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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 932 results for: Kin

Showing: 41 - 60 of 932 results

  1. p53 Pathway

    Type
    Collection
    ...CDK6 Cyclin-dependent kinase 4 or 6 CHK1 Checkpoint kinase 1 CHK2 Checkpoint kinase 2 Cop-1 Ring finger ...factor 1 ATM ATM serine/threonine kinase ATR ATR serine/threonine kinase B99 G-2 and S-phase expressed 1...PMAIP1 p14ARF Cyclin-dependent kinase inhibitor 2A p21 Cyclin-dependent kinase inhibitor 1A p48 Damage-specific...

  2. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Experiments (JoVE). The video publication by Stuart Orkin's and Daniel Bauer's labs details the use of CRISPR...Cell Lines via CRISPR/Cas9. Bauer DE, Canver MC, Orkin SH. J. Vis. Exp. (95), e52118, doi:10.3791/52118...deletion). Use a pair of forward and reverse primers flanking each sgRNA target site (within 150 - 350 bp) to...Buffer, 6.5 μl ddH 2 O, and 0.5 μl T4 Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated oligos...This is a representative sequencing primer; other flanking primers may be utilized. Choose a sequence-verified...at 400 x g for 5 min and remove supernatant by flicking the PCR plate over a sink. Add 50 μl of DNA extraction...

  3. COVID-19 Resources

    Type
    Collection
    ... ) PCP4 - (Purkinje cell protein 4) is involved in beating of airway cilia.( 7 ) Cytokines - SARS-CoV-...infection induces a cytokine storm response in the body. For a full list of cytokines and related plasmids...family called SARS-CoV-2. Scientists have been working at lightning speed to elucidate as much as they...and Yeast expression Spike protein constructs for making VSV pseudo virus Mammalian constructs Due to the... Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad Institute...

  4. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...pegRNA + nicking sgRNA BbsI No Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA...promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and ...mRFP1 David Liu 140448 QPM-sgR (pTaU3) Plant TaU3 nicking sgRNA Eps3I + NcoI No Caixia Gao 141081 pYPQ141D-peg...Weber 172717 pnsgRNA Bacteria J23119 (BBa_J23119) nicking sgRNA No Tilmann Weber 173220 pPBT-peRNA_GG-Puro...sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum Kim 177180 pDAS12069_...

  5. CRISPR Guide

    Type
    Collection
    ...dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and RNA tracking in living... the most scalable genome editing technologies, making CRISPR perfect for genome-wide screens. This guide...very close proximity to the region to be edited, making the PAM sequence (NGG) limiting. This PAM is abundant...inhibit CRISPR by a variety of mechanisms, including blocking crRNA loading or PAM recognition; inhibiting Cas9...sites is sufficient to repress transcription by blocking initiation. More robust transcriptional repression...repression through cytosine methylation with improved kinetics LSD1 - repression through targeted removal of ...proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal RNA...

  6. MAPK Plasmids

    Type
    Collection
    ...Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated protein kinases (MAPKs...extracellular signal-regulated kinases (ERKs), are serine/threonine-specific protein kinases. They regulate diverse...Browse mitogen-activated protein kinase (MAPK) plasmids. Plasmid...

  7. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... of kinases of interest by mass spectrometry in a technique called serine-oriented library/kinase-library...111705-8 ) is available from Addgene. Screening kinases. The mode #1 phosphosite library can also be synthesized...7):638-644. PubMed (Link opens in a new window) Kinase Substrate Profiling Using a Proteome-wide Serine-Oriented.... PubMed (Link opens in a new window) The PINK1-PARKIN Mitochondrial Ubiquitylation Pathway Drives a Program...

  8. Lentivirus Plasmids

    Type
    Collection
    ...are some safety considerations to evaluate when working with lentivirus: these viruses are based on HIV...components Trono 63586 psPAX2-D64V 2nd Packaging For making Integrase deficient lentivirus Rawlings , Scharenberg...2nd and 3rd generation lentivirus technologies. Looking for lentiviral plasmids to express your CRISPR ... ORF expression, multicistronic design enables tracking of viral transduction. Also see plasmid 46948 ...inducible knockdown, multicistronic design enables tracking of viral transduction. See article for more similar...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...brighter fluorescence and faster kinetics (CaMPARI2) Improved methods for marking active neuron populations....fluorescent calcium sensors (fRCaMP1/2, GECO1/2) The kinetic mechanisms of fast-decay red-fluorescent genetically... novel analytical method for in vivo phosphate tracking. FEBS Lett. 2006 Oct 30. 580(25):5885-93. Wolf... Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission...Glutamate iGluSnFR glutamate sensor with improved trafficking to the plasma membrane Acute disruption of the...expression of pH sensitive mt-mKeima) The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy.... Vladislav Verkhusha Return to Top Browse All Looking for a sensor for a different analyte, a sensor ...

