...phenomenon to the presence of terminal proteins capping the ends of linear double-stranded AdV genomes....vectors (Lukashev et al., 2016). The protein capsid encapsulating the genome is majorly composed of three ...large transgene packaging capacity. For instance, with a packaging capacity of about 6 kb, the 1st generation...AdVs have already a packaging capacity larger than AAV vectors. This capacity is large enough to carry the...

...plasmid, a plasmid encoding rep and serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences...intensity of the viral capsid proteins are analyzed. The abundance of viral capsid proteins as a fraction...the relative stoichiometric ratios of the viral capsid proteins VP1, VP2, and VP3. Samples of viral preparations...non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are shown relative to...

...produce AAV: 1) the RepCap plasmid which provides the AAV replication (rep) and capsid (cap) genes. AAV replication...that's then packaged into AAV’s protein shell, or capsid; 2) the pHelper plasmid which expresses adenovirus...

...Sequence (SITS). This sequence bypasses the 5′ mRNA cap required for eukaryotic systems and instead directly...

...culture to air and make sure to tighten the flask cap before placing them in the incubators. This will ...ensure complete sterilization as mycoplasma can even escape 0.2-micron filters. Figure 1: Contamination...easily visible on the agar plates. This method is capable of detecting most of the mycoplasma species, apart...be overcrowded (filled no more than 60% of its capacity) which can cause mycoplasma infection from one...

...database, which relies on CAGE-seq to directly capture the mRNA cap, provides the most accurate mapping (Radzisheuskaya...thus frameshifts that disrupt the protein-coding capacity of a locus. When using S. pyogenes Cas9 (SpCas9...gRNAs is important to consider. While the basic landscape of mismatches that can nevertheless still lead...

...Christopher D. Lima. "The polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases...with products identifiable and quantifiable by capillary electrophoresis (CE) (see below). This method ...novel 16S rRNA gene PCR-ligase detection reaction-capillary electrophoresis assay." Journal of clinical microbiology.../LDR/flow-through hybridization assay using a capillary tube, probe DNA-immobilized magnetic beads and...PMC1978145. 27. Greenough, Lucia, et al. "Adapting capillary gel electrophoresis as a sensitive, high-throughput...

...begins, by definition, at the beginning of R, is capped, and proceeds through U5 and the rest of the provirus...retroviral particle containing Matrix, Capsid, and Nucleocapsid components. Pol in trans Precursor protein... (Ψ) in cis RNA target site for packaging by Nucleocapsid. WPRE in cis Woodchuck hepatitis virus post‐... of a retroviral provirus (see below). Cloning capacity between the LTRs is ∼8.5kb, but inserts bigger...

...begins, by definition, at the beginning of R, is capped, and proceeds through U5 and the rest of the provirus...lentiviral particle containing Matrix, Capsid, and Nucleocapsid components. Pol in trans Precursor protein...retroviruses (γ-retroviruses) is that lentiviruses are capable of infecting non-dividing and actively dividing... (Ψ) in cis RNA target site for packaging by Nucleocapsid. RRE in cis Rev Response Element; sequence to... of a retroviral provirus (see below). Cloning capacity between the LTRs is ∼8.5kb, but inserts bigger...

...antibodies are capable of recognizing and binding to epitopes on antigens, they are also capable of recognizing...In a sandwich ELISA, a capture antibody, bound to a surface, is used to capture the protein of interest...also be included. Antibody Applications - Capture Methods Capture methods such as immunoprecipitation (IP...variation of IP uses antibodies to capture protein:DNA complexes. After capture, the DNA is released from the...fragmentation of the DNA. Controls for capture methods Capture methods should use a positive control and...methods. Capture MS validation This method uses peptide identification by proteomics (Capture MS) to validate...Amplification Application Overview Quantification Methods Capture Methods Visualization Methods Cell Sorting Methods...

...iCre CAG Mammalian Capecchi 113849 pCAGGS-pac-T2A-iCre iCre CAG Mammalian Capecchi 116879 CAG-Cremyc-2A-GFP...Mammalian Capecchi 125749 pCAG-sfGFP-iCre-ERT2 iCre-ERT2 - Tamoxifen inducible CAG Mammalian Capecchi 125821...Mammalian Capecchi 125822 pCAGGS-mTagBFP2-T2A-iCre (v2) TagBFP, iCre CAG Mammalian Capecchi 126006 pAAV2...domain CMV Mammalian Green 12496 p270 pCMV-CRE-M-AR(LNCaP) CREM fused to mutant AR ligand binding domain CMV...unskippable linker) - Tamoxifen inducible CAG Mammalian Capecchi 128167 pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2...GSAx9 linker) - Tamoxifen inducible CAG Mammalian Capecchi 128168 pCAGGS-mTagBFP2-T2A-iCre-ERT2 iCre-ERT2...-terminus) - Tamoxifen inducible CAG Mammalian Capecchi 128171 pCAGGS-mTagBFP2-T2A-iCreERT2 (PV) iCre-...

