...AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement...See our Chemogenetics Plasmids and Chemogenetics Guide pages for more information about this field. Receptor... found to match all requested criteria. Please consider using fewer filtering options. *AAVrg = retrograde...

  14. AAV Packaged on Request

    Type
    Collection
    ... plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter PI Additional Resources...transduce many mammalian cell types. AAV is used in a wide range of experiments, both in vitro and in vivo....

  15. Brain Initiative Collection

    Type
    Collection
    ...publications associated with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes...for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity Recommended Applications...of the brain generated through this effort will provide novel ways to treat, cure, and even prevent brain... the BRAIN Initiative below. Support has been identified by depositors and/or when BRAIN Initiative grants...jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A. Mark Histed 179459-AAV1 pAAV-EF1a-DIO-JEDI...available at Addgene. AAV Viral Service . Addgene provides a viral service to help facilitate scientific ...

  16. Genetic Code Expansion

    Type
    Collection
    ...contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin ncAA Expression Codon PI...to enhance non-standard amino acid incorporation. ID Strain Description PI 48998 C321.ΔA all TAG sites...modified codons for unnatural amino acid incorporation. ID Plasmid Gene/Insert Vector Type PI...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian...genetic code for all life is based upon four nucleotides, 64 codons, and 20 amino acids. Yet in the past...from the tRNA is incorporated into the growing peptide chain. To expand the genetic code, modified tRNAs... the genetic code, there are several things to consider. If you are working off of a previously established...

  17. Validated gRNA Sequences

    Type
    Collection
    ...Species Target Sequence Plasmid ID Application Cas9 Species PubMed ID Depositor OCT4 H. sapiens CTCCCATGCATTCAAACTG...in the associated article (listed below by PubMed ID). These factors include: Does a given gRNA sequence...gRNA Target Sequences You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts...in Addgene's database as well as from sequences provided to us by users (see below for more details). Keep... Keep in mind that additional factors must be considered before using a validated gRNA sequence in your...

  18. Zebrafish Plasmid Collection

    Type
    Collection
    .... Large collections or kits are described below . ID Plasmid Description Gene/Insert PI Return to top ...rerio ), named for the horizontal stripes on the sides of the adult body, is a tropical freshwater fish...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology ...CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung...scientists in Latin America. The Zebrafish Book - A guide for the laboratory use of zebrafish. CRISPRz - A...zebrafish-specific gene expression database that provides information about when genes are active in developing...

  19. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...click on the plasmid name for ordering information. ID Name Promoter Description Category PI 157970 pTR-...application TMN200 (200 mM sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable for...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...retina following subretinal injection. It was identified by John Chiorini and characterized by Shannon...

  20. Cre-lox system

    Type
    Collection
    ...of plasmids that are currently in our repository. ID Plasmid Description Promoter Expression System PI...Empty backbones to regulate your gene of interest: ID Plasmid Description Expression System PI 11578 pSico...selecting desired clones, and making transgenic mice. ID Plasmid/Article Description Expression System PI ... Cre; NeoR act15 D. discoideum Kay 108996 pDM1489 Cre; HygroR act15 D. discoideum Kay 109378 LZF40: LZF40...Cre expression once it is induced. Addgene has a wide variety of Cre recombinase plasmids. Special features...Cre expression plasmids. Addgene's viral service provides high-quality and high-titer viral preparations...65727 pcDNA3.1-CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular vesicle targeting sequence and ...

  21. TALEN Plasmids and Kits

    Type
    Collection
    ...Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description Golden Gate TALEN...You may also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs are important...repeats in the TAL effectors. View Addgene's TALEN Guide. These collections and accompanying documentation...

  22. Control AAV Preps

    Type
    Collection
    ...VCre-dependent Constitutive (non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI...We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection... found to match all requested criteria. Please consider using fewer filtering options. Browse all Control...

  23. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Lab Improved Phosphoprotein Synthesis Reagents Type ID Item Strain 192872 rEcoli XpS (MG1655-C321 mutS+,... Human Serine Phosphopeptide Library described in: Encoding human serine phosphopeptides in bacteria for... for proteome-wide identification of phosphorylation-dependent interactions. Barber KW, Muir P, Szeligowski...Substrate Profiling Using a Proteome-wide Serine-Oriented Human Peptide Library. Barber KW, Miller CJ, Jun...serine phosphorylation ( Rinehart Human Serine Phosphopeptide Library ). The phosphorylation sites (“phosphosites...phosphoserine site flanked by 15 amino acids on either side occurring within the parent protein. Using the recoded...purifying the phosphosite library as GST-fusion peptides (“mode #1” expression). The mode #1 phosphosite...

