...Collection for plasmids that can be used to create cell lines with endogenously-tagged gene variants or browse...human cells to pluripotency by activating enogenous genes Human pluripotent reprogramming with CRISPR activators...lentiviral plasmids Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 ...stem cells. Stem Cells. 2009 May . 27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral...from four separate plasmids Defining molecular cornerstones during fibroblast to iPS cell reprogramming ...iPSCs Bipolar Neurons Lentiviral Human Rapid neurogenesis through transcriptional activation in human ...

... induced pluripotent stem cell (hiPSC) lines. These cell lines were created using CRISPR and the donor...tagging transcription factors with FLAG in human cell lines. To repeat the tagging in your system, use the ...terminal affinity tag (3xFLAG-2xSTREP) on endogenous genes for the isolation of native protein complexes. This...be scaled up for systematic editing of multiple genes. Plasmids can be found associated with the following...

...Empty Backbones mEmeralds Reds Blues Cyans Oranges Greens Yellows Photoactivatables Empty Backbones Plasmid...lab fluorescent protein fusion constructs and backbones. Plasmid...Collection More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics The...

...more effective than dCas9 alone for mammalian cell lines. Activate (CRISPRa) Increase expression of an endogenous...cells, or selection marker to generate stable cell lines (for details, see Plasmids 101: Mammalian Vectors...and/or gRNA in a wide variety of mammalian cell lines Useful for difficult to transfect cell types, can...Protocol for genomic deletions in mammalian cell lines Select Your Target Sequence and Design Your gRNA...subcloning into a plasmid, and screening individual clones. PCR amplification and next-generation sequencing...

...modifications to the the pX458 plasmid to expand its usefulness for the lab's gene regulation and developmental...The plasmids listed here are provided as empty backbones that utilize Golden Gate cloning to insert the...E., Schneider, S. R., Abraham, J., Hensley, A., Jones, K. L., & Brzezinski, J. A. (2021). Initiation of...fate specification, yet differs from deleting both genes . Development. doi: 10.1242/dev.190272. PMID: 32541005...

...of wild-type malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate...diverse set of wild-type MDH genes from several species and mutant clones from watermelon have been codon-optimized...

...targeted to various zebrafish genes. The table below lists zebrafish genes and associated pairs of zinc...A list of zebrafish genes and associated pairs of zinc finger arrays that can be used to generate ZFNs...):1471-1501. PubMed PMID 19798082 . Targeted mutagenesis in zebrafish using customized zinc-finger nucleases...

...GTPase Plasmids by Gene The following table lists genes in the ARF GTPase family, information on each gene...list of plasmids available. Please note that some genes may not yet have plasmids available for request.... GEF Plasmids by Gene The following table lists genes in the ARF GAP and GEF family, information on each...

...in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name arose from its...pro-apoptotic effects is especially important to tumorigenesis. Inheriting only one functional copy of the ... 1; also known as GTSE1 BAI-1 Brain-specific angiogenesis inhibitor 1 Bax BCL2-associated X protein Bid... phenotypes. Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann H, Lu X, Soron G, Cooper...

... effectors, scientists in several biological disciplines are coming closer to this DNA targeting ideal...di-residue (RVD). Ultimately, it is this RVD that determines which single nucleotide the TAL effector will... Daniel Voytas’s group from the University of Minnesota, arrays can be assembled in a matter of days. ...can be assembled and inserted into a variety of backbones in just a few steps ( Figure 2 ). The Bogdanove...

... Depositor Collections FreeGenes Project Open Enzyme Collection FreeGenes Project Open Enzyme Collection...Open Enzyme Collection consists of more than 75 genes for enzymes that are considered "workhorses" of ...Additionally, the Open Enzyme collection contains enzyme genes for fixing damaged and degraded DNA prior to amplification...

...10.1186/s13104-018-3262-4. PubMed . Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis...Tanigawa N, Yuno M, Ikawa M, Taniuchi I, Fujii H. Genes Cells. 2018 Apr;23(4):318-325. doi: 10.1111/gtc.12573... Fujita T, Yuno M, Suzuki Y, Sugano S, Fujii H. Genes Cells. 2017 Jun;22(6):506-520. doi: 10.1111/gtc.12492... ribonucleoproteins. Fujita T, Yuno M, Fujii H. Genes Cells. 2016 Apr;21(4):370-7. doi: 10.1111/gtc.12341...

...single nucleotide variant lines in an isogenic cellular background. All cell lines and phenotypic datasets...The following plasmids can be used to create cell lines with endogenously-tagged gene variants or to differentiate...

...Michael Ruppert Michel Sadelain James Thomson Tim Townes Robert Weinberg Knut Woltjen Shinya Yamanaka Feng... metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is ...Addgene or email [email protected] to get started. Genes KLF gene pages with information on available plasmids...

