...speak of excitation and emission spectra.
Both spectra possess clear maxima where excitation and emission...laser excites at a wavelength of 488 nm (Table 1), which is near a local maximum of PE's excitation spectrum...laser, however, excites at 561 nm, which is close to the global maximum of PE's excitation spectrum where...signal.
Figure 2: Excitation and emission spectra for PE. The excitation spectrum shows two maxima...the red laser excites at 640 nm (Table 1), while comparing the two fluorophore's excitation spectra around...that APC-Cy7 is excited at a lower efficiency than APC. While APC's global excitation maximum lies around...-Cy7's global excitation maximum is about 750 nm. If we were to use a laser that excites at 750 nm, APC-Cy7...