  24. Rett Syndrome

    Type
    Collection
    ...bacterial) Insert species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return...toddlerhood primarily affecting females at an incidence of approximately 1:10,000. It is characterized...for proper nervous system function. While there is wide variability in symptoms even within patients with...window) PMID: 1606614 1999 - Huda Zoghbi's lab identifies MECP2 as the genetic cause of RTT. (Link opens...the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology ... Rett syndrome in Australia: a review of the epidemiology. J Pediatric . 148(3):347-352. (Link opens in...

  25. Optogenetics AAV Preps

    Type
    Collection
    ...AAV1 AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV... See our Optogenetics Plasmids and Optogenetics Guide page for more information about this field. Use ... found to match all requested criteria. Please consider using fewer filtering options. Browse all Optogenetics...

  26. Retrograde AAV viral preps

    Type
    Collection
    ...Activators Inhibitors Other Molecular Tools Clear Filters ID Name Promoter Activity Category PI 37825 AAV-CAG-... found to match all requested criteria. Please consider using fewer filtering options. Browse all Retrograde...

  27. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Library Size Genome-wide Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type Species...4096 (4 6 ) combinations of 6 nucleotides. Chong Zhang E. coli Genome-wide Inhibition Library 113134 — ...Human genome-wide library v1 69763 Knockout Human Wu 3rd 4 77,406 Human improved genome-wide library v1 ...genome-wide library v1 Discontinued Knockout Mouse Yusa 3rd 5 87,897 Mouse improved genome-wide library...Perturb-seq Guide Barcodes (GBC) 85968 Barcode Human Weissman 3rd N/A N/A Retroviral Mouse Genome-wide CRISPR...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...Adamson 3rd ∼3 366 or 1,573 Activity-optimized genome-wide library Discontinued Knockout Human Sabatini and...

  28. Biosensor AAV Preps

    Type
    Collection
    ...AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor Label Activity Serotype PI Acetylcholine... found to match all requested criteria. Please consider using fewer filtering options. *AAVrg = retrograde...

  29. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target Species Host Species Isotype...produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma Target Target Species Host Species...nanobodies from transfected bacterial cells. Addgene ID Plasmid Hybridoma Target Target Species Host Species...IgG2a 188173 Anti-NPY2R/Neuropeptide Y receptor type 2 [L119/134R] NPY2R/Neuropeptide Y receptor type 2 Human...Parvalbumin Rat Mouse IgG2b 206639 Anti-Neuropeptide Y [L115/13R-2b] Neuropeptide Y Human Mouse IgG2b 206640 Anti-VGAT... 211594 Anti-NPY2R/Neuropeptide Y receptor type 2 [L119/106R-2b] NPY2R/Neuropeptide Y receptor type 2 ...Rat Mouse 190505 NPY/Neuropeptide Y scFv [L115/13] L115/13 scFv 2t NPY/Neuropeptide Y Human Mouse 190506...

  30. Immunology Research Plasmids and Resources

    Type
    Collection
    ...CCN3, IGFBP9 NPFF neuropeptide FF-amide peptide precursor FMRFAL NPPA natriuretic peptide precursor A ANF... PIK3CD phosphoinositide-3-kinase, catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase...natriuretic peptide precursor B BNP NPPC natriuretic peptide precursor C CNP NPR1 natriuretic peptide receptor... HBGF8, HBNF, NEGF1 PYY peptide YY PYY1 QRFP pyroglutamylated RFamide peptide 26RFa, MGC119794, P518 RABEP1...class I polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related...

  31. Plan Your Experiment

    Type
    Collection
    ... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan Your Experiment...selection marker to identify and enrich positive cells Packaging and envelope plasmids provide the necessary...see our Lentivirus Guide ) Stable, tunable expression of Cas9 and/or gRNA in a wide variety of mammalian... desired edit, ideally less than 10 bp away. In this case, it is necessary to identify the exact location... increase specificity, you can also consider using a high-fidelity Cas enzyme. In addition to off-target...the genome like never before. This section will provide a general framework to get you started using CRISPR...apply to using CRISPR in other organisms. First, consider the genetic manipulation that is necessary to ...

  32. Zhang Lab CRISPR Page

    Type
    Collection
    ...along with the guide RNA. For most applications, we recommend using Cas9 with the single guide RNA (sgRNA)...that adding an additional guanine nucleotide to the 5’ of 20-bp guide can increase targeting efficiency...PX330; SpCas9 and single guide RNA 48138 : PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9...2A-Puro and single guide RNA 48873 : PX460; SpCas9n (D10A nickase) and single guide RNA 48140 : PX461;...nickase) and single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165...below. Pooled genome-wide human SAM library SAM can be combined with a human genome-wide library to activate...that adding an additional guanine nucleotide to the 5’ of 21-bp guide can increase targeting efficiency...