...in between a protein and a tag, such as an NLS or NES, enables control over localization. Inserting PhoCl...acrIIA1 Listeria monocytogenes  pRAU168  No  pJH372  No  acrIIA2 Listeria monocytogenes  pRAU171 Partially...  Listeria monocytogenes  pCSW65 N/A (Toxic)   None N/A   acrIIA4 Listeria monocytogenes  pCSW21  Yes...quickly and easily generate zebrafish transgenic lines to study neurobiology and neuorodgenerative diseases...compatible cloning sites for easy cloning of your genes of interest. In order to improve the study of specific...induction of structural plasticity in single dendritic spines. The set of CyRFP constructs available at Addgene... used a combination of deliberate and random mutagenesis to isolate the best RFP. They targeted known ...

... endogenous human genes Guide RNA expression plasmids for endogenous zebrafish genes...these vectors for efficiently modifying endogenous genes in zebrafish (Hwang and Fu et al., Nat Biotechnol...

...Protein Plasmids & Resources You May Also Like... Luminescence: Luciferase Plasmids Blog: Which FP Should I...Fluorescent Protein Plasmids by Category Empty Backbones Understand the localization and/or function of...fluorescent proteins, as well as over 100 empty backbones. Fluorescent Protein Kits cpFRET Kit: FRET based...

...(toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium (cryptosporidiosis), Leishmania...have enabled precise genetic analyses to pinpoint genes linked to parasite virulence, transmission, and ...10 guides against 8,158 predicted protein-coding genes. Do you have suggestions for other plasmids that...

...simultaneously. By fusing sfGFP and/or mCherry to genes of interest, it is thus possible to study the effect...enables robust, noiseless knockdown of bacterial genes. Molecular Systems Biology, 14(3):e7899. doi: 10.15252... CRISPR knockdown to modulate expression of two genes without cross‐talk. (A) Schematic of the strain ...

...AAVs can be handled at BSL-1, AAVs expressing oncogenes or toxins should be handled at BSL-2. AAV can ...plasmids (e.g., pHelper) and specific packaging cell lines (e.g., HEK293 cells) during AAV production. Read...encoding AAV Rep and respective capsid protein which determines the AAV serotype) are used in conjunction with...

...a variety of pathways, which ultimately turn on genes involved in cell growth, differentiation and survival... related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral...National Cancer Institute (NCI) References Ras oncogenes and their downstream targets. Rajalingam K, Schreck...

...PI FusX TALEN Assembly System Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD ...mammalian systems including hard-to-transfect cell lines. Features include (i) Esp3I (BsmBI) restriction ... has successfully been employed for deletion mutagenesis and gene targeting in human and mouse pluripotent...

...screened thousands of enhancers for specificity and brightness of fluorescent labeling across brain regions ... top vectors with the highest specificity and brightness, assessed using ssRNAseq or high-resolution imaging.... A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types. bioRxiv...

...Regulation More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Biosensors Optogenetics...modular components or take advantage of existing GFP-lines for cell-specific manipulation. Plasmids Fluorescent...

...Arrays for Zebrafish Genes - Browse pre-constructed zinc fingers targeting specific genes Oligomerized Pool...

...developing novel diagnostics, therapeutics, and vaccines against COVID-19 all rely on DNA encoding viral...COVID-19 and Coronavirus Plasmids & Resources page outlines our entire COVID-19 collection. Additionally, ...

...contains 1,836 genes encoded by 3,548 isoforms and two control vectors. The individual clones, or the pooled...

... Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics...Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion Immunology Mammalian RNAi...

... lists gRNA plasmids designed to target various genes or genomic regions. It is highly recommended that... effect. Which species or variant of Cas9 ( S. pyogenes , S. aureus etc.) was this gRNA sequence designed...

...the germline. Using an injection approach, stable lines with targeted genome alterations can be generated... donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site...

...TALENs for Endogenous Zebrafish Genes TALENs for Endogenous Human Genes TALENs for the EGFP Reporter Gene...

...to investigate the roles of specific genes or to knockdown genes with potentially harmful mutations in...

...is designed to target promoters or enhancers of genes of interest. Different flavors of activators were...described in: Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator...

... a ligand binding domain, which interacts with hormones or other molecules, and 2) a DNA binding domain...homeostasis, and the receptors and their downstream genes are associated with numerous disease pathways. In...

...'s genome, which can lead to oncogenesis (termed insertional mutagenesis). Read our γ-Retrovirus Guide...

...organisms, or use our Search page to look for specific genes or applications that are not listed here. Addgene...Haemophilus influenzae Helicobacter pylori Listeria monocytogenes Mycobacterium sp. Neisseria sp. Pseudomonas ...

...response. Most requests will be approved within two business days! Ordering At your convenience After approval...can begin processing your order. MTA Approval 1+ business days, varies by requesting organization Viral ...

...expressing both the Cre and Flp recombinases to remove genes flanked by either loxP or frt sites respectively...reporters as well as the Cre and Flp recombinases. Genes can be cloned into these transfer vectors using ...

...Cre dependent 9 Sanes 45186 AAV-EF1a-BbChT EF1a mCherry and mTFP Cre dependent 9 Sanes 114471 pAAV-Ef1a-fDIO...

...application TMN200 (200 mM sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable for in...model of complete congenital stationary night blindness. Hum Mol Genet . Nov 1;24(21):6229-39. PMID: 26310623...