..., and dogs. This serotype was derived from AAV2 capsid and developed by Arun Srivastava’s lab. AAV2(trpYF...Arun Srivastava’s lab. It is derived from the AAV2 capsid and contains the following mutations: Y444F, Y500F...Arun Srivastava’s lab. It is derived from the AAV6 capsid and contains the following mutations: Y705F, Y731F...19066593 Other citations include: Bogner, et al. 2015. Capsid Mutated Adeno-Associated Virus Delivered to the...Kanaan, et al. 2017. Rationally Engineered AAV Capsids Improve Transduction and Volumetric Spread in the...al. 2016. Microglia-specific targeting by novel capsid-modified AAV6 vectors. Mol Ther Methods Clin Dev...

...targeting the SARS-CoV-2 nucleocapsid protein (Brian Geiss). Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] - ...monoclonal antibodies that target SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that...COVID-19 research. Antibodies Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse...monoclonal antibody targeting the SARS-CoV-2 nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids...RBD mutants that can be used to identify antibody escape mutants and for comprehensive mapping. Some of ...

... others work to increase Cas9’s proofreading capabilities. No matter the method, increased fidelity enzymes...by the Feng Zhang lab ) combine the large-scale capacity of transposases with the precise DNA-targeting...programmability for different genomic loci, and the capability to detect multiple targets simultaneously. CRISPR...and biotin tags, or an anti-Cas9 antibody. The CAPTURE system, developed by the Jian Xu lab , utilizes... Cas12a also has more condensed multiplexing capabilities , as it can process multiple gRNAs under a single...carrying an ADAR truncation that retain RNA editing capabilities but are small enough to be packaged in AAV particles...two nuclease domains inactivated. This enzyme is capable of cleaving only one strand of target dsDNA. Off-target...

...they are capable of differentiating into every other cell type in the body and have the capacity to give...are proliferative, unspecialized cells with the capacity to differentiate into many different cell types... Acad Sci U S A. 2014 Jul 22;111(29):10678-83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged...

...sometimes require preps beyond our current in-stock capabilities. To meet that need, our Packaged on Request ...globally connected scientific community. The Data Hub captures valuable knowledge that often goes unshared, bridging...troubleshooting and results that complement published data, capturing the insights that often go unshared. Help Center...

...Mammalian (pEGFP-C2) GAP Acap1 9744 740 Mammalian (pFLAG-CMV2) GAP Acap2 23527 778 GAP Acap3 116983 834 GAP Agap1...

...nickase fused to a reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and... to immunoprecipitate FLAG-tagged Cas9. In the CAPTURE system, Cas9 is tagged with a biotin acceptor site...streptavidin affinity purification. For both enCHIP and CAPTURE, design your gRNA sequence to direct dCas9 to a...

...polypeptide 1 (pituitary) receptor type I PAC1, PACAPR, PACAPRI ADIPOQ adiponectin, C1Q and collagen domain...receptor 1 FLJ41949, HVR1, II, PACAP-R-2, RDC1, VAPC1, VIPR, VIRG, VPAC1, VPCAP1R VIPR2 vasoactive intestinal...C5R1, CD88 CAMP cathelicidin antimicrobial peptide CAP18, CRAMP, FALL-39, FALL39, HSD26, LL37 CCBP2 chemokine...MGC138386, NDI, V2R AZU1 azurocidin 1 AZAMP, AZU, CAP37, HBP, HUMAZUR, NAZC BDNF brain-derived neurotrophic...CGRPR, CRLR CAMP cathelicidin antimicrobial peptide CAP18, CRAMP, FALL-39, FALL39, HSD26, LL37 CAT catalase...intestinal peptide receptor 2 FLJ16511, VPAC2, VPCAP2R XCL1 chemokine (C motif) ligand 1 ATAC, LPTN, LTN, SCM...

...adenoviruses in research include their large packaging capacity (>8 kb), high titers, and high levels of transgene...substantial immune response induced by the viral capsid. Adenoviruses can be produced by several methods...