We narrowed to 731 results for: Sis

...reporter vectors for sensitive gene expression analysis and fusion vectors containing tags such as NanoLuc...reporter vectors for sensitive gene expression analysis and protein fusion vectors for detecting and tracking.... NanoBiT PPI assays enable kinetic, real-time analysis of protein interaction, association, and dissociation...

...72258 Expresses FLPe, copGFP and the puromycin resistance from the CB promoter pCDH-CB-FLPe-P2A-tdTomato...copGFP-T2A-Puro 72263 Express copGFP and the puromycin resistance gene from the EF1 promoter pCDH-CMV-mCherry-T2A-Puro...-T2A-Puro 72264 Express mCherry and puromycin resistance gene from the CMV promoter pCDH-CMV 72265 Express...

...opens in a new window) The Open Enzyme Collection consists of more than 75 genes for enzymes that are considered...reverse transcription of RNA into DNA, and dNTP synthesis. Additionally, the Open Enzyme collection contains...coli . Researchers can use the collection as the basis of manufacturing their own enzymes by cloning them...

...preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver staining or SYPRO...purified and non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are...preparation shows that the vast majority of the vectors consist of full particles (white arrowhead) relative to...

... Minibodies are engineered antibody fragments consisting of two scFvs bound to a CH3 domain. Fab fragments...specific proteins or protein complexes for downstream analysis. They are powerful tools for understanding protein...complex and can be sequenced and amplified for analysis. Overview of application Process tissue or cell...crosslink, isolate DNA, and perform downstream analysis. Special considerations One type of ChIP assay...

...2015. 17(2): p. 178-94. PubMed PMID: 26189426. 15. Si-Tayeb, K., et al., Highly efficient generation of...successfully [10-12]. The next stage of reprogramming consists of redifferentiation of iPSCs into the cell type...

...Anhydrotetracycline (aTc) Escherichia coli , Mycobacterium tuberculosis Sabine Ehrt 44561 pST-KT Pmyc1/TetO Anhydrotetracycline...Anhydrotetracycline (aTc) Escherichia coli , Mycobacterium tuberculosis Vinay Nandicoori 78577 pL99 PnitA-NitR ε-caprolactam...Pnark2 Hypoxia Escherichia coli , Mycobacterium tuberculosis Vinay Nandicoori 188974 pREDusk-AmpR-MCS FixK2...

...promotes human nuclear reprogramming and regulates mitosis. Nat Commun. 2016 Mar 7;7:10869. Hu CRISPRa Human...induced Beta Cells Adenoviral Mouse Long-term persistence and development of induced pancreatic beta cells...iPSCs Bipolar Neurons Lentiviral Human Rapid neurogenesis through transcriptional activation in human ...

...factors for cellular programming or cancer drug resistance....applications such as cellular programming or cancer drug resistance. Protocols & References A transcription factor...

...vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled library from the Hahn and Root...Library (Frank Stegmeier) Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris...

...1000000013 ) Depositing Labs: Keith Joung This kit consists of plasmids and strains used to engineer ZFNs ...Oligomerized Pool ENgineering (OPEN) method. This kit consists of 5 individually packaged plasmids and 3 individually...

...If any discrepancies are identified during this analysis, you will be notified by email. If Addgene determines... the material received. Shipping While Addgene assists importation by including thorough shipping documents...

...cells. See the Weinberg Lab page for additional resistance variants. Bob Weinberg 1773 pBABE-hygro-hTERT...GFP expression. See plasmid 8452 for puromycin resistance. William Hahn 63704 pRetroX GFP T2A Cre CMV/MSV...

...yeast, plants, or insect cells. Benefits include consistency between lots and the ability to optimize the ... vector facilitating production and functional analysis of scFv antibody fragments selected from Tomlinson...

... sp. Bacillus sp. Burkholderia sp. Bordetella pertussis Campylobacter sp. Chlamydia sp. Clostridium sp...Saccharomyces Cerevisiae Archive for Functional Analysis (EUROSCARF) (Link opens in a new window) JGI Integrated...

...Brown W, Bardhan A, Delaney M, Ilk AS, Rauen RR, Kahn SI, Tsang M, Deiters A (2020) Spatiotemporal Control...protein combination. The optimized LACE system consisted of: 1) CIBN-dCas9-CIBN, where CIBN is the N-terminal...(Kawano et al., 2015). Nicknamed paCas9-1 and consisting of the fusion proteins N713-pMag and nMagHigh1...Michael Lin, who developed a chimeric protein consisting of Cas9 and a photo-dissociable dimeric green...

...lymphoma invasion and metastasis TP53 Tumor protein p53 TSC TSC1 TSC2 Tuberous sclerosis VAV1 Vav guanine ...

...EGFP 3rd CMV-driven EGFP fusion with puromycin resistance. Can be used for cDNA expression. David Sabatini...FastFUCCI-Puro 3rd Encodes FUCCI reporters for cell cycle analysis. Puromycin selection. Kevin Brindle and Duncan...

...endogenous proteins. This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and...) microhomologies as homology arms. The system consists of two components: An all-in-one CRISPR/Cas9 vector...

... signal, sensitivity, wide dynamic range, and resistance to photobleaching have made luciferase a popular...proteome stress. pHLuc : An extracellular pH sensor consisting of a pH-sensitive GFP (superecliptic pHluorin...