... with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. ...

...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive overexpression...

... community demonstrating AAV performance under various experimental conditions. Protocols Written and ...and scientists across the research community on various aspects of viral vectors, including troubleshooting...

...Addgene's CRISPR collections feature plasmids for a variety of organisms, including mammals, plants, bacteria...an empty vector for your experiment based on a variety of factors. gRNAs gRNA plasmids to target specific...

...immortalized cells (see Weinberg Lab page for resistance variants) Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression...expression; also expresses GFP. Also see pMIG-w , a variant that has WRE, a post-transcriptional regulatory...

...applications. The specific QC experiments performed varies for each antibody catalog item. Whenever possible...primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified, sequenced...

...mTORC2 The better-characterized mTORC1 responds to various signals, including amino acids, stress, oxygen,...prognosis in many cancers, including breast, colon, ovarian, and liver cancers. mTORC1 is activated downstream...

...standards and typical titers, though actual yields may vary by construct. Size Quantity Total Volume Minimum...processing your order. MTA Approval 1+ business days, varies by requesting organization Viral vector preps remain...

...: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget...Germline TP53 mutations and Li-Fraumeni syndrome. Varley JM. Hum Mutat. 2003 Mar;21(3):313-20. PubMed PMID...

...express your gene of interest under the control of a variety of promoters. Professor Oka and the Baylor College...can be used to generate adenovirus expressing a variety of reporters as well as the Cre and Flp recombinases...

...AAV6 Vectors: Strategies for Overcoming Donor-Variation and Implications in Genome Editing. Sci Rep . ...

...expression once it is induced. Addgene has a wide variety of Cre recombinase plasmids. Special features include...6xHis Cre fused to supernegatively charged GFP variant T7 Bacterial Liu 62657 pAYC iCre CAG Mammalian ...9xGGS-Cre-6xHis Cre fused to highly cationic GFP variant T7 Bacterial Liu 62939 pET-Cre-6xHis Cre-6xHis ...

...combination of gene variation and lifestyle or environmental factors. For example, variations of Apolipoprotein...

...create cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection of Stem ...c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming to pluripotency...

...Enhancers were evaluated in mouse brains, using various delivery methods including intracerebroventricular...contains best-in-class AAV plasmids to target a variety of cell populations in different brain regions....

...science. General Guidelines Acrylamide percentage varies in SDS-PAGE gels. Use lower percentage acrylamide...buffer. Pro-Tips The volume of lysis buffer will vary depending on the size of your cell pellet but will...between 250–1000 µL . The ideal lysis buffer will vary depending on the cellular location of the protein...sample. Pro-Tip The ideal total protein loaded will vary between samples and target proteins but is typically... 1X TBST. Pro-Tip The ideal blocking buffer will vary between antibodies. Refer to the manufacturer's ...blocking buffer. Pro-Tip The optimal concentration will vary between antibodies but is typically between 1–10...

...increases reproducibility, as it eliminates the variation associated with repeated transient transfection...lots can promote or inhibit transfection. Test a variety of brands and FBS lot numbers to find one suitable...transduction efficiency. Infection efficiency will vary between cell lines. It is not recommended that lentiviral...that already contain 0.5 mL of virus solutions at various dilution. Make sure to use the polybrene-containing...transgene has integrated in different locations in the various cells in the culture. This is because lentiviral...of the lentivirus used, cells may also exhibit varying numbers of integration events. In other words, ... transgene in their genome. This can result in varying expression levels of the transgene from different...

...will vary depending on the sample type and the protein of interest. You may need to try a variety of fixation... Pro-Tip The optimal antibody concentration will vary but generally ranges from 1-10 µg/mL. Add 500 µL... target. The optimal antibody concentration will vary between antibodies. Review the manufacturer's instructions...

...reagents. Once produced, lentivirus can be used for a variety of downstream applications such as stable-cell ...FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one suitable ... cm dish. Pro-Tip There can be batch to batch variation when making the PEI working stock, therefore the...transfect cells with a fluorescent plasmid using a variety of ratios. Check the cells 1-2 days after transfection...

...Molecules absorb different wavelengths of light to varying degrees and most have a specific wavelength that...spectrophotometer between samples if they are expected to vary significantly in concentration. DNA dissolved in...in water is going to have a greater variance in concentration readings than a DNA sample dissolved in ...