  33. CRISPR References and Information

    Type
    Collection
    ...Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide to using CRISPR plasmids in your lab, from...Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK). This tool can identify positively or negatively...location, pasted nucleotide sequence, or a sequence text file you upload. Currently supports a wide selection...screens targeting a long or short list of genes provided by the user. Developed by the Fredrik Wermeling... 2.0 (Link opens in a new window) A tool that identifies putative target sites for S. pyogenes Cas9, S...Institute. Cas-OFFinder (Link opens in a new window) Identifies gRNA target sequences from an input sequence ...online predictor (Link opens in a new window) Identifies candidate sgRNA target sites by off-target quality...

  34. Viral Vectors

    Type
    Collection
    ...NIH provides basic guidelines for working with various types of viruses; however, these guidelines may...engineered to harbor a wide array of viral genomes, enabling researchers to direct a wide array of genetic ...viruses for a wide array of research purposes. Which Type of Virus Should I Use? This table provides a brief...Lentivector Toolbox Guide to Generating Recombinant Adenoviruses Eric Campeau's Viral Vector Guide University ...general virus biosafety guidelines, check out Addgene's Biosafety Resource Guide . References Cohen S, ... Also Like... Addgene's Viral Service Biosafety Guide Blog: Plasmids 101-Viral Vector Elements Viral Vectors...Plasmids Which Type of Virus Should I Use? Use this guide to learn more about the different types of viruses...

  35. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...display less off-target activity. High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9...Joung JK. 2016. High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. ... Genome Engineering You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts...crRNAs). Through sequence homology, these crRNAs guide a Cas nuclease to the specified exogenous genetic...but it is not invincible. Bondy-Denomy et al. identified the first set of anti-CRISPR genes in bacteriophages... screens. Pooled gRNA libraries can be used to identify genes that are important to a given phenotype....sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR ...

  36. Ras Pathway

    Type
    Collection
    ...Ral guanine nucleotide dissociation stimulator RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide exchange factor...GTPase and is a member of the G protein (guanine nucleotide binding protein) family. GTPases serve as molecular...isoforms, individual links to each gene page are provided below. Color is used for clarity and does not ...isoforms, individual links to each gene page are provided below. Symbol Name AKT AKT1 AKT2 AKT3 v-akt murine...activating protein 35 ARHGEF2 Rho/Rac guanine nucleotide exchange factor 2 CCND CCND1 CCND2 CCND3 Cyclin...factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth factor receptor EIF4EBP EIF4EBP1 EIF4EBP2...2 like 2 NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 PAK PAK1 PAK2 PAK3...

  37. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited...and denoted as such. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...Lentiviral BbsI none S. pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI none S....Mammalian BbsI yes, cut, C-terminal S. pyogenes Zhang pGuide 64711 Mammalian BbsI none S. pyogenes Musunuru ...Mammalian/Lentiviral H1-Tet none S. pyogenes Herold lentiGuide-Crimson 70683 Mammalian/Lentiviral hU6 none S...

  38. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...CRISPR guides. The first one binds to sfgfp with either 0, 10, 11, 14, or 20 matching nucleotides, the ...Repression Plasmids You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts...decreasing the expression level. Mismatches between the guide RNA and the target sequence lower the repression...mcherry with either 0, 11, 18, or 20 matching nucleotides. Together, these plasmids allow to explore the...controlled using a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with CRISPR repeat motifs...

  39. Antibody Production

    Type
    Collection
    ...phosphate-buffered saline containing 1 mM sodium azide. This buffer is not compatible for use in live cells...sample is separated via a denaturing SDS-PAGE gel alongside serial dilutions of a standard antibody of known... undergoes testing, either in-house or through outside labs, to confirm recognition of the expected target...parental plasmid sequence to confirm the antibody’s identity. For more details on protocols see the Addgene...at room temperature. However, Addgene determines ideal shipping conditions for each order based on the ...you choose, store, or use your antibody. Antibody Guide Antibody Protocols Recipient Instructions Antibody...

  40. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...backbone to use, you have many elements to consider. Here is a guide to a selection of Addgene's empty vector...such as Flag or HA, will allow you to easily identify your protein using an antibody against that epitope... our full Fluorescent Protein (FP) Guide and the Michael Davidson FP Collection NLS Nuclear...N-terminal His and NusA fusions Return to top Function Besides functions such as protein purification mentioned... plasmid in Pseudomonas species Vectors for RNA-guided genome editing in plants Visit our CRISPR Plasmids...Protein-protein interaction Proximity-dependent biotin identification (BioID) MCS-BioID2-HA or myc-BioID2-MCS - To...
Showing: 81 - 120 of 1100 results