  10. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ... Collections MJFF Parkinson's Plasmid Resource Michael J. Fox Foundation Parkinson's Disease Plasmid Resource...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation... researchers to assemble and share tools for Parkinson's Disease research. This collection is part of ...

  11. Zhang Lab CRISPR Page

    Type
    Collection
    ...Cre-dependent and constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice ...applications. Full references are below. The Cas9 knockin mouse can be purchased from Jackson Labs: Cre-dependent... for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling 60224 : sgRNAs...10.1038/nbt.2647. Epub 2013 Jul 21. PubMed . Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing...plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice for genome editing and cancer modeling. Platt...

  12. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Human Doench 3rd 1, 2, or 4 49,766 arrays Broad GPP kinome Brunello 75314, 75315 (1 plasmid) 75312, 75313 ...Knockout Human Doench and Root 3rd 4 3,052 Broad GPP kinome Brie 75317 (1 plasmid) 75316 (2 plasmid) Knockout...(Set A) 67,194 (Set B) Cas13a/C2c2 Protospacer flanking site (PFS) Library 79153 Knockout E. coli Zhang... Human Weissman 3rd 6 Varies Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 ...Library 140633 Knockout Human Wei 3rd 3 58,630 Human Kinase Domain-Focused CRISPR Knockout Library 117725 Knockout...

  13. Promega Plasmid Collection

    Type
    Collection
    ...HaloTag, SmBiT, and LgBiT, useful for detecting and tracking key drug target proteins....analysis and protein fusion vectors for detecting and tracking proteins using tags such as NanoLuc, HaloTag, ...signaling pathways, investigating RNA regulation, tracking protein degradation, analyzing CRISPR-mediated...presence of a substrate. NanoBiT PPI assays enable kinetic, real-time analysis of protein interaction, association...

  14. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. ...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

  15. Antibody Guide

    Type
    Collection
    ...important factor (for instance, looking for a different conjugate or looking for antibodies validated for...unused surface-binding sites in the wells with a blocking protein such as BSA, followed by several wash ... assay, called native ChIP, does not use cross-linking and instead relies on strong interactions between...the product information for similar antibodies, looking for ones that have identical features and epitopes...

  16. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... does not require a specific flanking sequence on the target RNA, making it a very flexible system. Other...rather than DNA, sometimes requiring a protospacer flanking sequence (PFS). In bacteria, Cas13 targeting also...):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide sequence...

  17. Plasmids for Stem Cell Research

    Type
    Collection
    ... vector for "hit and run" reprogramming of adult skin fibroblasts to induced pluripotent stem cells. Stem...non-integrating episomes to allow sorting and dosage tracking of reprogramming factors Fluorescent tagged episomals...Stem Cell. 2012 Apr 6;10(4):465-72. Schöler Adult Skin Fibroblasts Cholinergic Neurons Lentiviral Human...Cell Stem Cell. 2015 Nov 5;17(5):543-56. Buganim Skin Fibroblasts Motor Neurons Lentiviral Human Direct...

  18. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...pAAV-AiE2150m-minBG-SYFP2-WPRE3-BGHpA AiP1379 AiE2150m SYFP2 Purkinje cells Cerebellum 208141 pAAV-AiE0390m_3xC2-minBG-SYFP2...Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith KA, Esposito L, Waters J, Thyagarajan...Smith K, Way SW, Esposito L, Yao Z, Gradinaru V, Sunkin SM, Lein E, Levi BP, Ting JT, Zeng H, Tasic B. ... C, Ko AL, Ojemann JG, Keene CD, Silbergeld DL, Sunkin SM, Gradinaru V, Horwitz GD, Zeng H, Tasic B, Lein...

  19. Plan Your Experiment

    Type
    Collection
    ...setting up CRISPR experiments. We will focus on making single edits using CRISPR/Cas9 in mammalian cells...experiments and can be used as positive controls when working out a new protocol. Read more about how to design... the cell type. Before proceeding, we recommend asking labmates/colleagues, searching the literature, ...efficiency. Edit Type The type of edit you are looking for will ultimately depend on which CRISPR method...

  20. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...finger nucleases (ZFNs) are effective tools for making targeted mutations in zebrafish. With support ...OZ526 OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527 and OZ528 OPEN OPEN...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al....
Showing: 41 - 60 of 932 results